LOCUS       sequence-392146-        1681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK107
FEATURES             Location/Qualifiers
     source          1..1681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(46..80)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(151..810)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     725..744
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      992..1580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1481..1500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tcccatgatg aattgtaatg gctgagacca gaccaataaa aaacgcccgg
       61 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
      121 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
      181 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      241 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      301 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      361 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
      421 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      481 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      541 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
      601 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
      661 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
      721 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
      781 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
      841 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
      901 tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1681 g
//
