LOCUS       sequence-392173-        6312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-229E S_stop
FEATURES             Location/Qualifiers
     source          1..6312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3623..3722)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3739..3758)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3740..3758)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3763..3779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3763..3779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4210..5001
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5094..5682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5583..5602
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5836..5853
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6012..6039)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6131..6217)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     6266..6288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6280..6297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6281..6297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgtttgtttt gcttgttgca
      121 tatgccttgt tgcatattgc tggttgtcaa actacaaatg ggctgaacac tagttactct
      181 gtttgcaacg gctgtgttgg ttattcagaa aatgtatttg ctgttgagag tggtggttat
      241 ataccctccg actttgcatt caataattgg ttccttctaa ctaatacctc atctgttgta
      301 gatggtgttg tgaggagttt tcagcctttg ttgcttaatt gcttatggtc tgtttctggc
      361 ttgcggttta ctactggttt tgtctatttt aatggtactg ggagaggtga ttgtaaaggt
      421 ttttcctcag atgttttgtc tgatgtcata cgttacaacc tcaattttga agaaaacctt
      481 agacgtggaa ccattttgtt taaaacatct tatggtgttg ttgtgtttta ttgtaccaac
      541 aacactttag tttcaggtga tgctcacata ccatttggta cagttttggg caatttttat
      601 tgctttgtaa atactactat tggcaatgaa actacgtctg cttttgtggg tgcactacct
      661 aagacagttc gtgagtttgt tatttcacgc acaggacatt tttatattaa tggctatcgc
      721 tatttcactt taggtaatgt agaagccgtt aatttcaatg tcactactgc agaaaccact
      781 gatttttgta ctgttgcgtt agcttcttat gctgacgttt tggttaatgt gtcacaaacc
      841 tctattgcta atataattta ttgcaactct gttattaaca gactgagatg tgaccagttg
      901 tcctttgatg taccagatgg tttttattct acaagcccta ttcaatccgt tgagctacct
      961 gtgtctattg tgtcgctacc tgtttatcat aaacatacgt ttattgtgtt gtacgttgac
     1021 ttcaaacctc agagtggcgg tggcaagtgc tttaactgtt atcctgctgg tgttaatatt
     1081 acactggcca attttaatga aactaaaggg cctttgtgtg ttgacacatc acacttcact
     1141 accaaatacg ttgctgttta tgccaatgtt ggtaggtgga gtgctagtat taacacggga
     1201 aattgccctt tttcttttgg caaagttaat aactttgtta aatttggcag tgtatgtttt
     1261 tcgctaaagg atatacccgg tggttgcgca atgcctatag tggctaattg ggcttatagt
     1321 aagtactata ctataggctc attgtatgtt tcttggagtg atggtgatgg aattactggc
     1381 gtcccacaac ctgttgaggg tgttagttcc tttatgaatg ttacattgga caaatgtact
     1441 aaatataata tttatgatgt atctggtgtg ggtgttattc gcgttagcaa tgacaccttt
     1501 cttaatggaa ttacgtacac atcaacttca ggtaaccttc tgggttttaa agatgttact
     1561 aagggcacca tctactctat cactccttgt aacccaccag atcagcttgt tgtttatcag
     1621 caagctgttg ttggtgctat gttgtctgaa aattttacta gttacggctt ttctaatgtt
     1681 gtagaactgc cgaaattttt ctatgcgtcc aatggcactt ataattgcac agacgctgtt
     1741 ttaacttatt ctagttttgg cgtttgtgca gatggttcta taattgctgt tcaaccacgt
     1801 aatgtttcat atgatagtgt ttcagctatc gtcacagcta atttgtctat accttccaat
     1861 tggaccactt cggtccaggt tgagtattta caaattacaa gtacacctat cgtagttgat
     1921 tgctccactt atgtttgcaa tggtaatgtg cgctgtgttg aattgcttaa gcagtatact
     1981 tctgcttgta aaactattga agacgcctta agaaatagcg ccaggctgga gtctgcagat
     2041 gttagtgaga tgctcacttt tgacaagaaa gcgtttacac ttgctaatgt tagtagtttt
     2101 ggtgactaca accttagcag cgtcatacct agcttgccca caagtggtag tagagtggct
     2161 ggtcgcagtg ccatagaaga catacttttt agcaaacttg ttacttctgg acttggcact
     2221 gtggacgcag actacaaaaa gtgcactaag ggtctttcca ttgctgactt ggcttgtgct
     2281 caatattata atggcattat ggttttgcct ggcgtcgctg atgctgaacg aatggccatg
     2341 tatacaggtt ctttaattgg tggaattgct ttaggaggtc taacatcagc cgtttcaata
     2401 ccattttcat tagcaattca ggcacgttta aattatgttg cattgcagac tgatgtttta
     2461 caagaaaatc agaaaattct tgctgcatct tttaacaaag caatgaccaa catagtagat
     2521 gcctttactg gtgttaatga tgctattaca caaacttcac aagccctaca aacagttgct
     2581 actgcactta acaagatcca ggatgttgtt aatcaacaag gcaactcatt gaaccattta
     2641 acttctcagt tgaggcagaa ttttcaagct atctctagct ctattcaggc tatctatgac
     2701 agacttgaca ctattcaggc tgatcaacaa gtagataggc tgattactgg tagattggct
     2761 gctttgaatg tattcgtttc tcatacattg actaagtaca ctgaagttcg tgcttccaga
     2821 cagcttgcac aacaaaaagt gaatgagtgt gtcaaatccc agtctaagcg ttatggcttc
     2881 tgtggaaatg gcactcacat tttctcaatt gttaatgctg ctcctgaggg gcttgttttt
     2941 ctccacactg tcttgttgcc gacacaatat aaggatgttg aagcgtggtc tgggttgtgc
     3001 gttgatggta caaacggtta tgtgttgcga caacctaatc ttgctcttta caaagaaggc
     3061 aattattata gaatcacatc tcgcataatg tttgaaccac gtattcctac catggcagat
     3121 tttgttcaaa ttgaaaattg caatgtcaca tttgttaaca tttctcgctc tgagttgcaa
     3181 accattgtgc cagagtatat tgatgttaat aagacgctgc aagaattaag ttacaaattg
     3241 ccaaattaca ctgttccaga cctagttgtc gaacagtaca accagactat tttgaatttg
     3301 accagtgaaa ttagcaccct tgaaaataaa tctgcggagc ttaattacac tgttcaaaaa
     3361 ttgcaaactc tgattgacaa cataaatagc acattagtcg acttaaagtg gctcaaccgg
     3421 gttgagactt acatcaagtg gccgtggtgg gtgtggttgt gcatttcagt cgtgctcatc
     3481 tttgtggtga gtatgttgct attatgttgt tgttctactg gttgctgtgg cttctttagt
     3541 tgttttgcat cttctattag aggttgttgt gaatcaacta aacttcctta ttacgacgtt
     3601 gaaaagatcc acatacagta atacccaact ttcttgtaca aagttggcat tataagaaag
     3661 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     3721 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     3781 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     3841 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt
     3901 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     3961 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     4021 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     4081 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     4141 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     4201 ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     4261 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     4321 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     4381 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     4441 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     4501 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     4561 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     4621 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     4681 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     4741 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     4801 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4861 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4921 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     4981 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt
     5041 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5761 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5821 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5881 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5941 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     6001 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     6061 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     6121 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     6181 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     6241 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     6301 aagctcgggc cc
//
