LOCUS       sequence-392149-        1726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 4a part for Modular Cloning in Yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Simakin P, Koch C, Herrmann JM
  TITLE     A modular cloning (MoClo) toolkit for reliable intracellular protein
            targeting in the yeast Saccharomyces cerevisiae.
  JOURNAL   Microb Cell. 2023 Feb 28;10(4):78-87. doi: 10.15698/mic2023.04.794. 
            eCollection 2023 Apr 3.
  PUBMED    37009624
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15698/mic2023.04.794"; journalName: "Microb Cell"; date: 
            "2023-02-28- 28"; volume: "10"; issue: "4"; pages: "78-87"
COMMENT     Sequence Label: pHHYTK109
FEATURES             Location/Qualifiers
     source          1..1726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             47..67
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(91..125)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(196..855)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     770..789
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1037..1625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1526..1545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccaaaagaa ctgctgatgg ttctgaattt gaatctccaa aaaaaaaaag
       61 aaaagttgaa taatggctga gaccagacca ataaaaaacg cccggcggca accgagcgtt
      121 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
      181 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
      241 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
      301 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
      361 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
      421 aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
      481 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
      541 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
      601 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
      661 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
      721 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
      781 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
      841 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
      901 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa
      961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
