LOCUS       sequence-392311-        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LOV-Turbo on the mammalian outer mitochondrial membrane, 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Lee SY, Cheah JS, Zhao B, Xu C, Roh H, Kim CK, Cho KF, Udeshi ND, 
            Carr SA, Ting AY
  TITLE     Engineered allostery in light-regulated LOV-Turbo enables precise 
            spatiotemporal control of proximity labeling in living cells.
  JOURNAL   Nat Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5.
  PUBMED    37188954
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5."
COMMENT     Sequence Label: OMM-V5-LOV-Turbo
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2509..2550
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3935..4523
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3988..4008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4406..4417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4543..5053
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4972..4990
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5063..5806
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5816..5872
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5873..6469
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6366..6386
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6568..6801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6873..6994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6910..6929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6964..6983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7034..7169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7169..7240
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7241..7309,7310..8101)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7459..7478)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8272..8860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8761..8780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9014..9031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9148..9169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9184..9189,9190..9207,9208..9214)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9222..9238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9227..9249
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9246..9262
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9246..9262
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9281..9301
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9283..9301
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagcgcc accatggcca tccagctgcg cagcctgttc
     2341 cccctggccc tgcccggcct gctggccctg ctgggctggt ggtggttctt cagccgcaag
     2401 aaggacctgg agctgaagct gcgcatcctg cagagcaccg tgccccgcgc ccgcgaccct
     2461 cccgtggcca ccggttccag tggaggcggt agctcaggtt cgggatccgg caagcccatc
     2521 cccaaccccc tgctgggcct ggacagcacc ggtaccaaag acaacacagt gcctctgaag
     2581 ctgatcgccc tcctggccaa tggagagttc cacagcggcg agcagctggg cgaaaccctg
     2641 ggcatgagca gagccgctat caacaagcac atccagaccc ttcgcgactg gggcgtcgac
     2701 gtgttcagcg tgcccggcaa gggctacagc ctgcctgagc ctatccccct gctgaacgcc
     2761 aagcagattc agggccagct ggaatttaga gccaccaccc tggaaagaat cgagaagtct
     2821 tttgtgatca ccgaccccag actgcctgat aatcctatca tcttcgtgtc tgatagcttc
     2881 ctgcagctca ccgagtattc tcgggaagag atcctcggaa gaaattgcag attcctgcag
     2941 ggcccagaga cagatagagc cacagtgcgg aagatcagag atgccatcga caaccagaca
     3001 gaggtgaccg tgcagctgat caactacacc aagtccggca aaaagttctg gaacgttttc
     3061 catctccaac ctatgagaga ctacaagggc gacgtgcagt acttcatcgg agttcagctg
     3121 gacggcaccg agagactgca cggcgccgca gagcgggaag ctgtgtgcct ggtcaagaag
     3181 accgccttcc agatcgccgt ggctgtgctg cccgtggtgg acagcacaaa ccagtacctg
     3241 ctggacagaa tcggcgagct gaagtccggc gacgcttgta tcgccgaata ccagcaggcc
     3301 ggcagaggca gcagaggccg gaaatggttc agccccttcg gcgccaacct gtacctgagc
     3361 atgttttggc ggctgaaaag aggccctgcc gccacaggcc tgggccccgt gataggaatc
     3421 gtgatggccg aggctctgag gaagctgggt gtggacaaag tgcgggtgaa gtggcctaac
     3481 gacctgtacc tgcaggacag aaagctggcc ggcatcctgg tggaactggc tggcatcacc
     3541 ggcgacgccg ctcagattgt gatcggcgct ggcatcaatg tggccatgag acgcgtggaa
     3601 gagagcgtgg tcaaccaggg ctggatcaca ctgcaagagg ccggtgtgaa cctggatagg
     3661 aacaccctgg ccgccaccct gatcagagag ctgagagccg ctctggaatt gtttgagcag
     3721 gagggcctgg ccccttacct gccacggtgg gagaaactgg ataacttcat caacaggcct
     3781 gtgaagctga tcatcggcga taaggaaatc ctgggaatca gccggggcat cgacaagcag
     3841 ggagccctgc tgctggaaca agatggagtg attaagcctt ggatgggcgg agaaatcagc
     3901 ctgcggtccg ccgagaagta atagggccga attcaatcaa cctctggatt acaaaatttg
     3961 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4021 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4081 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4141 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4201 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4261 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4321 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4381 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4441 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4501 ctccctttgg gccgcctccc cgcctgtctg cagatatcat ccggggttgg ggttgcgcct
     4561 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4621 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4681 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4741 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4801 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4861 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4921 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4981 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     5041 cctctctccc cagcaattca ccatgtctag actggacaag agcaaagtca taaactctgc
     5101 tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     5161 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5221 tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga
     5281 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5341 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5401 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5461 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5521 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc
     5581 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5641 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     5701 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     5761 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggggaa gcggagctac
     5821 taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac ctaccgagta
     5881 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     5941 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     6001 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     6061 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     6121 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     6181 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     6241 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     6301 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     6361 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6421 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaac tgtttcgcct
     6481 cggcgtccgg actagaggta cctttaagac caatgactta caaggcagct gtagatctta
     6541 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6601 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6661 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6721 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6781 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     6841 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     6901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7141 gaagtagtga ggaggctttt ttggaggcct ttcaaatatg tatccgctca tgagacaata
     7201 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7261 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7321 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7381 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7441 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7501 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7561 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7621 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7681 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7741 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7801 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7861 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7921 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7981 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8041 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8101 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8161 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8221 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8281 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8341 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8401 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8461 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8521 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8581 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8641 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8701 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8761 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8821 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8881 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8941 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9001 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9061 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9121 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9181 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9241 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     9301 ggaacaaaag ctggagctgc aagc
//
