LOCUS       sequence-392356-       10396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3484
FEATURES             Location/Qualifiers
     source          1..10396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3150..3174)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3182..3223
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3334..3922
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3387..3407)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3805..3816)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3994..4227
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4305..4426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4342..4361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4396..4415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4466..4601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4621..4640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4622..4640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4650..4667)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4650..4666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4659..4681)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4808..5263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4895..4914)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5105..5126
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5289..5393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5394..5462,5463..6254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5612..5631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6425..7013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6914..6933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7167..7184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7301..7322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7337..7342,7343..7360,7361..7367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7375..7391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7380..7402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7399..7415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7399..7415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7434..7454
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7436..7454
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7482..7708
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7709..7889
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7936..8061
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8554..8787
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8972..9016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9247..9457
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9269..9465
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9316..9451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9497..10099
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9497..9516)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9993..10013
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10142..10259
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggctga ggcggccccg gccccgacat ctgaatggga
     1261 ctccgagtgc cttacatccc tgcagcccct tcctcttcct acacccccag cagcaaatga
     1321 ggcacacctg cagacagcag ctatctctct gtggacagtg gtggccgccg tgcaggctat
     1381 agagaggaag gtggagatcc acagccggcg actcctacac ctagaaggtc ggacagggac
     1441 agcagagaag aaactagcca gctgtgaaaa gacagttacc gagcttggga accagctgga
     1501 gggcaagtgg gccgtgctgg gaaccctgct gcaggagtac gggctgctgc agaggcggct
     1561 ggagaacttg gagaacctgc tgcgcaacag gaacttctgg atcctgcggc tccctccagg
     1621 tattaaggga gatatcccaa aggtgcctgt ggcatttgat gatgtctcca tctacttttc
     1681 cactccagag tgggaaaaat tagaagaatg gcaaaaggaa ctttacaaga atatcatgaa
     1741 gggcaactac gagtctctca tctccatgga ttatgctata aatcaacctg atgtcttatc
     1801 tcagattcaa ccagaagggg aacataatac agaggaccag gcagggccag aggaaagtga
     1861 gattcccaca gaccccagtg aagagcctgg tatttcaaca tcagatattc tgtcttggat
     1921 taaacaagaa gaagagcctc aggttggggc cccaccggag tccaaggaga gtgacgtgta
     1981 caaaagcact tatgctgatg aagagcttgt catcaaagct gaaggccttg ctagatcctc
     2041 gttgtgccct gaggttccag tccctttctc ttctccacca gcagcagcaa aggatgcttt
     2101 ttcagatgtg gctttcaaaa gccagcagtc tacatccatg acaccttttg gacgtccagc
     2161 cactgacctg cctgaagcct ctgagggaca agtgactttt actcagttgg gtagctatcc
     2221 cctcccacct ccagttggcg agcaggtgtt ctcatgccac cactgtggca agaatctcag
     2281 ccaagacatg ttgctgaccc accaatgtag ccatgctact gagcacccct taccctgtgc
     2341 ccagtgccct aagcacttta ctccacaggc ggacctcagc agcacctccc aggaccatgc
     2401 cagcgagaca ccccccacct gcccacactg tgccaggact tttactcacc catcaagact
     2461 tacctaccat cttcgggtcc ataacagcac tgagcgtcct ttcccctgtc ctgattgccc
     2521 caagcgcttt gctgaccagg ctcgactcac cagccaccgg agagctcatg caagcgaaag
     2581 gcccttccgc tgtgcccagt gcggcaggag cttcagcttg aaaatcagcc tcctgctcca
     2641 ccagcggggt catgcacaag agcgcccttt ctcctgccct cagtgtggca ttgacttcaa
     2701 cggccactcg gccctgatcc gccaccagat gatccacaca ggcgagcgtc cttacccctg
     2761 cactgactgc agtaagagct tcatgcgcaa ggagcacctg ctgaaccacc ggcggctgca
     2821 cacaggcgag cggcccttca gttgtcctca ctgtggcaag agcttcatcc gcaagcacca
     2881 cctaatgaaa caccagcgca tccacaccgg ggagcggccc tacccctgct cctactgtgg
     2941 caggagcttc cgctacaaac agacactcaa ggaccacctc cgttcaggcc acaatggagg
     3001 ctgtgggggt gatagtgacc catcaggtca gccacccaac ccaccaggtc ccctcataac
     3061 tgggcttgaa acttctggcc tgggtgtcaa cactgaaggt ctagagacca accagtggta
     3121 tggggaaggg agtggagggg gagttttgta cccaactttc ttgtacaaag tggttgatat
     3181 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc
     3241 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcacta
     3301 tttttatccg agatcagtac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     3361 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3421 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3481 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3541 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3601 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3661 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3721 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3781 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3841 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3901 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3961 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4021 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4081 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4141 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4201 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     4261 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4441 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4501 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4561 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4621 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4681 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4741 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4801 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4861 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4921 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4981 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5041 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5101 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5161 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5221 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7201 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7261 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7321 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7381 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7441 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7501 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7561 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7621 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7681 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7741 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7801 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7861 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7921 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7981 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     8041 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     8101 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     8161 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     8221 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     8281 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     8341 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8401 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8461 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8521 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8581 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8641 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8701 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8761 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8821 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8881 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8941 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9001 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9061 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9121 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9181 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     9241 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     9301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     9481 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9541 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9601 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9661 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9721 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9781 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9841 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9901 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9961 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
    10021 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
    10081 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
    10141 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    10201 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    10261 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
    10321 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
    10381 gaaaggagtg ggaatt
//
