LOCUS       sequence-392230-       11465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of Calmodulin Regulated Spectrin 
            Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. 
            Used in combination with Talpha1-iCre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3
FEATURES             Location/Qualifiers
     source          1..11465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2260..3282)
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             complement(3289..3315)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3316..3336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4471..4504
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4532..4558
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4562..4588
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8384..8401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8390..8614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8686..8703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8686..8702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8695..8717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8844..9299
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8931..8950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9141..9162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9325..9429
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9430..9498,9499..10290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9648..9667)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10461..11049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10950..10969
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11203..11220
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11337..11358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11373..11378,11379..11396,11397..11403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11411..11427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11416..11438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11435..11451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11435..11451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcggr grggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gsgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt
     2281 ggctcccacg gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca
     2341 tgtcccttcc tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc
     2401 acccaccctg gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc
     2461 ccgtttgttc agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag
     2521 gggcgtggta gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc
     2581 tggatgttct ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg
     2641 ggagagcact ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag
     2701 ggtcactgtg tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat
     2761 tctcatgagg gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg
     2821 ctcgctcctg ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc
     2881 ctgtcctgtc ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag
     2941 actcactgac ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag
     3001 catagcatag tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa
     3061 gtattcgcgt gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca
     3121 tctggcccag ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc
     3181 ctggtcattc ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt
     3241 tcgcaggaat ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg
     3301 aacatcgtat gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct
     3361 tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact
     3421 cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca
     3481 gctcggtcac catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg
     3541 cccagagaag ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc
     3601 tctcccccct tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct
     3661 cgcaaaagca actagccgag catccattat ggggtggagc tggggagggg tagagaggga
     3721 agaaagaaaa cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat
     3781 aatttaaggt tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa
     3841 ttccagcttt tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct
     3901 ctgttcgctt taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta
     3961 aatgtttagt tttacagttg tattattgtg gttattatta ttactattat tattaatatt
     4021 tgagcaaaat tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg
     4081 ggaggcagag gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat
     4141 gcctgtaaat cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat
     4201 atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa
     4261 ggaaaaaaga gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc
     4321 tttagcggta gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc
     4381 accccagagc ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga
     4441 tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4501 ttattaggtc cctcgacctg cagccaccat gtacccatac gatgttccag attacgctgg
     4561 ctatccctat gacgtcccgg actatgcagg aagttcagga ggttctagta tggtggaagc
     4621 ggcgcccgca gggtccgggc cactgcggag gactttcctg gtccccgaga tcaagtcttt
     4681 ggaccagtac gatttctctc gagccaaggc ggcggcgagt ctggcgtggg tgctgcgggc
     4741 cgcattcggg ggagcagagc atgtgcctcc ggagctgtgg gaacccttct acaccgatca
     4801 gtacgcacag gagcacgtga agcccccagt gacgcggctg ctgctctctg cggaactata
     4861 ctgtcgggcc tggcgccagg cactgccaca gctcgagcca tcccccagtc cctctgctct
     4921 gttggccttg ctggcgagga ggggcacggt gccttcactg cctgagcacc cagtgcgcga
     4981 ggctgacctg aaacaccagc caattctcat gggagcccac ctagctgtca tcgatgctct
     5041 catggttgcc ttctcatttg agtggacaaa gacactacct ggtcccttgg ctctgagcag
     5101 cttggagcac aaactccttt tctgggtaga cacaactgtt cggcggctgc aggagaagac
     5161 agaacaagaa gcagcccagc gtgcatctcc tgcagcccct ctggatgggg cctctcctgc
     5221 ccagccctcg attcgatatc gcaaggaccg tgccattgcc cgaagggctc cctgctttcc
     5281 aaatgtgact acccttcagg acctggccag tggggcagca ctggctgcca ccatccactg
     5341 ctattgtccc cagctattac gacttgagga ggtgtgcctc aaggacccca tgtctgtggc
     5401 agacagtcta tacaacctcc agctggtgca agacttctgt gcctcccatc ttcctcgagg
     5461 ctgccccctg tcccttgaag acttgctgta tgtcccacca cccctcaagg tcaacctggt
     5521 ggtgctgttg gctgagatgt atatgtgctt tgaggttctg aagcctgatt ttgtgcaggc
     5581 caaagacttg cctgatggac atgctgtctc cccccggaat acagagactg ttccatctca
     5641 gaacaacagt ggcagcagtt ctcctgtctt caacttccgt cacccacttc tgtcacctgg
     5701 tggtccccag tctccactcc gaggatccac aggctccctg aagtcctctc catcaatgtc
     5761 tcacatggag gctcttggca aagcctggaa ccgtcagctt agccgtcccc tctcccaggc
     5821 tgtgtcgttc agcactccct ttggcctgga cagcgatgtg gatgtcgtca tgggagatcc
     5881 tgtcctgctc cgctccgtca gctcagacag tctgggtcct ccacgtcctg tgtcaacatc
     5941 atcccggaat tctgctcagc cagccccaga atctggagac ctacccacga ttgaagaggc
     6001 cctgcagatc attcacagtg ctgagccccg actgctccct gatggggctg ctgatggcag
     6061 tttctacctc cattctcctg agggtctctc caaaccacca ctctccccct accctcccga
     6121 aggagcctca aagccgctgt ctgataggct caacaaggcg cctatctata tatcacaccc
     6181 tgagaaccct tcaaaatcat ctccctgctc aacaggagag atactgaaac caccaccccc
     6241 atccgagggt tcccccaaag ctgtggcttc atccccagca gccaacaact ccgaagtgaa
     6301 gatgaccagt tttgctgaac gcaagaaaca gctggtgaag gctgaggctg agtcaggatt
     6361 ggggtctcca acatccaccc ccgtagcacc tgaggccttg agctcagaga tgagtgagct
     6421 gggagccagg ctggaggaga agcgccgagc catagaggca cagaaacgac gcattgaggc
     6481 aatctttgcc aagcataggc agaggctggg caagagcgct ttcctgcagg tgcagcctcg
     6541 ggaggctgca ggggaggccg aggaggaagc tgagctgggc tcagttcctg gtggggaacg
     6601 gccagcaggt gagggccagg gtgagccgtc cttacggcac aagtcagtta ccttctctcc
     6661 agacctgggc ccagtgcccc cagagggact tggggattac aatagagcag tcagtaagct
     6721 gagtgccgct ctgagctctc tgcagcggga catgcagagg ctcacagacc agcaacagcg
     6781 gcttctagcc cctccagaag ctcctggacc tgccccacca cctgcagcct gggtcattcc
     6841 tggacccgcc actgggccta aagcagcatc ccccagccct gcccgtcgtg ccccagctgc
     6901 ccgacgcagc cctgggccag gccccagccc aacaccccgt agtccaaaac atgcaaggcc
     6961 ggcagagctg aagcttgcac ctttgacaag ggtactcaca ccaccccatg atgtagacag
     7021 cctccctcac ctacgcaagt tttcacccag ccaggtgcct gtacagactc gctcctctat
     7081 cctcctgtcg gaggggacac ctcccgagga gcccaccacc aagcctgccc tcattgagat
     7141 ccctctagcc agcctggggg agcctgctgc tgatgaggaa ggagatggga gcccccctgg
     7201 ggctgaggat tccttagagg aagaggcatc ttctgaggga gagccccgat cagggcttgg
     7261 attcttttat aaggacgaag acaagcctga ggatgagatg gctcagaagc gagctagcct
     7321 gctggagcgt cagcagaggc gggtagagga agcccggcgg cgcaaacagt ggcaggaggc
     7381 agagaaggag cagaaacggg aggaggccgc caggctggct caggaggctc caggcttggc
     7441 ctttacaact cctgttgtag cctctgcggc tccagtggcc accttggctc ctactaccag
     7501 agccatggcc ccagctgagg aggaggtggg cccccggcgg ggggacttca caagacttga
     7561 gtatgaacgc cgggcacaac tgaaactgat ggatgacctt gataaggtgc tacggccccg
     7621 ggcctcaggg accgggggac cagggcgggg cgggcgcagg gccacccggc cacgctctgg
     7681 ttgctgtgat gactcggcct tggcacgaag cccagcccgc ggcctgctgg gttcacggct
     7741 cagcaaggtc tattcccagt ccacgctgtc cctatctacg gtggccaacg aggctcccaa
     7801 taaccttggt gtgaagaggc ccacctctcg ggccccttct ccatcaggcc tcatgtctcc
     7861 aagccgcctg cctggcagtc gagaacgtga ctgggagaat ggaagcaatg catcctcccc
     7921 agcatcagtg cctgagtaca caggtccccg gctatacaag gaacccagcg ccaagtctaa
     7981 caagtttatc atccacaatg ccttgtcaca ctgctgcctg gcaggcaagg tgaatgagcc
     8041 ccagaagaac agaattctag aggaaatcga gaaaagcaag gccaaccact tcctgattct
     8101 ctttcgggac tcaagctgcc agttccgggc cctctacact ctgtctgggg agacagagga
     8161 gctatcgagg ctggcaggct atgggcctcg gaccgtcact cctgccatgg tcgaaggcat
     8221 ctataagtac aactcggatc gcaaacggtt cacccagatc cctgctaaaa ccatgtctat
     8281 gagtgtagac gccttcacta tccagggaca cctttggcaa agcaagaagc ccaccacgcc
     8341 caagaagggt ggcggtaccc ccaaataaga gctcgctgat cagcctcgac tgtgccttct
     8401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8641 ggctcgagat ccactagttc tagcctcgag gctagagcgg ccgccactgg ccgtcgtttt
     8701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     8761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     8821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     9301 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9361 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9421 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9481 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9541 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9601 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9661 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9721 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9781 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9841 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9901 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9961 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    10021 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    10081 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10141 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    10201 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10261 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10321 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10381 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10441 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10501 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10561 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    10621 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10681 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10741 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10801 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10861 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10921 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10981 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    11041 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    11101 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    11161 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    11221 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    11281 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    11341 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    11401 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgaggcg
    11461 cgccg
//
