LOCUS       sequence-392319-        9174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LOV-Turbo in the mammalian cytosol, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Lee SY, Cheah JS, Zhao B, Xu C, Roh H, Kim CK, Cho KF, Udeshi ND, 
            Carr SA, Ting AY
  TITLE     Engineered allostery in light-regulated LOV-Turbo enables precise 
            spatiotemporal control of proximity labeling in living cells.
  JOURNAL   Nat Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5.
  PUBMED    37188954
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5."
COMMENT     Sequence Label: V5-LOV-Turbo-NES
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2317..2358
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3739..3765
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     misc_feature    3785..4373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3838..3858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4256..4267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4393..4903
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4822..4840
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4913..5656
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5666..5722
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5723..6319
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6216..6236
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6418..6651
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6723..6844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6760..6779)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6814..6833
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6884..7019
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7019..7090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7091..7159,7160..7951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7309..7328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8122..8710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8611..8630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8864..8881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8998..9019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9034..9039,9040..9057,9058..9064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9072..9088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9077..9099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9096..9112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9096..9112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9131..9151
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9133..9151
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagcgcc accatgggca agcccatccc caaccccctg
     2341 ctgggcctgg acagcaccgg taccaaagac aacacagtgc ctctgaagct gatcgccctc
     2401 ctggccaatg gagagttcca cagcggcgag cagctgggcg aaaccctggg catgagcaga
     2461 gccgctatca acaagcacat ccagaccctt cgcgactggg gcgtcgacgt gttcagcgtg
     2521 cccggcaagg gctacagcct gcctgagcct atccccctgc tgaacgccaa gcagattcag
     2581 ggccagctgg aatttagagc caccaccctg gaaagaatcg agaagtcttt tgtgatcacc
     2641 gaccccagac tgcctgataa tcctatcatc ttcgtgtctg atagcttcct gcagctcacc
     2701 gagtattctc gggaagagat cctcggaaga aattgcagat tcctgcaggg cccagagaca
     2761 gatagagcca cagtgcggaa gatcagagat gccatcgaca accagacaga ggtgaccgtg
     2821 cagctgatca actacaccaa gtccggcaaa aagttctgga acgttttcca tctccaacct
     2881 atgagagact acaagggcga cgtgcagtac ttcatcggag ttcagctgga cggcaccgag
     2941 agactgcacg gcgccgcaga gcgggaagct gtgtgcctgg tcaagaagac cgccttccag
     3001 atcgccgtgg ctgtgctgcc cgtggtggac agcacaaacc agtacctgct ggacagaatc
     3061 ggcgagctga agtccggcga cgcttgtatc gccgaatacc agcaggccgg cagaggcagc
     3121 agaggccgga aatggttcag ccccttcggc gccaacctgt acctgagcat gttttggcgg
     3181 ctgaaaagag gccctgccgc cacaggcctg ggccccgtga taggaatcgt gatggccgag
     3241 gctctgagga agctgggtgt ggacaaagtg cgggtgaagt ggcctaacga cctgtacctg
     3301 caggacagaa agctggccgg catcctggtg gaactggctg gcatcaccgg cgacgccgct
     3361 cagattgtga tcggcgctgg catcaatgtg gccatgagac gcgtggaaga gagcgtggtc
     3421 aaccagggct ggatcacact gcaagaggcc ggtgtgaacc tggataggaa caccctggcc
     3481 gccaccctga tcagagagct gagagccgct ctggaattgt ttgagcagga gggcctggcc
     3541 ccttacctgc cacggtggga gaaactggat aacttcatca acaggcctgt gaagctgatc
     3601 atcggcgata aggaaatcct gggaatcagc cggggcatcg acaagcaggg agccctgctg
     3661 ctggaacaag atggagtgat taagccttgg atgggcggag aaatcagcct gcggtccgcc
     3721 gagaagggat ccctgcagct gcctcccctg gagcgcctga ccctggacta atagggccga
     3781 attcaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtctg
     4381 cagatatcat ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     4441 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     4501 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     4561 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     4621 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     4681 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     4741 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     4801 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     4861 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgtctag
     4921 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga
     4981 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5041 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     5101 tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     5161 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     5221 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     5281 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     5341 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     5401 cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga
     5461 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5521 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     5581 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     5641 tgacatgctc cccgggggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     5701 cgtggaggag aaccctggac ctaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5761 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5821 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6301 ccgcaagccc ggtgcctaac tgtttcgcct cggcgtccgg actagaggta cctttaagac
     6361 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6421 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6661 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6721 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6781 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6841 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6901 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6961 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
