LOCUS       sequence-392229-        8688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of the gamma-tubulin-binding domain 
            (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous 
            gamma-TuRC from the centrosome. Used in combination with 
            Talpha1-iCre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD...
FEATURES             Location/Qualifiers
     source          1..8688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2260..3282)
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             complement(3289..3315)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3316..3336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4471..4504
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4547..4556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4553..5260
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5607..5624)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5613..5837
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5909..5926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5909..5925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5918..5940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6067..6522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6154..6173)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6364..6385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6548..6652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6653..6721,6722..7513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6871..6890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7684..8272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8173..8192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8426..8443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8560..8581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8596..8601,8602..8619,8620..8626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8634..8650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8639..8661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8658..8674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8658..8674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggc ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt
     2281 ggctcccacg gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca
     2341 tgtcccttcc tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc
     2401 acccaccctg gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc
     2461 ccgtttgttc agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag
     2521 gggcgtggta gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc
     2581 tggatgttct ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg
     2641 ggagagcact ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag
     2701 ggtcactgtg tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat
     2761 tctcatgagg gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg
     2821 ctcgctcctg ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc
     2881 ctgtcctgtc ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag
     2941 actcactgac ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag
     3001 catagcatag tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa
     3061 gtattcgcgt gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca
     3121 tctggcccag ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc
     3181 ctggtcattc ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt
     3241 tcgcaggaat ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg
     3301 aacatcgtat gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct
     3361 tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact
     3421 cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca
     3481 gctcggtcac catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg
     3541 cccagagaag ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc
     3601 tctcccccct tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct
     3661 cgcaaaagca actagccgag catccattat ggggtggagc tggggagggg tagagaggga
     3721 agaaagaaaa cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat
     3781 aatttaaggt tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa
     3841 ttccagcttt tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct
     3901 ctgttcgctt taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta
     3961 aatgtttagt tttacagttg tattattgtg gttattatta ttactattat tattaatatt
     4021 tgagcaaaat tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg
     4081 ggaggcagag gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat
     4141 gcctgtaaat cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat
     4201 atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa
     4261 ggaaaaaaga gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc
     4321 tttagcggta gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc
     4381 accccagagc ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga
     4441 tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4501 ttattaggtc cctcgacctg cagcctccgc agcccccaag ctagcagcca ccatggtgag
     4561 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcgcat
     4621 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4681 cgagggcttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4741 ggacatcctg tcccctcatt tcacctacgg ctccaaggcc tacgtgaagc accccgccga
     4801 catccccgac tacttcaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4861 ctacgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4921 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg tgatgcagaa
     4981 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gtgccctgaa
     5041 gggcaagatc aagatgaggc tgaagctgaa ggacggcggc cactacacct ccgaggtcaa
     5101 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacatcg tcgacatcaa
     5161 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     5221 gggccgccac tccaccggcg gcatggacga gctgtacaag tactcagatc tcgagctcaa
     5281 gcttcgaatt ctagccgaca gcattggaaa taaccggcaa aatgcaccat tgacttccat
     5341 tcaaattcgt tttattcaga acatgataca ggaaacgttg gatgacttta gagaagcatg
     5401 ccatagggac attgtgaatt tgcaagtgga gatgattaaa cagtttcata tgcaactgaa
     5461 tgaaatgcat tctttgctgg aaagatactc agtgaatgaa ggtttagtgg ctgaaattga
     5521 aagactacga gaagaaaaca aaagattacg ggcccacttt tgaggatcca ccggatctag
     5581 ataagtaatg agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5641 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5701 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5761 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5821 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga gatccactag
     5881 ttctagcctc gaggctagag cggccgccac tggccgtcgt tttacaacgt cgtgactggg
     5941 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     6001 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     6061 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6121 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6181 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6241 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6481 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6541 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6601 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6661 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6721 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6781 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6841 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6901 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6961 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7021 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7081 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7141 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7201 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7261 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7321 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7381 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7441 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7501 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7561 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7621 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     8041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     8101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     8161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     8221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     8281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8341 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8401 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8461 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8521 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8581 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8641 cggataacaa tttcacacag gaaacagcta tgaccatgag gcgcgccg
//
