LOCUS       sequence-392222-       13842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCas13-VPR in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13842)
  AUTHORS   Liang Y, Xu H, Cheng T, Fu Y, Huang H, Qian W, Wang J, Zhou Y, Qian 
            P, Yin Y, Xu P, Zou W, Chen B
  TITLE     Gene activation guided by nascent RNA-bound transcription factors.
  JOURNAL   Nat Commun. 2022 Nov 28;13(1):7329. doi: 10.1038/s41467-022-35041-7.
  PUBMED    36443367
REFERENCE   2  (bases 1 to 13842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-11-28"; volume: "13(1):7329. doi"; pages: " 
            10.1038/s41467-022-35041-7"
COMMENT     Sequence Label: pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag
FEATURES             Location/Qualifiers
     source          1..13842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1809..1926
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2035..2338
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2616..3632
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3556..3578
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3640..3659
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3757..3777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7081..7101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7108..7257
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7282..7302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7732..8088
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8107..8676
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             8683..8736
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8737..9621
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     misc_feature    9693..10281
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9746..9766)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10164..10175)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10368..10601
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10673..10794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10710..10729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10764..10783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10834..10969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10989..11008)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10990..11008)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11018..11035)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11018..11034)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11027..11049)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11176..11631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11263..11282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11473..11494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11657..11761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11762..11830,11831..12622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11980..11999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12793..13381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13282..13301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13535..13552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13669..13690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13705..13710,13711..13728,13729..13735)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13743..13759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13748..13770
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13767..13783
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13767..13783
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13802..13822
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13804..13822
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1801 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1861 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1921 aattttatcg atcacgagac tagcctcgag aagcttgata attctgcagg aatctagtta
     1981 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2041 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2101 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2161 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2221 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     2281 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     2341 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     2401 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggs
     2461 gcgcgccrgg sggggsgggg sggggsgrgg ggsggggsgg ggsgrggsgg rgrggkgcgg
     2521 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2581 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
     2641 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     2701 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
     2761 aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc
     2821 ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg
     2881 cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg
     2941 cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     3001 gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg
     3061 tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt gctgagcacg
     3121 gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg
     3181 gggggtggcg gcaggkgggg gtgccgggcg gggcggggcc gcctcgggcc ggggrgggct
     3241 cgggggrggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     3301 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     3361 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     3421 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     3481 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     3541 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     3601 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3661 tgtctcatca ttttggcaaa gaattgattt gataccgcgg gcccgggatc tcgagctcaa
     3721 gcttcgaatt ctgcagtcga cggtaccgcc accatgccca agaagaagcg caaggtggga
     3781 cgcgtcaaca tccccgctct ggtggaaaac cagaagaagt actttggcac ctacagcgtg
     3841 atggccatgc tgaacgctca gaccgtgctg gaccacatcc agaaggtggc cgatattgag
     3901 ggcgagcaga acgagaacaa cgagaatctg tggtttcacc ccgtgatgag ccacctgtac
     3961 aacgccaaga acggctacga caagcagccc gagaaaacca tgttcatcat cgagcggctg
     4021 cagagctact tcccattcct gaagatcatg gccgagaacc agagagagta cagcaacggc
     4081 aagtacaagc agaaccgcgt ggaagtgaac agcaacgaca tcttcgaggt gctgaagcgc
     4141 gccttcggcg tgctgaagat gtacagggac ctgaccaacg catacaagac ctacgaggaa
     4201 aagctgaacg acggctgcga gttcctgacc agcacagagc aacctctgag cggcatgatc
     4261 aacaactact acacagtggc cctgcggaac atgaacgaga gatacggcta caagacagag
     4321 gacctggcct tcatccagga caagcggttc aagttcgtga aggacgccta cggcaagaaa
     4381 aagtcccaag tgaataccgg attcttcctg agcctgcagg actacaacgg cgacacacag
     4441 aagaagctgc acctgagcgg agtgggaatc gccctgctga tctgcctgtt cctggacaag
     4501 cagtacatca acatctttct gagcaggctg cccatcttct ccagctacaa tgcccagagc
     4561 gaggaacggc ggatcatcat cagatccttc ggcatcaaca gcatcaagct gcccaaggac
     4621 cggatccaca gcgagaagtc caacaagagc gtggccatgg atatgctcaa cgaagtgaag
     4681 cggtgccccg acgagctgtt cacaacactg tctgccgaga agcagtcccg gttcagaatc
     4741 atcagcgacg accacaatga agtgctgatg aagcggagca gcgacagatt cgtgcctctg
     4801 ctgctgcagt atatcgatta cggcaagctg ttcgaccaca tcaggttcca cgtgaacatg
     4861 ggcaagctga gatacctgct gaaggccgac aagacctgca tcgacggcca gaccagagtc
     4921 agagtgatcg agcagcccct gaacggcttc ggcagactgg aagaggccga gacaatgcgg
     4981 aagcaagaga acggcacctt cggcaacagc ggcatccgga tcagagactt cgagaacatg
     5041 aagcgggacg acgccaatcc tgccaactat ccctacatcg tggacaccta cacacactac
     5101 atcctggaaa acaacaaggt cgagatgttt atcaacgaca aagaggacag cgccccactg
     5161 ctgcccgtga tcgaggatga tagatacgtg gtcaagacaa tccccagctg ccggatgagc
     5221 accctggaaa ttccagccat ggccttccac atgtttctgt tcggcagcaa gaaaaccgag
     5281 aagctgatcg tggacgtgca caaccggtac aagagactgt tccaggccat gcagaaagaa
     5341 gaagtgaccg ccgagaatat cgccagcttc ggaatcgccg agagcgacct gcctcagaag
     5401 atcctggatc tgatcagcgg caatgcccac ggcaaggatg tggacgcctt catcagactg
     5461 accgtggacg acatgctgac cgacaccgag cggagaatca agagattcaa ggacgaccgg
     5521 aagtccattc ggagcgccga caacaagatg ggaaagagag gcttcaagca gatctccaca
     5581 ggcaagctgg ccgacttcct ggccaaggac atcgtgctgt ttcagcccag cgtgaacgat
     5641 ggcgagaaca agatcaccgg cctgaactac cggatcatgc agagcgccat tgccgtgtac
     5701 gatagcggcg acgattacga ggccaagcag cagttcaagc tgatgttcga gaaggcccgg
     5761 ctgatcggca agggcacaac agagcctcat ccatttctgt acaaggtgtt cgcccgcagc
     5821 atccccgcca atgccgtcga gttctacgag cgctacctga tcgagcggaa gttctacctg
     5881 accggcctgt ccaacgagat caagaaaggc aacagagtgg atgtgccctt catccggcgg
     5941 gaccagaaca agtggaaaac acccgccatg aagaccctgg gcagaatcta cagcgaggat
     6001 ctgcccgtgg aactgcccag acagatgttc gacaatgaga tcaagtccca cctgaagtcc
     6061 ctgccacaga tggaaggcat cgacttcaac aatgccaacg tgacctatct gatcgccgag
     6121 tacatgaaga gagtgctgga cgacgacttc cagaccttct accagtggaa ccgcaactac
     6181 cggtacatgg acatgcttaa gggcgagtac gacagaaagg gctccctgca gcactgcttc
     6241 accagcgtgg aagagagaga aggcctctgg aaagagcggg cctccagaac agagcggtac
     6301 agaaagcagg ccagcaacaa gatccgcagc aaccggcaga tgagaaacgc cagcagcgaa
     6361 gagatcgaga caatcctgga taagcggctg agcaacagcc ggaacgagta ccagaaaagc
     6421 gagaaagtga tccggcgcta cagagtgcag gatgccctgc tgtttctgct ggccaaaaag
     6481 accctgaccg aactggccga tttcgacggc gagaggttca aactgaaaga aatcatgccc
     6541 gacgccgaga agggaatcct gagcgagatc atgcccatga gcttcacctt cgagaaaggc
     6601 ggcaagaagt acaccatcac cagcgagggc atgaagctga agaactacgg cgacttcttt
     6661 gtgctggcta gcgacaagag gatcggcaac ctgctggaac tcgtgggcag cgacatcgtg
     6721 tccaaagagg atatcatgga agagttcaac aaatacgacc agtgcaggcc cgagatcagc
     6781 tccatcgtgt tcaacctgga aaagtgggcc ttcgacacat accccgagct gtctgccaga
     6841 gtggaccggg aagagaaggt ggacttcaag agcatcctga aaatcctgct gaacaacaag
     6901 aacatcaaca aagagcagag cgacatcctg cggaagatcc ggaacgcctt cgatgcaaac
     6961 aattaccccg acaaaggcgt ggtggaaatc aaggccctgc ctgagatcgc catgagcatc
     7021 aagaaggcct ttggggagta cgccatcatg aagggaagcc tgcagtctag agagggagct
     7081 cccaagaaaa agcgcaaggt aactagtgac gcattggacg attttgatct ggatatgctg
     7141 ggaagtgacg ccctcgatga ttttgacctt gacatgcttg gttcggatgc ccttgatgac
     7201 tttgacctcg acatgctcgg cagtgacgcc cttgatgatt tcgacctgga catgctgatt
     7261 aactctagaa gttccggatc tccgaaaaag aaacgcaaag ttggtagcca gtacctgccc
     7321 gacaccgacg accggcaccg gatcgaggaa aagcggaagc ggacctacga gacattcaag
     7381 agcatcatga agaagtcccc cttcagcggc cccaccgacc ctagacctcc acctagaaga
     7441 atcgccgtgc ccagcagatc cagcgccagc gtgccaaaac ctgcccccca gccttacccc
     7501 ttcaccagca gcctgagcac catcaactac gacgagttcc ctaccatggt gttccccagc
     7561 ggccagatct ctcaggcctc tgctctggct ccagcccctc ctcaggtgct gcctcaggct
     7621 cctgctcctg caccagctcc agccatggtg tctgcactgg ctcaggcacc agcacccgtg
     7681 cctgtgctgg ctcctggacc tccacaggct gtggctccac cagcccctaa acctacacag
     7741 gccggcgagg gcacactgtc tgaagctctg ctgcagctgc agttcgacga cgaggatctg
     7801 ggagccctgc tgggaaacag caccgatcct gccgtgttca ccgacctggc cagcgtggac
     7861 aacagcgagt tccagcagct gctgaaccag ggcatccctg tggcccctca caccaccgag
     7921 cccatgctga tggaataccc cgaggccatc acccggctcg tgacaggcgc tcagaggcct
     7981 cctgatccag ctcctgcccc tctgggagca ccaggcctgc ctaatggact gctgtctggc
     8041 gacgaggact tcagctctat cgccgatatg gatttctcag ccttgctggg ctctggcagc
     8101 ggcagccggg attccaggga agggatgttt ttgccgaagc ctgaggccgg ctccgctatt
     8161 agtgacgtgt ttgagggccg cgaggtgtgc cagccaaaac gaatccggcc atttcatcct
     8221 ccaggaagtc catgggccaa ccgcccactc cccgccagcc tcgcaccaac accaaccggt
     8281 ccagtacatg agccagtcgg gtcactgacc ccggcaccag tccctcagcc actggatcca
     8341 gcgcccgcag tgactcccga ggccagtcac ctgttggagg atcccgatga agagacgagc
     8401 caggctgtca aagcccttcg ggagatggcc gatactgtga ttccccagaa ggaagaggct
     8461 gcaatctgtg gccaaatgga cctttcccat ccgcccccaa ggggccatct ggatgagctg
     8521 acaaccacac ttgagtccat gaccgaggat ctgaacctgg actcacccct gaccccggaa
     8581 ttgaacgaga ttctggatac cttcctgaac gacgagtgcc tcttgcatgc catgcatatc
     8641 agcacaggac tgtccatctt cgacacatct ctgtttggat ccgagggcag aggaagtctg
     8701 ctaacatgcg gtgacgtcga ggagaatcct ggcccagaaa tcggtactgg ctttccattc
     8761 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
     8821 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
     8881 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
     8941 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
     9001 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
     9061 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     9121 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     9181 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     9241 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     9301 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     9361 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     9421 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     9481 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     9541 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     9601 tctactctgg agatttccgg ttaatgtaca agtgaaagct taattagctg agcttggact
     9661 cctgttgaaa gggcgaattc tgcagattcg acaatcaacc tctggattac aaaatttgtg
     9721 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     9781 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     9841 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     9901 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     9961 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
    10021 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
    10081 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
    10141 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    10201 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    10261 ccctttgggc cgcctccccg cctggaattc gagctcggta cctttaagac caatgactta
    10321 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
    10381 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
    10441 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10501 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10561 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
    10621 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
    10681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    10741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10801 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
    10861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
    10921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
    10981 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
    11041 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    11101 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    11161 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    11221 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    11281 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    11341 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    11401 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    11461 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    11521 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    11581 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
    11641 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    11701 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11761 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11821 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11881 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    11941 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    12001 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    12061 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    12121 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    12181 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    12241 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    12301 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    12361 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    12421 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    12481 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    12541 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    12601 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    12661 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12721 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12781 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12841 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12901 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    12961 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    13021 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    13081 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    13141 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    13201 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13261 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13321 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13381 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13441 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    13501 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    13561 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    13621 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    13681 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    13741 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    13801 cgcaattaac cctcactaaa gggaacaaaa gctggagctg ca
//
