LOCUS       sequence-392316-        9469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2750
FEATURES             Location/Qualifiers
     source          1..9469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..63)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(43..61)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(82..98)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(82..98)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(95..117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(130..136,137..154,155..160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    175..196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(313..330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(484..1072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(564..583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1243..2034,2035..2103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1866..1885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2104..2208)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2234..2689)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2371..2392)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2583..2602
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2816..2838
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2830..2847
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2831..2847
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2857..2876
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2857..2875
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2896..3031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3071..3192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3082..3101)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3136..3155
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3270..3503
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3575..4163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3681..3692
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4090..4110
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(5391..6569)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5400..6338
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5423..5443)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6707..6824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6867..7469)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(6953..6973)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7450..7469
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7501..7697)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(7509..7719)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7515..7650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(7950..7994)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8179..8412
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8905..9030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9077..9257
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(join(9258..9469,1..15))
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt gagggttaat
       61 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
      241 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      841 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      901 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      961 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1021 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1081 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     1261 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1321 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1381 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1441 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1501 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1561 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1621 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1681 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1741 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1801 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1861 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1921 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1981 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     2041 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     2101 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     2161 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     2221 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2281 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2341 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2401 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2461 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2521 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2581 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2641 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
     2701 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2761 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2821 tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa
     2881 ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg
     2941 ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat
     3001 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg atagctagag
     3061 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3121 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3181 aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat
     3241 aataagatga catgaactac tatacgtact gctagagatt ttccacactg actaaaaggg
     3301 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     3361 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     3421 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg
     3481 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     3541 agctgccttg taagtcattg gtcttaaagt cgacgcgggg aggcggccca aagggagatc
     3601 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
     3661 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
     3721 agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac
     3781 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     3841 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     3901 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     3961 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     4021 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     4081 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     4141 attttgtaat ccagaggttg attgtcgact taacgcgtaa gatgaattct ccttcccaac
     4201 tatcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggac
     4261 tagttcatta ctaggatgag ctctccggct cggacgcgtg cagtaggagg ccgccgccgc
     4321 cgccgctgct ggacttgtgc ttcttcaaca gctgcgtgat tttctcgtcg tccgagttgg
     4381 gatccagagg cttattgtag tcgtcgtcct cttcctcgtt ctccgaggcc cccttgaggc
     4441 gctctgtctc cgagtcctgc ttcttcttgg ccgtggccat ctcggcagcg tgcttcttcc
     4501 tccacttggt ccggcggttc tggaaccaga ccttgacctg actctctgtc atccccaacg
     4561 aataggccaa acgagccctc tcgggccccg ccaagtattt tgtttgttcg aaagtcttct
     4621 ccagggcgaa gatctgctgt ccggaaaaag tgggtctcgt gtgttttctc ttcccgtctt
     4681 tgtccaacaa aatggatcct tgatgagggg tacaggccag gcgtgcgtcc ctccagggcg
     4741 ggctctgcat cactccgggc cagaagatgg gcgtccggcc aggcagctca gccagcggct
     4801 tggggtaccg gcccacggcg gccacggccg cggcgctggg gctgaagtag agcccgggcg
     4861 gcggcggcgg cgggctcagg ctgctaaagc gtggcagtcc ggccagcagc cccgccgggg
     4921 atgaggcggc ggctgcggcc gcggcagcag ccgcggcggc ggcagaggcg gaggaggcag
     4981 aggcggacga ggaagaggag gaggaggaac cggagggcga ggcggagggc agggcggccc
     5041 ccgaggccac gggcatggag ggccggctca ggatatcgtt gatgccgtgt ggggtggcgg
     5101 ccgagagctg ctgcgggggg ctgccgaggg atgagagccc ccccgtggcc gggggcttca
     5161 ggccgcctgg gttgtgggtg cccagaggcg gggagggcga cgaggaggac gacgacgagg
     5221 acgaggagga gggggggccg gcaggcagcg ggggatacgc ggcagggtac agcggggtct
     5281 tcatctcggc catgctgtgc agggcggcca ggggagggct gctgagcagg aatgcgctct
     5341 gccgggtgcc ctccattgcc cccaccgcta acatggtggc ggtgctagcc tcacgacacc
     5401 tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca
     5461 aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca
     5521 gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa
     5581 gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc
     5641 gtggagtcac atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac
     5701 tcacccgccc gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg
     5761 ggaagcggcc atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag
     5821 ggcggggcga tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac
     5881 tacccccgtc cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc
     5941 tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga
     6001 tcttggcccg catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa
     6061 aaattttaaa tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag
     6121 attcgcacgc ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg
     6181 gctccttaag cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca
     6241 agaatcacgt actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta
     6301 aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg
     6361 ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg
     6421 ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc
     6481 taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg
     6541 cgctctgccc actgacgggc accggagcca attcccactc ctttcaagac ctagaaggtc
     6601 cattagctgc aaagattcct ctctgtttaa aactttatcc atctttgcac ctgcaggcct
     6661 cgagccggcg ccaaagtgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     6721 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     6781 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg
     6841 ccatttgtct caagatctag aattcgttaa ctggcaccgg gcttgcgggt catgcaccag
     6901 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
     6961 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
     7021 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     7081 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     7141 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     7201 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc cacggcggcg
     7261 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     7321 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     7381 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     7441 aggcgcaccg tgggcttgta ctcggtcatg gtaagctccg gtggtacctc tagaaagctt
     7501 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     7561 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     7621 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     7681 tgactaattg agacgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     7741 acacctggtt gctgactaat tgagatgcaa tgcatgggtc ccctcggggt tgggaggtgg
     7801 gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga aagtacagca
     7861 aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt tatataccac
     7921 agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat
     7981 tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt
     8041 gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc
     8101 agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca
     8161 gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt
     8221 cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca
     8281 atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt
     8341 accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg
     8401 aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg
     8461 gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc
     8521 caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt
     8581 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     8641 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     8701 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     8761 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     8821 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     8881 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     8941 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     9001 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     9061 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     9121 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     9181 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     9241 tctaaccaga gagacccgtt tatgtatcga gctaggcact taaatacaat atctctgcaa
     9301 tgcggcaatt cagtggttcg tccaatccat gtcagacccg tctgttgcct tcctaataag
     9361 gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt ctctccttgt
     9421 aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagagta
//
