LOCUS       sequence-392354-        9703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2954
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2457..2481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2489..2530
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2641..3229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2694..2714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3112..3123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3301..3534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3612..3733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3649..3668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3703..3722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3773..3908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3928..3947)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3929..3947)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3957..3974)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3957..3973)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3966..3988)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4115..4570
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4202..4221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4412..4433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4596..4700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4701..4769,4770..5561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4919..4938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5732..6320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6221..6240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6474..6491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6608..6629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6644..6649,6650..6667,6668..6674)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6682..6698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6687..6709
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6706..6722
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6706..6722
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6741..6761
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6743..6761
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6789..7015
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7016..7196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7243..7368
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7861..8094
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8279..8323
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8554..8764
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8576..8772
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8623..8758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8804..9406
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8804..8823)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9300..9320
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9449..9566
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcttcaccat gaataccaca gacaacggtg tcaactgtct
     1261 gtgtgctatc tgtggggaca gagcaacagg aaaacactat ggggcatcca gctgtgatgg
     1321 gtgcaagggt ttcttcagac gcagcattcg taagagtcac gtttattctt gcaggttcag
     1381 tcggcaatgt gttgttgaca aggacaaaag gaatcaatgt agatattgtc gattaagaaa
     1441 gtgttttaga gcgggaatga aaaaagaagc tgtacaaaat gaacgtgaca gaataagcac
     1501 cagaagaagc acatttgatg gcagcaacat cccctccatt aacacactgg cacaagctga
     1561 agttcggtct cgccagatct cagtctcaag ccctgggtca agcactgaca taaacgttaa
     1621 gaaaattgca agtattggtg atgtctgtga atctatgaaa cagcagctct tagtcttggt
     1681 ggaatgggct aaatatattc ctgccttctg tgaattacca ttggatgatc aggtggcact
     1741 gttgagagct cacgcagggg agcacttact gcttggagct acaaagagat ccatgatgta
     1801 taaagatatt ttgcttttgg gaaacaacta tgttattcac cgcaacagct gtgaagttga
     1861 gattagccgt gtggccaatc gtgttctaga tgagctggtt agaccatttc aagaaatcca
     1921 gattgatgac aatgagtatg cttgtttaaa ggcaattgta ttttttgatc cagatgcaaa
     1981 agggctaagc gatccagtaa aaattaagaa catgaggttc caagtgcaga tcggtttgga
     2041 ggactacatc aatgatcggc agtatgactc ccgggggagg tttggagagt tgcttctgct
     2101 cctgcccaca ctgcagagca tcacgtggca aatgattgag caaatacagt ttgttaaact
     2161 ttttgggatg gttaaaattg acaatctact tcaggaaatg ctattaggtg gggcttccaa
     2221 tgatggcagt catctccatc atccaatgca tccacatttg tctcaagacc cattaactgg
     2281 acaaactata cttttaggtc ccatgtcaac actggttcat gcagaccaga tctcaactcc
     2341 tgaaacccca ctcccttccc caccacaagg ctctgggcaa gaacagtaca aaatagctgc
     2401 aaaccaagca tcagtcattt cacaccagca tctctccaaa caaaagcaat tgtgagaccc
     2461 agctttcttg tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct
     2521 cgattctacg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     2581 tatatcttgt ggaaaggacg aaggaaactg aacgcaaatg attcgacgcg ttaagtcgac
     2641 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2701 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2761 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2821 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2881 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2941 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3001 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3061 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3121 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3181 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     3241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3301 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     3541 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3601 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3721 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     3781 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3841 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3901 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     3961 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4021 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4081 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4141 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4201 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4261 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4321 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4381 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4441 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4501 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4561 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4621 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4681 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4741 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4801 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4861 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4921 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4981 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5041 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5101 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5161 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5221 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5281 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5341 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5401 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5461 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5521 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5581 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5641 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5941 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6001 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6061 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6121 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6181 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6241 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6301 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6361 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6421 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6481 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6541 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6601 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6661 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6721 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6781 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     6841 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     6901 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     6961 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7081 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7141 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7201 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7261 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7321 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7381 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7441 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7501 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7561 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7621 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7681 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7741 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7801 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7861 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7921 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     7981 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8041 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8101 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8161 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8221 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8281 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8341 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8401 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8461 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     8521 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     8581 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8701 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8761 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     8821 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     8881 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     8941 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9001 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9061 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9121 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9181 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     9241 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     9301 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     9361 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
     9421 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9481 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     9541 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     9601 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
     9661 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
