LOCUS       sequence-392231-       10406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in
            combination with Talpha1-iCre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA
FEATURES             Location/Qualifiers
     source          1..10406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2260..3282)
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             complement(3289..3315)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3316..3336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4471..4504
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7255..7281
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7285..7311
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7325..7342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7331..7555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7627..7644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7627..7643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7636..7658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7785..8240
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7872..7891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8082..8103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8266..8370
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8371..8439,8440..9231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8589..8608)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9402..9990
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9891..9910
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10144..10161
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10278..10299
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10314..10319,10320..10337,10338..10344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10352..10368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10357..10379
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10376..10392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10376..10392
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggcggr gaggkgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt
     2281 ggctcccacg gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca
     2341 tgtcccttcc tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc
     2401 acccaccctg gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc
     2461 ccgtttgttc agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag
     2521 gggcgtggta gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc
     2581 tggatgttct ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg
     2641 ggagagcact ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag
     2701 ggtcactgtg tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat
     2761 tctcatgagg gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg
     2821 ctcgctcctg ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc
     2881 ctgtcctgtc ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag
     2941 actcactgac ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag
     3001 catagcatag tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa
     3061 gtattcgcgt gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca
     3121 tctggcccag ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc
     3181 ctggtcattc ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt
     3241 tcgcaggaat ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg
     3301 aacatcgtat gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct
     3361 tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact
     3421 cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca
     3481 gctcggtcac catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg
     3541 cccagagaag ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc
     3601 tctcccccct tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct
     3661 cgcaaaagca actagccgag catccattat ggggtggagc tggggagggg tagagaggga
     3721 agaaagaaaa cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat
     3781 aatttaaggt tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa
     3841 ttccagcttt tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct
     3901 ctgttcgctt taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta
     3961 aatgtttagt tttacagttg tattattgtg gttattatta ttactattat tattaatatt
     4021 tgagcaaaat tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg
     4081 ggaggcagag gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat
     4141 gcctgtaaat cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat
     4201 atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa
     4261 ggaaaaaaga gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc
     4321 tttagcggta gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc
     4381 accccagagc ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga
     4441 tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4501 ttattaggtc cctcgaccct gcaggccacc atgtgccgga tagcgggagc gccgcggacc
     4561 ctgctgccgc ttctggcggc cttgcttcag gcgtctgtgg aggcttctgg tgaaattgca
     4621 ttatgcaaga ctggatttcc tgaagatgtt tacagcgcag tcttaccgaa ggatgtgcac
     4681 gaaggacagc cccttctcaa tgtgaaattc agcaactgca atagaaaaag gaaagttcag
     4741 tatgaaagca gcgagccagc agatttcaag gtggacgagg acggcacggt gtatgctgtg
     4801 agaagcttcc ctctcactgc agagcaggca aagttcctga tatatgccca agacaaagaa
     4861 acccaggaaa agtggcaggt agctgtaaac ctgagccggg agccaaccct gactgaggag
     4921 cctatgaagg aaccacatga aattgaagaa atagtattcc ctagacaact tgccaagcac
     4981 agtggagctc tacaaaggca gaagagagac tgggtcatcc cgccaatcaa cttgccagaa
     5041 aactccagag gaccctttcc tcaagagctt gtcagaatca ggtctgatag agataaaaac
     5101 ctttccctga gatacagcgt cactgggcca ggagctgacc agcctccaac gggcatcttc
     5161 attatcaacc ccatctcagg acagctgtca gtcacaaagc ctctggatcg agagctgata
     5221 gcccggtttc acttgagagc acatgcagtg gacatcaatg gcaatcaagt ggagaacccc
     5281 attgacattg tcatcaatgt tattgacatg aatgataaca gacctgagtt tctgcaccag
     5341 gtttggaatg ggtctgttcc agagggatca aagcctggga cgtatgtgat gacggtcact
     5401 gccattgatg cggatgatcc aaatgccctg aatggaatgc tgcggtacag gatcctgtcc
     5461 caggcgccca gcacaccttc acccaacatg tttacaatca acaatgagac tggggacatc
     5521 atcactgtgg cagctggtct ggatcgagag aaagtgcaac agtatacgtt aataattcaa
     5581 gccacagaca tggaaggcaa tcccacttat ggcctttcaa acacagccac agccgtcatc
     5641 acggtgacag atgtcaatga caatcctcca gagtttactg ccatgacttt ctacggagaa
     5701 gtccctgaga acagggtgga cgtcattgta gccaacctaa ctgtcacgga caaagatcag
     5761 ccccacacgc cggcctggaa tgcggcatac agaatcagtg gtggagaccc tacaggaagg
     5821 tttgccatcc tgacagaccc caacagcaat gatgggctag tcacagtggt aaaaccaatt
     5881 gactttgaaa cgaataggat gtttgtcctt actgttgctg cagaaaacca agtgccatta
     5941 gctaaaggca ttcagcaccc acctcagtcg acagccactg tgtctgtgac agttattgat
     6001 gtcaatgaaa atccttattt tgccccaaat cctaaaatca ttcgccaaga ggaaggcctc
     6061 cacgcaggta ccatgctgac cacgctcact gctcaggacc ccgatcgata tatgcaacag
     6121 aatatcagat acacaaaatt gtctgatcct gccaactggc tgaaaataga ccccgtgaat
     6181 gggcagatca ctactattgc cgttttggac agagaatcgc caaatgtaaa aaacaacatc
     6241 tataatgcta ccttccttgc ttctgacaat ggaatcccgc ctatgagtgg gacaggaaca
     6301 ctgcaaatct atttacttga tatcaatgac aacgcccctc aggtgttacc tcaagaggca
     6361 gagacctgtg aaactccaga acccaactca attaacatca cagcacttga ttatgacata
     6421 gacccaaacg ccgggccatt cgcgtttgat cttcccttat ctccagtgac tattaaaaga
     6481 aactggacca tcaaccggct taatggtgat tttgctcagc tcaatttaaa gataaaattt
     6541 ttggaagctg gtatctatga agttcccatc attatcacag attcagggaa tccccccaag
     6601 tccaacattt ccatcctgcg tgtgaaagtt tgccagtgtg actccaatgg agactgcacg
     6661 gacgtggaca ggatcgtggg tgcagggctt ggcacgggtg ccatcatcgc tatccttctg
     6721 tgtatcatca tcctgctgat ccttgttctc atgtttgtgg tatggatgaa acggcgggat
     6781 aaagagcgcc aagccaagca gcttttaatt gacccagaag atgatgtaag agataatata
     6841 ttgaaatatg atgaagaagg tggaggagaa gaagaccagg actatgactt gagccagctc
     6901 cagcaaccag atactgtgga gcctgatgcc atcaagcccg tgggaatcag acggctagac
     6961 gagaggccta tccatgctga gccacagtac ccagtccgat ccgcagcccc acaccctggg
     7021 gatattgggg acttcattaa tgagggcctt aaagctgctg acaacgaccc cacggcgcca
     7081 ccgtatgact ccctcttagt ctttgactac gagggcagcg gctccacggc tggctccttg
     7141 agctccctca actcctccag tagcggtggg gaccaggact atgactacct gaatgactgg
     7201 ggaccccgct tcaagaaact ggcggacatg tacggcggtg gtgacgacga gctctaccca
     7261 tacgatgttc cagattacgc tggctatccc tatgacgtcc cggactatgc atgacgctga
     7321 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7441 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7501 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7561 gaggcggaaa gaaccagctg gggctcgaga tccactagtt ctagcctcga ggctagagcg
     7621 gccgccactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7681 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7741 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7801 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7861 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7921 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7981 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8041 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8101 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8161 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8221 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8281 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8341 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8401 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8461 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8521 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8581 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8641 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8701 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8761 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8821 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8881 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8941 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9001 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9061 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9121 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9181 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9241 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9301 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10261 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10321 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10381 aacagctatg accatgaggc gcgccg
//
