LOCUS       sequence-39218-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene was not detected in the brain and 
            eye using LacZ. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1651
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..178
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      189..198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          248..380
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 cgcttctggt gccggaaacc aggcaaagcg ccattcgcca ttcaggctgc gcaactgttg
       61 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      121 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      181 gggatccgcc atggtggcgg aagttcctat accttttgaa gaataggaac ttcggaatag
      241 gaacttcctg tggagagaaa ggcaaagtgg atgtcagtaa gaccaatagg tgcctatcag
      301 aaacgcaaga gtcttctctg tctcgacaag cccagtttct attggtctcc ttaaacctgt
      361 cttgtaacct tgatacttac ggcgcgccga gctttccctc agaagacctt gttgactgtc
      421 ccaaagcaaa cctggtcctt gccaccacca gactccagcc cccagacctc ctgctgcctg
      481 cttcctctcc cctacaggaa attaataatc ttggtgtcca atcaagtgcc ctgttgctcc
      541 tgctttcccc cagcaaagct gggggggccg gggggtgtaa acttcttcct ctgcccccta
      601 aaatacaaca ccctactttg gctccctctt tcctttcctt taaagagatg tgaacccctg
      661 aagggctccg cactcctctt cttgtccttt ctctttccta acaaatcacc cctaattcca
      721 cctagcacgg gtgtttctga tcttggtact gtcctcttct cttctcttcc aagatgtaac
      781 aaaaacaacc cccacgcaca tctcagcccc t
//
