LOCUS       sequence-392147-        5580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-OC43 NSP12_stop
FEATURES             Location/Qualifiers
     source          1..5580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2891..2990)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3007..3026)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3008..3026)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3031..3047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3031..3047)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3478..4269
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4362..4950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4851..4870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5104..5121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5280..5307)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5399..5485)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5534..5556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5548..5565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5549..5565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtcaaaaga tactaatttt
      121 ttaaaccggg ttcggggtac gagtgtagat gcccgtctcg taccctgcgc cagtggttta
      181 tctactgatg tacaattaag ggcatttgat atttacaatg ctagtgttgc tggcattggt
      241 ttacatttaa aagttaattg ttgccgtttt cagcgtgttg atgagaacgg tgataaatta
      301 gatcagttct ttgttgttaa gaggacagat ctgactatat ataatagaga gatgaaatgc
      361 tatgagcgtg taaaagattg taagtttgtg gctgaacacg atttctttac atttgatgta
      421 gaaggtagtc gtgtgccaca cattgtacgc aaggatttaa caaagtatac tatgttggat
      481 ctttgctatg cattgcgaca ttttgatcgc aatgattgca tgctgctttg tgacattctc
      541 tctatatatg ctggttgtga acaatcctac tttactaaga aggattggta tgattttgtt
      601 gaaaatcctg atattattaa tgtgtataaa aagctaggac ctatttttaa tagagcccta
      661 gttagcgcta ctgagtttgc ggacaaattg gtggaggtag gcttagtagg cgttttaaca
      721 cttgataatc aagatttaaa tggtaaatgg tatgattttg gtgactatgt tattgcagcc
      781 ccaggatgtg gtgttgctat agcagattct tattattctt atatcatgcc tatgctgacc
      841 atgtgtcatg cattggattg cgaattgtat gtgaataatg cttatagact atttgatctt
      901 gtacagtatg attttactga ttacaagctt gaattgttta ataagtattt taagcactgg
      961 agtatgccat atcatcctaa cactgttgat tgtcaggatg atcggtgtat tatacattgt
     1021 gctaatttta acatactttt tagtatggtt ttacctaata catgttttgg gcctcttgtt
     1081 aggcaaattt ttgtggatgg tgtgcctttt gttgtttcaa ttggctacca ttataaagaa
     1141 cttggtattg tgatgaatat ggatgtggat acacatcgtt atcgcttgtc tttaaaagac
     1201 ttgcttttat atgctgctga tccagctttg catgtagctt ctgctagtgc attgtatgat
     1261 ttacgcactt gctgttttag tgttgccgct ataacaagcg gtgtaaaatt tcaaacagtt
     1321 aaacctggta attttaatca ggatttttat gattttgttt taagtaaagg cctgcttaaa
     1381 gagggtagct cagttgatct gaagcacttt ttctttacac aggatggtaa tgctgctatt
     1441 actgattata attattataa gtataatttg cccaccatgg tggacattaa gcagttgttg
     1501 tttgttttgg aagttgttta taagtatttt gagatttatg atggtgggtg tataccggca
     1561 tcacaagtca ttgttaataa ttatgataag agtgctggct atccatttaa caaatttgga
     1621 aaagccaggc tctattatga agcattatca tttgaggaac aggatgaaat ttacgcttat
     1681 actaagcgta atgtcctgcc aacacttact caaatgaatt tgaaatatgc tattagtgct
     1741 aagaatagag cccgcactgt tgctggtgtt tccatactta gtactatgac tggcagaatg
     1801 tttcatcaaa aatgtttgaa aagtatagca gctacacgtg gtgttcctgt agttataggc
     1861 accactaaat tttatggtgg ctgggatgat atgttacgcc gccttattaa agatgttgac
     1921 aatcctgtac ttatgggttg ggattatcct aagtgtgatc gtgctatgcc aaacctacta
     1981 cgtattgtta gtagtttggt attagcccga aaacatgaga catgttgttc gcaaagcgat
     2041 aggttttatc gacttgcgaa tgaatgcgca caagttttga gtgaaattgt tatgtgtggt
     2101 ggctgttatt atgttaagcc tggtggcact agtagtggtg atgcaactac tgcttttgct
     2161 aattcagtct ttaacatatg tcaagctgtt tcagccaatg tatgtgcctt aatgtcatgc
     2221 aatggcaata agattgaaga tcttagtata cgtgctcttc agaagcgctt atactcacat
     2281 gtgtatagaa gtgataaggt tgattcaacc tttgtcacag aatattatga atttttaaat
     2341 aagcatttta gtatgatgat tttgagtgat gatggggttg tgtgttataa ttctgattat
     2401 gcgtccaaag ggtatattgc taatataagt gcctttcaac aggtattata ttatcaaaat
     2461 aacgttttta tgtcagaatc caaatgttgg gttgaacatg acataaataa tggacctcat
     2521 gaattctgtt cacaacacac aatgcttgta aagatggatg gtgacgatgt ctaccttcca
     2581 tatcctaatc ctagtcgtat attaggagct ggatgttttg tagatgattt gttaaagact
     2641 gatagtgttc ttttaataga acgatttgta agtcttgcaa tagatgctta tccacttgtg
     2701 tatcatgaaa atgaagaata ccaaaaggtt tttcgtgttt atttggcgta tataaagaag
     2761 ttgtacaatg acctgggtaa tcagatcttg gatagctaca gtgttatttt aagtacttgt
     2821 gatggacaaa agttcactga tgagtccttt tacaagaaca tgtatttaag aagtgcagtt
     2881 atgcagtaat acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     2941 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     3001 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     3061 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     3121 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     3181 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     3241 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     3301 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     3361 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     3421 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     3481 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     3541 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     3601 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     3661 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     3721 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     3781 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     3841 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     3901 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     3961 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     4021 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     4081 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     4141 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     4201 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     4261 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     4321 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4381 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4441 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     4501 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     4561 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     4621 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4681 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4741 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4921 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4981 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5041 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     5161 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     5221 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     5281 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     5341 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     5401 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     5461 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     5521 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
