LOCUS       sequence-392247-       10800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Elongation reporter construct to quantify elongation of LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Guzikowski AR, Harvey AT, Zhang J, Zhu S, Begovich K, Cohn MH, 
            Wilhelm JE, Zid BM
  TITLE     Differential translation elongation directs protein synthesis in 
            response to acute glucose deprivation in yeast.
  JOURNAL   RNA Biol. 2022;19(1):636-649. doi: 10.1080/15476286.2022.2065784. 
            Epub 2021 Dec 31.
  PUBMED    35491906
REFERENCE   2  (bases 1 to 10800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA Biol.";
            date: "2022"; volume: "19(1):636-649. doi"; pages: " 
            10.1080/15476286.2022.2065784. Epub 2021 Dec 31"
COMMENT     Sequence Label: TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)
FEATURES             Location/Qualifiers
     source          1..10800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(664..1758)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1759..2166)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2467..2922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2604..2625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2816..2835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3063..3079
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3086..3105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3086..3104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3139..3326
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     protein_bind    join(3384..3402,3403..3425,3426..3444,3445..3467,3468..3486,
                     3487..3509,3510..3528,3529..3551,3552..3570,3571..3593,
                     3594..3612,3613..3635,3636..3654)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             3878..6949
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(3898..3914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7490..7609
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     primer_bind     7619..7635
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7619..7635
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_RNA        7660..7678
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7699..7717
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7769..7787
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7808..7826
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7878..7896
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7917..7935
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        7987..8005
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8026..8044
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8096..8114
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8135..8153
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8205..8223
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        8244..8262
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      8302..8489
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8495..8511)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8496..8512)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8540..8558)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8579..8595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8579..8595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8592..8614)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8603..8619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8627..8633,8634..8651,8652..8657))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8672..8693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8810..8827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8981..9569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9061..9080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9740..10531,10532..10600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10363..10382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10601..10705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10773..10791
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
      241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
      301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
      361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
      421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
      481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
      541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
      601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
      661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
      721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
      781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
      841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
      901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
      961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
     1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
     1081 agaatcaatc aattgatgtt gaaccttcaa tgtagggaat tcgttcttga tggtttcctc
     1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
     1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
     1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
     1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
     1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
     1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
     1501 actaccggta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
     1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag cagcaccacc
     1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
     1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
     1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
     1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
     1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
     1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
     1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
     2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
     2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
     2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
     2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
     2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
     2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
     2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     2941 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3001 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3061 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
     3121 ccgcccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt
     3181 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     3241 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc
     3301 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc
     3361 aattcctcga tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     3541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     3601 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3661 aaagtcgagc tcggtaccct atggcatgca tgtgctctgt atgtatataa aactcttgtt
     3721 ttcttctttt ctctaaatat tctttcctta tacattaggt cctttgtagc ataaatcaac
     3781 aaatattaac tcaattatta ttatttataa ttacaaaaac aaaacaacaa gtttgagact
     3841 ttaatatctt ttgattacta aaaacaacaa atttcaaatg accatgatta cggattcact
     3901 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3961 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4021 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
     4081 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     4141 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
     4201 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     4261 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
     4321 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
     4381 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
     4441 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
     4501 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
     4561 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
     4621 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
     4681 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
     4741 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa
     4801 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc
     4861 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
     4921 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
     4981 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
     5041 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct
     5101 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
     5161 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
     5221 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa
     5281 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     5341 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     5401 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     5461 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
     5521 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt
     5581 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc
     5641 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt
     5701 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt
     5761 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
     5821 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
     5881 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
     5941 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
     6001 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
     6061 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     6121 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     6181 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     6241 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     6301 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
     6361 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa
     6421 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
     6481 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca
     6541 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc
     6601 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
     6661 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
     6721 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact
     6781 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
     6841 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt
     6901 acagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaag gtgacggtgc
     6961 tggtttaatt aacatggttt ttactttaga agattttgtt ggtgattggc gtcaaactgc
     7021 tggttataat ttagatcaag ttttagaaca aggtggtgtt tcttctttat ttcaaaattt
     7081 aggtgtttct gttactccta ttcaacgtat tgttttatct ggtgaaaatg gtttaaaaat
     7141 tgatattcat gttattattc cttatgaagg tttatctggt gatcaaatgg gtcaaattga
     7201 aaaaattttt aaagttgttt atcctgttga tgatcatcat tttaaagtta ttttacatta
     7261 tggtacttta gttattgatg gtgttactcc taatatgatt gattattttg gtcgtcctta
     7321 tgaaggtatt gctgtttttg atggtaaaaa aattactgtt actggtactt tatggaatgg
     7381 taataaaatt attgatgaac gtttaattaa tcctgatggt tctttattat ttcgtgttac
     7441 tattaatggt gttactggtt ggcgtttatg tgaacgtatt ttagctaatt ctcatggttt
     7501 tcctcctgaa gttgaagaac aagctgctgg tactttacct atgtcttgtg ctcaagaatc
     7561 tggtatggat cgtcatcctg ctgcttgtgc ttctgctcgt attaatgttt aaggccgctc
     7621 tagaactagt ggatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct
     7681 gcaggtcgac tctagaaaac atgaggatca cccatgtctg cagtattccc gggttcatta
     7741 gatcctaagg tacctaattg cctagaaaac atgaggatca cccatgtctg caggtcgact
     7801 ctagaaaaca tgaggatcac ccatgtctgc agtattcccg ggttcattag atcctaaggt
     7861 acctaattgc ctagaaaaca tgaggatcac ccatgtctgc aggtcgactc cagaaaacat
     7921 gaggatcacc catgtctgca gtattcccgg gttcattaga tcctaaggta cctaattgcc
     7981 tagaaaacat gaggatcacc catgtctgca ggtcgactct agaaaacatg aggatcaccc
     8041 atgtctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaacatg
     8101 aggatcaccc atgtctgcag gtcgactcta gaaaacatga ggatcaccca tgtctgcagt
     8161 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaacatga ggatcaccca
     8221 tgtctgcagg tcgactccag aaaacatgag gatcacccat gtctgcagta ttcccgggtt
     8281 cattagatct cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     8341 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     8401 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag
     8461 gtcgctctta ttgaccacac ctctacaagc ttatcgatac cgtcgacctc gagggggggc
     8521 ccggtaccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt
     8581 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8641 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8701 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8761 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    10741 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
