LOCUS       sequence-392126-        4356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-OC43 NSP14_nostop
FEATURES             Location/Qualifiers
     source          1..4356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1667..1766)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1783..1802)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1784..1802)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1807..1823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1807..1823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2254..3045
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3138..3726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3627..3646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3880..3897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4056..4083)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4175..4261)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4310..4332
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4324..4341
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4325..4341
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtgtagtac taatttattt
      121 aaagattgta gcaagagtta tagcggttat cacccagctc atgctccttc atttttggca
      181 gtagatgaca aatataaggc aactggcgat ttagccgtgt gtcttggtat tggcgattct
      241 gctgttacat attcaagatt aatatcactc atgggtttta aattggatgt tacccttgat
      301 gggtattgta agctttttat aactaaagaa gaagctgtta aacgcgtgcg tgcctgggtt
      361 ggctttgatg ctgaaggtgc tcatgccacg cgtgatagca ttgggacaaa tttcccactt
      421 caattaggat tttccacagg aattgatttt gttgtggaag ccactggttt gtttgctgat
      481 agagatggtt acagctttaa aaaggctgtg gcgaaagctc ctcctggtga acaatttaag
      541 cacctcatcc ctttgatgac gagaggtcat cgctgggatg ttgttagacc tagaatagta
      601 caaatgtttg cagatcattt aattgatctg tctgattgtg ttgtgctagt tacatgggca
      661 gccaactttg agctcacttg tctccgctac tttgcaaaag tagggcgtga gatttcttgt
      721 aatgtatgca ctaaacgtgc cacagtttac aattctagaa ctggttacta tggttgttgg
      781 cgccatagtg ttacatgtga ttacttgtat aatccactta ttgttgatat tcaacagtgg
      841 ggatatattg gttctttatc aagtaatcat gatttatatt gtagtgtcca taaaggagca
      901 catgttgctt cctctgatgc tataatgaca cggtgtttgg ccgtttatga ttgcttttgc
      961 aataatatta attggaatgt ggagtatccc atcatttcaa atgagttaag tattaatacc
     1021 tcttgtaggg tcttgcagcg tgtgattctt aaagctgcca tgctctgcaa cagatatact
     1081 ttgtgttatg atattggcaa cccaaaagcg attgcctgtg tcaaagattt tgattttaag
     1141 ttctatgatg cccaaccaat tgttaagtct gttaagactc ttttgtattc ttttgaggca
     1201 cataaggact cttttaaaga cggtttgtgt atgttttgga actgtaatgt ggataagtat
     1261 ccaccgaatg cagttgtatg tagatttgac actagagtgt tgaataattt aaatcttcct
     1321 ggctgtaatg gaggtagttt gtatgttaat aaacatgcat tccacactaa accctttgct
     1381 agggcagcct ttgagcattt gaagcctatg ccattcttct attattcaga tacgccttgt
     1441 gtgtatatgg atggcatgga tgctaagcag gttgattatg tacctttgaa atctgccacg
     1501 tgcatcacaa gatgcaattt aggtggtgca gtttgtttaa aacatgctga agagtatcgt
     1561 gagtacttag agtcttacaa tacagctact acagcaggtt ttactttttg ggtctataag
     1621 acatttgatt tttataattt gtggaatacg ttcaccaagc tacaataccc aactttcttg
     1681 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1741 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1801 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1861 attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc
     1921 tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     1981 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     2041 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     2101 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     2161 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     2221 cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta
     2281 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     2341 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     2401 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     2461 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     2521 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     2581 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     2641 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     2701 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     2761 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     2821 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     2881 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     2941 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     3001 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca
     3061 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3121 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3181 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3241 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3301 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3361 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3421 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3481 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3541 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3601 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3661 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3721 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3781 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3841 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3901 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3961 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     4021 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     4081 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     4141 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     4201 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     4261 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     4321 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
