LOCUS       sequence-392148-        4359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-OC43 NSP14_stop
FEATURES             Location/Qualifiers
     source          1..4359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1670..1769)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1786..1805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1787..1805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1810..1826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1810..1826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2257..3048
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3141..3729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3630..3649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3883..3900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4059..4086)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4178..4264)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4313..4335
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4327..4344
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4328..4344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtgtagtac taatttattt
      121 aaagattgta gcaagagtta tagcggttat cacccagctc atgctccttc atttttggca
      181 gtagatgaca aatataaggc aactggcgat ttagccgtgt gtcttggtat tggtgattct
      241 gctgttacat attcaagatt aatatcactc atgggtttta aattggatgt tacccttgat
      301 gggtattgta agctttttat aactaaagaa gaagctgtta aacgcgtgcg tgcctgggtt
      361 ggctttgatg ctgaaggtgc tcatgccacg cgtgatagca ttgggacaaa tttcccactt
      421 caattaggat tttccacagg aattgatttt gttgtggaag ccactggttt gtttgctgat
      481 agagatggtt acagctttaa aaaggctgtg gcgaaagctc ctcctggtga acaatttaag
      541 cacctcatcc ctttgatgac gagaggtcat cgctgggatg ttgttagacc tagaatagta
      601 caaatgtttg cagatcattt aattgatctg tctgattgtg ttgtgctagt tacatgggca
      661 gccaactttg agctcacttg tctccgctac tttgcaaaag tagggcgtga gatttcttgt
      721 aatgtatgca ctaaacgtgc cacagtttac aattctagaa ctggttacta tggttgttgg
      781 cgacatagtg ttacatgtga ttacttgtat aatccactta ttgttgatat tcaacagtgg
      841 ggatatattg gttctttatc aagtaatcat gatttatatt gtagtgtcca taaaggagca
      901 catgttgctt cctctgatgc tataatgaca cggtgtttgg ccgtttatga ttgcttttgc
      961 aataatatta attggaatgt ggagtatccc atcatttcaa atgagttaag tattaatacc
     1021 tcttgtaggg tcttgcagcg tgtgattctt aaagctgcca tgctctgcaa cagatatact
     1081 ttgtgttatg atattggcaa cccaaaagcg attgcctgtg tcaaagattt tgattttaag
     1141 ttctatgatg cccaaccaat tgttaagtct gttaagactc ttttgtattc ttttgaggca
     1201 cataaggact cttttaaaga cggtttgtgt atgttttgga actgtaatgt ggataagtat
     1261 ccaccgaatg cagttgtatg tagatttgac actagagtgt tgaataattt aaatcttcct
     1321 ggctgtaatg gaggtagttt gtatgttaat aaacatgcat tccacactaa accctttgct
     1381 agggcagcct ttgagcattt gaagcctatg ccattcttct attattcaga tacgccttgt
     1441 gtgtatatgg atggcatgga tgctaagcag gttgattatg tacctttgaa atctgccacg
     1501 tgcatcacaa gatgcaattt aggtggtgca gtttgtttaa aacatgctga agagtatcgt
     1561 gagtacttag agtcttacaa tacagctact acagcaggtt ttactttttg ggtctataag
     1621 acatttgatt tttataattt gtggaatacg ttcaccaagc tacaataata cccaactttc
     1681 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1741 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1801 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1861 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     1921 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     1981 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     2041 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     2101 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     2161 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     2221 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     2281 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     2341 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     2401 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     2461 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     2521 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     2581 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     2641 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     2701 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     2761 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2821 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     2881 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2941 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     3001 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     3061 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4021 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4081 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4141 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4201 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4261 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4321 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
