LOCUS       sequence-392370-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous 
            human PLIN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, 
            Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, 
            Kampmann M, Kopito RR, Faghri F, Olzmann JA
  TITLE     Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid 
            droplet regulation.
  JOURNAL   Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001.
  PUBMED    37494933
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001."
COMMENT     Sequence Label: pUC19/PLIN2 GFP-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgacgttga atgattacgc cagcttgcat gcctgcaggt cgactctaga gaggattttt
       61 aaaagccaaa gaatccacat atgtggtgga cacataacca gcctactgtc atcgaactaa
      121 tttatacata gcactgttgg atccactgct aattttaagt tttattttta gcacattgag
      181 tcacgtactc ttgcaattgc ccgcaacctg actcagcagc tccagaccac gtgccacacc
      241 ctcctgtcca acatccaagg tgtaccacag aacatccaag atcaagccaa gcacatgggg
      301 gtgatggcag gcgacatcta ctcagtgttc cgcaatgctg cctcctttaa agaagtgtct
      361 gacagcctcc tcacttctag caaggggcag ctgcagaaaa tgaaggaatc tttagatgac
      421 gtgatggatt atcttgttaa caacacgccc ctcaactggc tggtaggtcc cttttatcct
      481 cagctgactg agtctcagaa tgctcaggac caaggtgcag agatggacaa gagcagccag
      541 gagacccagc gatctgagca taaaactcat ggcagcggca tggtcagcaa gggggaagag
      601 ctgttcactg gtgtagtccc catacttgta gagcttgacg gtgacgtgaa cggacataaa
      661 tttagcgttt caggcgaggg agagggtgac gcgacatatg ggaaactgac actcaagttc
      721 atatgtacta ctggtaagct ccctgtgccc tggccgactc tggttaccac gcttacttat
      781 ggtgtacaat gtttcagtag atatccggac catatgaagc aacatgactt ctttaaaagt
      841 gccatgcctg aagggtatgt acaagaacgc acaatttttt ttaaagacga tggtaattat
      901 aagactaggg cggaggttaa attcgaggga gatacgctgg taaaccgcat tgagcttaag
      961 ggcattgatt tcaaagagga cggcacattt tgggacacaa
//
