LOCUS       sequence-392206-       10849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for
            amplifying fluorescent signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Xu H, Wang J, Deng Y, Hou F, Fu Y, Chen S, Zou W, Pan D, Chen B
  TITLE     Two-Color CRISPR Imaging Reveals Dynamics of Herpes Simplex Virus 1 
            Replication Compartments and Virus-Host Interactions.
  JOURNAL   J Virol. 2022 Dec 21;96(24):e0092022. doi: 10.1128/jvi.00920-22. 
            Epub 2022 Dec 1.
  PUBMED    36453882
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2022-12-21"; volume: "96(24):e0092022. doi"; pages: " 
            10.1128/jvi.00920-22. Epub 2022 Dec 1"
COMMENT     Sequence Label: pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD
FEATURES             Location/Qualifiers
     source          1..10849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2631..2651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2655..2681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2655..2681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2682..2693
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5865..5885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5928..5984
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6000..6056
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6072..6128
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6144..6200
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6216..6272
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6288..6344
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6360..6416
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6432..6488
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6504..6560
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6576..6632
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6678..6725
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6726..6749
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6759..6815
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6816..7214
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7239..7827
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7292..7312)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7710..7721)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7902..8135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8254..8272)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8340..8444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8445..8513,8514..9305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8663..8682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9476..10064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9965..9984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10218..10235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10475..10496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10511..10516,10517..10534,10535..10541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10549..10565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10554..10576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10573..10589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10573..10589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10639..10658
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgcaatgggc ccaaaaaaga
     2641 aaagaaaagt tggctacccc tacgacgtgc ccgactacgc catcgaaggc cgccatatga
     2701 agcggaacta catcctgggc ctggccatcg gcatcaccag cgtgggctac ggcatcatcg
     2761 actacgagac acgggacgtg atcgatgccg gcgtgcggct gttcaaagag gccaacgtgg
     2821 aaaacaacga gggcaggcgg agcaagagag gcgccagaag gctgaagcgg cggaggcggc
     2881 atagaatcca gagagtgaag aagctgctgt tcgactacaa cctgctgacc gaccacagcg
     2941 agctgagcgg catcaacccc tacgaggcca gagtgaaggg cctgagccag aagctgagcg
     3001 aggaagagtt ctctgccgcc ctgctgcacc tggccaagag aagaggcgtg cacaacgtga
     3061 acgaggtgga agaggacacc ggcaacgagc tgtccaccaa agagcagatc agccggaaca
     3121 gcaaggccct ggaagagaaa tacgtggccg aactgcagct ggaacggctg aagaaagacg
     3181 gcgaagtgcg gggcagcatc aacagattca agaccagcga ctacgtgaaa gaagccaaac
     3241 agctgctgaa ggtgcagaag gcctaccacc agctggacca gagcttcatc gacacctaca
     3301 tcgacctgct ggaaacccgg cggacctact atgagggacc tggcgagggc agccccttcg
     3361 gctggaagga catcaaagaa tggtacgaga tgctgatggg ccactgcacc tacttccccg
     3421 aggaactgcg gagcgtgaag tacgcctaca acgccgacct gtacaacgcc ctgaacgacc
     3481 tgaacaatct cgtgatcacc agggacgaga acgagaagct ggaatattac gagaagttcc
     3541 agatcatcga gaacgtgttc aagcagaaga agaagcccac cctgaagcag atcgccaaag
     3601 aaatcctcgt gaacgaagag gatattaagg gctacagagt gaccagcacc ggcaagcccg
     3661 agttcaccaa cctgaaggtg taccacgaca tcaaggacat taccgcccgg aaagagatta
     3721 ttgagaacgc cgagctgctg gatcagattg ccaagatcct gaccatctac cagagcagcg
     3781 aggacatcca ggaagaactg accaatctga actccgagct gacccaggaa gagatcgagc
     3841 agatctctaa tctgaagggc tataccggca cccacaacct gagcctgaag gccatcaacc
     3901 tgatcctgga cgagctgtgg cacaccaacg acaaccagat cgctatcttc aaccggctga
     3961 agctggtgcc caagaaggtg gacctgtccc agcagaaaga gatccccacc accctggtgg
     4021 acgacttcat cctgagcccc gtcgtgaaga gaagcttcat ccagagcatc aaagtgatca
     4081 acgccatcat caagaagtac ggcctgccca acgacatcat tatcgagctg gcccgcgaga
     4141 agaactccaa ggacgcccag aaaatgatca acgagatgca gaagcggaac cggcagacca
     4201 acgagcggat cgaggaaatc atccggacca ccggcaaaga gaacgccaag tacctgatcg
     4261 agaagatcaa gctgcacgac atgcaggaag gcaagtgcct gtacagcctg gaagccatcc
     4321 ctctggaaga tctgctgaac aaccccttca actatgaggt ggaccacatc atccccagaa
     4381 gcgtgtcctt cgacaacagc ttcaacaaca aggtgctcgt gaagcaggaa gaagccagca
     4441 agaagggcaa ccggacccca ttccagtacc tgagcagcag cgacagcaag atcagctacg
     4501 aaaccttcaa gaagcacatc ctgaatctgg ccaagggcaa gggcagaatc agcaagacca
     4561 agaaagagta tctgctggaa gaacgggaca tcaacaggtt ctccgtgcag aaagacttca
     4621 tcaaccggaa cctggtggat accagatacg ccaccagagg cctgatgaac ctgctgcgga
     4681 gctacttcag agtgaacaac ctggacgtga aagtgaagtc catcaatggc ggcttcacca
     4741 gctttctgcg gcggaagtgg aagtttaaga aagagcggaa caaggggtac aagcaccacg
     4801 ccgaggacgc cctgatcatt gccaacgccg atttcatctt caaagagtgg aagaaactgg
     4861 acaaggccaa aaaagtgatg gaaaaccaga tgttcgagga aaagcaggcc gagagcatgc
     4921 ccgagatcga aaccgagcag gagtacaaag agatcttcat caccccccac cagatcaagc
     4981 acattaagga cttcaaggac tacaagtaca gccaccgggt ggacaagaag cctaatagag
     5041 agctgattaa cgacaccctg tactccaccc ggaaggacga caagggcaac accctgatcg
     5101 tgaacaatct gaacggcctg tacgacaagg acaatgacaa gctgaaaaag ctgatcaaca
     5161 agagccccga aaagctgctg atgtaccacc acgaccccca gacctaccag aaactgaagc
     5221 tgattatgga acagtacggc gacgagaaga atcccctgta caagtactac gaggaaaccg
     5281 ggaactacct gaccaagtac tccaaaaagg acaacggccc cgtgatcaag aagattaagt
     5341 attacggcaa caaactgaac gcccatctgg acatcaccga cgactacccc aacagcagaa
     5401 acaaggtcgt gaagctgtcc ctgaagccct acagattcga cgtgtacctg gacaatggcg
     5461 tgtacaagtt cgtgaccgtg aagaatctgg atgtgatcaa aaaagaaaac tactacgaag
     5521 tgaatagcaa gtgctatgag gaagctaaga agctgaagaa gatcagcaac caggccgagt
     5581 ttatcgcctc cttctacaac aacgatctga tcaagatcaa cggcgagctg tatagagtga
     5641 tcggcgtgaa caacgacctg ctgaaccgga tcgaagtgaa catgatcgac atcacctacc
     5701 gcgagtacct ggaaaacatg aacgacaaga ggccccccag gatcattaag acaatcgcct
     5761 ccaagaccca gagcattaag aagtacagca cagacattct gggcaacctg tatgaagtga
     5821 aatctaagaa gcaccctcag atcatcaaaa agggcgaggg agctcccaag aaaaagcgca
     5881 aggtactcga gggcgccatc atacacgcgg ccgcaacaag cggtccggaa gaacttttga
     5941 gcaagaatta tcatcttgag aacgaagtgg ctcgtcttaa gaaaggttct ggcagtggag
     6001 aagaactgct ttcaaagaat taccacctgg aaaatgaggt agctagactg aaaaagggga
     6061 gcggaagtgg ggaggagttg ctgagcaaaa attatcattt ggagaacgaa gtagcacgac
     6121 taaagaaagg gtccggatcg ggtgaggagt tactctcgaa aaattatcat ctcgaaaacg
     6181 aagtggctcg gctaaaaaag ggcagtggtt ctggagaaga gctattatct aaaaactacc
     6241 acctcgaaaa tgaggtggca cgcttaaaaa agggaagtgg cagtggtgaa gagctactat
     6301 ccaagaatta tcatcttgag aacgaggtag cgcgtttgaa gaagggttcc ggctcaggag
     6361 aggaactgct ctcgaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagggat
     6421 cgggcagtgg tgaggaacta ctttcaaaga attaccacct cgaaaacgaa gtagctcgat
     6481 taaagaaagg ttcagggtcg ggtgaagaat tactgagtaa aaattatcat ctggaaaatg
     6541 aggtagcgag actaaaaaag gggagtggtt ctggcgagga attgctatcg aaaaattatc
     6601 atcttgagaa cgaagttgct aggctcaaaa agggctcagg ctcaggcaaa ggtccgggaa
     6661 gcggaggatc ctctagaaag cgacctgccg ccacaaagaa ggctggacag gctaagaaga
     6721 agaaagatta caaagacgat gacgataagg gatccggcgc aacaaacttc tctctgctga
     6781 aacaagccgg agatgtcgaa gagaatcctg gaccgatggc caagcctttg tctcaagaag
     6841 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     6901 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     6961 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     7021 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     7081 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     7141 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     7201 tgtgggaggg ctaatgtaca agtaaatcga tagatcctaa tcaacctctg gattacaaaa
     7261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     7321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     7381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     7441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     7501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7621 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7741 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7801 ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc
     7861 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7921 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7981 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8041 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8101 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8161 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa
     8221 ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     8281 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8461 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8521 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8581 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8641 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     8701 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8761 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8821 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8881 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8941 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9001 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9061 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9121 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9181 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9241 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9301 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9361 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9601 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10261 aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat
    10321 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10381 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc
    10441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10561 aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt
    10621 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    10681 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    10741 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    10801 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
