LOCUS       sequence-392131-        1687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK188
FEATURES             Location/Qualifiers
     source          1..1687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..31
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(52..86)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(157..816)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     731..750
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      998..1586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1487..1506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcccatcatc accatcatca ctaatggctg agaccagacc aataaaaaac
       61 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
      121 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
      181 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      241 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      301 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      361 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
      421 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      481 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      541 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
      601 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
      661 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      721 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      781 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      841 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      901 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
      961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1681 accgtag
//
