LOCUS       sequence-392216-        3089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA 
            region in dependent of homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu 
            Z, Zou W, Chen B
  TITLE     Real-time imaging of RNA polymerase I activity in living human 
            cells.
  JOURNAL   J Cell Biol. 2023 Jan 2;222(1):e202202110. doi: 
            10.1083/jcb.202202110. Epub 2022 Oct 25.
  PUBMED    36282216
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-01-2"; volume: "222(1):e202202110. doi"; pages: 
            " 10.1083/jcb.202202110. Epub 2022 Oct 25"
COMMENT     Sequence Label: pCA11-pMa-sgTS3-17xMS2V5-sgTS3
FEATURES             Location/Qualifiers
     source          1..3089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1334..1922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1414..1433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2093..2884,2885..2953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2716..2735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2954..3058)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacgtt
      241 agtaccgggc ccgctctaga gcaccgatgc tctccgagga gggaaggtga caaccggtaa
      301 cctacaaacg ggtggaggat caccccaccc gacacttcac aatcaagggg tacaatacac
      361 aagggtggag gaacacccca ccctccagac acattacaca gaaatccaat caaacagaag
      421 caccatcagg gcttctgcta ccaaatttat ctcaaaaaac tacaacaagg aatcaccatc
      481 agggattccc tgtgcaatat acgtcaaacg agggccacga cgggaggacg atcacgcctc
      541 ccgaatatcg gcatgtctgg ctttcgaatt cagtgcgtgg agcatcagcc cacgcagcca
      601 atcagagtcg aatacaagtc gactttcgcg aagagcatca gccttcgcgc cattcttaca
      661 caaaccacac tctcccctac aggaacagca tcagcgttcc tgcccagtac ccaactcaag
      721 aaaatttatg tccccatgca gcatcagcgc atgggcccca agaatacatc cccaacaaaa
      781 tcacatccga gcaccaacag ggctcggagt gttgtttctt gtccaactgg acaaaccctc
      841 catggaccat caggccatgg actctcacca acaagacaaa aactactctt ctcgaagcag
      901 catcagcgct tcgaaacact cgagcataca ttgtgcctat ttcttgggtg gacgatcacg
      961 ccacccatgc tctcacgaat ttcaaaacac ggacaaggac gagcaccacc agggctcgtc
     1021 gttccacgtc caatacgatt acttaccttt cgggatcacg atcacggatc ccgcagctac
     1081 atcacttcca ctcaggacat tcaagcatgc acgatcacgg catgctccac aagtctcaac
     1141 cacagaaact accaaatggg ttcagcacca gcgaacccac tcctacctca aacctcttcc
     1201 gcaccgatgc tctccgagga gggcggccgc gtggataacc gtattacggg ctaattcact
     1261 cccaacgaac tcacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2221 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2701 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3061 catttccccg aaaagtgcca cctgacgtc
//
