LOCUS       sequence-392205-        8883 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Elongation reporter construct to quantify elongation duration of 
            6_AGA stalling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Hou W, Harjono V, Harvey AT, Subramaniam AR, Zid BM
  TITLE     Quantification of elongation stalls and impact on gene expression in
            yeast.
  JOURNAL   RNA. 2023 Dec;29(12):1928-1938. doi: 10.1261/rna.079663.123. Epub 
            2023 Oct 2.
  PUBMED    37783489
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079663.123"; journalName: "RNA"; date: "2023-12"; 
            volume: "29"; issue: "12"; pages: "1928-1938"
COMMENT     Sequence Label: pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
FEATURES             Location/Qualifiers
     source          1..8883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2034..2430
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(2443..3063,3064..3450)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MPRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2516..2535)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      3471..3658
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3526..3550)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(3716..3734,3735..3757,3758..3776,3777..3799,3800..3818,
                     3819..3841,3842..3860,3861..3883,3884..3902,3903..3925,
                     3926..3944,3945..3967,3968..3986)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(5445..5463)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      5475..5664
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5713..5731)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5752..5768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5752..5768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5765..5787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5776..5792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5800..5806,5807..5824,5825..5830))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5845..5866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5983..6000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6154..6742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6234..6253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6913..7704,7705..7773))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7536..7555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7774..7878)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8856..8874
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctcagttt atcattatca atactcgcca tttcaaagaa taccaacttc
     2041 ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa
     2101 aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc
     2161 gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt
     2221 cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt
     2281 gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc
     2341 ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata
     2401 tttcaagcta taccaagcat acaatcaact atctcatcta gaatgcctag attagataaa
     2461 agtaaagtga ttaacagcgc attagagctg cttaatgggg tcggaatcga aggtttaaca
     2521 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     2581 aataagcggg ctttgctcga cgccttaccc attgagatgt tagataggca ccatactcac
     2641 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     2701 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     2761 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     2821 ctagagaatg cattatatgc actcagcgct gtggggcact ttactttagg ttgcgtattg
     2881 gaagaacaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     2941 ccgccattat tacgacaagc tatcgaatta tttgatcgcc aaggtgcaga gccagccttc
     3001 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     3061 tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc
     3121 gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg cctgtccttt
     3181 ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt cagcctgggg
     3241 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     3301 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     3361 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     3421 gcccttggaa ttgacgagta cggtgggtag ctcgagccac ttctaaataa gcgaatttct
     3481 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     3541 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     3601 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
     3661 gatccgctag ggataacagg gtaatataga tcaattcctc gatcgagttt accactccct
     3721 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     3781 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3841 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3901 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     3961 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc ctatggcatg
     4021 catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat attctttcct
     4081 tatacattag gtcctttgta gcataaatta ctatacttct atagacacgc aaacacaaat
     4141 acacacacta aattaccgga tcaattatgt ctaagggtga agaattgttc actggtgttg
     4201 ttccaatctt ggttgaattg gacggtgacg ttaacggtca caagttctct gtttctggtg
     4261 aaggtgaagg tgacgctact tacggtaagt tgactttgaa gttcatctgt actactggta
     4321 agttgccagt tccatggcca actttggtta ctactttggg ttacggtttg atgtgtttcg
     4381 ctagataccc agaccacatg aagcaacacg acttcttcaa gtctgctatg ccagaaggtt
     4441 acgttcaaga aagaactatc ttcttcaagg acgacggtaa ctacaagact agagctgaag
     4501 ttaagttcga aggtgacact ttggttaaca gaatcgaatt gaagggtatc gacttcaagg
     4561 aagacggtaa catcttgggt cacaagttgg aatacaacta caactctcac aacgtttaca
     4621 tcatggctga caagcaaaag aacggtatca aggctaactt caagatcaga cacaacatcg
     4681 aagacggttc tgttcaattg gctgaccact accaacaaaa cactccaatc ggtgacggtc
     4741 cagttttgtt gccagacaac cactacttgt cttaccaatc taagttgtct aaggacccaa
     4801 acgaaaagag agaccacatg gttttgttgg aattcgttac tgctgctggt atcactcacg
     4861 gtatggacga attgtacaag agaagaagaa gaagaagaat ggtttttact ttagaagatt
     4921 ttgttggtga ttggcgtcaa actgctggtt ataatttaga tcaagtttta gaacaaggtg
     4981 gtgtttcttc tttatttcaa aatttaggtg tttctgttac tcctattcaa cgtattgttt
     5041 tatctggtga aaatggttta aaaattgata ttcatgttat tattccttat gaaggtttat
     5101 ctggtgatca aatgggtcaa attgaaaaaa tttttaaagt tgtttatcct gttgatgatc
     5161 atcattttaa agttatttta cattatggta ctttagttat tgatggtgtt actcctaata
     5221 tgattgatta ttttggtcgt ccttatgaag gtattgctgt ttttgatggt aaaaaaatta
     5281 ctgttactgg tactttatgg aatggtaata aaattattga tgaacgttta attaatcctg
     5341 atggttcttt attatttcgt gttactatta atggtgttac tggttggcgt ttatgtgaac
     5401 gtattttagc taattctcat ggtttttaaa agcttattag ttatgtcacg cttacattca
     5461 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
     5521 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
     5581 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
     5641 gagaaggttt tgggacgctc gaaggcttta atttgcggcc gtcgaggggg ggcccggtac
     5701 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     5761 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5821 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5881 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5941 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6001 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6061 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6121 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6181 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6241 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6301 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6361 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6421 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6481 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6541 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6601 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6661 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6721 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6781 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6841 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6901 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6961 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7021 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7081 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7141 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7201 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7261 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7321 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7381 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7441 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7501 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7561 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7621 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7681 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7741 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7801 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7861 acaaataggg gttccgcgca catttccccg aaaagtgcca cctggggggt gctaattatg
     7921 gcattgattg gggcggtaaa acattcaaag tcaatgaaaa ggatgctaaa ttgaatgata
     7981 ctgaagacat ttcattcgct tctcagatca aaaacttaaa tctgtctata tgagctgtcc
     8041 caaagccact tcttaccttt caagaaaaca tggaccttgc tttgccacaa aataattgcc
     8101 tgtaagatag ttttttattt gcacattgtt attactaacc tatgctttgc tacggacgac
     8161 tgcttggtaa aaatgcaaaa gtattaggat gtctcacaag aaactgctcg tggtgaaaat
     8221 tttcagccgc gaccatcctc ccacgccaac aagccctctt ttgcccatcc ttctggaatt
     8281 cgcggtgaaa atcaatttga tatataaaat tccctcataa tatctaggaa cgggatcaca
     8341 tatctcaatg agctaaagcg gccgcttctt cgaatgggac cagctaaagt gatcaaactt
     8401 catgcaggat gttggacact ttttctttca agaagctcca aatatataaa tttttgcaat
     8461 ctaggaattg aaatatcgta cttccgattc agaatatctc aactctacta catctatata
     8521 tgactgtact cgcgttcttg gtaaacatca attagtaggt gtttttgcga gcatatgttg
     8581 agctcagaaa atatgagcac aatcttcgaa accattgttt ttttgtgcaa aagtcaccat
     8641 cgggtttata gtatggactc ttgaacgaac cattctgatc tgtatttctc tcatatttac
     8701 tccatttatt ttttgctgct ctttgcttta cttcaacacg atgcttttcc taagtaattt
     8761 tttattttat tcacaatgct ccataactct tcaggcctta aacgaccatc catggcccct
     8821 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8881 gtc
//
