LOCUS       sequence-392134-        1717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK111
FEATURES             Location/Qualifiers
     source          1..1717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..61
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     terminator      complement(82..116)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(187..846)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     761..780
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1028..1616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1517..1536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccagagatc atatggtttt gcatgaatat gttaatgctg ctggtattac
       61 ttaatggctg agaccagacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      121 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
      181 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
      241 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
      301 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
      361 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg aaacgaaaaa
      421 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
      481 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
      541 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
      601 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
      661 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
      721 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
      781 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
      841 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
      901 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
      961 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1681 ttcctgcgtt atcccctgat tctgtggata accgtag
//
