LOCUS       sequence-392365-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 backbone expressing sgRNA to edit the C-terminus of human 
            PLIN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, 
            Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, 
            Kampmann M, Kopito RR, Faghri F, Olzmann JA
  TITLE     Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid 
            droplet regulation.
  JOURNAL   Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001.
  PUBMED    37494933
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001."
COMMENT     Sequence Label: pX330 PLIN2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        75..150
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        247..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cggttatttc ggatttcgtg gctttatata tcttgtggaa aggacgaaac accgacagca
       61 tgcactagtg ataggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      121 caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga aatagcaagt
      181 taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa tggctctaga
      241 ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca tgaccttatg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      541 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      601 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggggggagac
      661 acacgggatt gcaaagagaa agaaaaaagg aagagaaaaa aaaagaaagg aaaaaaagaa
      721 ggcaaacggt tagaggagag gttgaagagg ggagagaaga agtagaagag tagtagaggt
      781 tggggggaag agttggagaa ggaaaagagc tttggcaggt agaatagccg aatgagagac
      841 cgcacaatag gagagcccgg ggaagagagg aagatggaga acgagagaac gaagtggatg
      901 atggggaaac agggaaggga ggagtgtatg gaggggtgta ctggcagaga taaaggcggg
      961 taatatgggt gggcggagag cgagcgtgta tgaggatagg
//
