LOCUS       sequence-392371-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous 
            human PLIN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, 
            Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, 
            Kampmann M, Kopito RR, Faghri F, Olzmann JA
  TITLE     Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid 
            droplet regulation.
  JOURNAL   Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001.
  PUBMED    37494933
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 Jul 18:S1534-5807(23)00331-3. doi: 
            10.1016/j.devcel.2023.07.001."
COMMENT     Sequence Label: pUC19/PLIN2 GFP-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccacgcaaga tacatccctt caggatggct gcttatttat aatgtgaaaa ttcgcggggt
       61 taactttaca agcaacgggc cagtgatgca gaagaagaca ttgggctggg aagcctttac
      121 cgaaacactg taccctgcag acggcggcct ggaaggaagg aacgatatgg cccttaagtt
      181 ggtgggcggt agccacctta tcgctaatat caaaacaacc taccgcagta aaaagccagc
      241 taaaaatctt aagatgccag gggtgtatta tgttgattat agactggaac gcataaaaga
      301 ggccaataat gaaacatacg tcgaacagca tgaggtagcc gtagcccggt actgtgatct
      361 ccccagcaag ttggggcata aacttaacta gtaaacctgg ccgtatcact agtgcatgct
      421 gtggccagac agatgacacc ttttgttatg ttgaaattaa cttgctaggc aaccctaaat
      481 tgggaagcaa gtagctagta taaaggccct caattgtagt tgtttccagc tgaattaaga
      541 gctttaaagt ttctggcatt agcagatgat ttctgttcac ctggtaagaa aagaatgata
      601 ggcttgtcag agcctatagc cagaactcag aaaaaattca aatgcactta tgttctcatt
      661 ctatggccat tgtgttgcct ctgttactgt ttgtattgaa taaaaacatc ttcatgtggg
      721 ctggggtaga aactggtgtc tgctctggtg tgatctgaaa ggcgtcttca ctgctttatc
      781 tcatgatgct tgcttgtaaa acttgatttt agtttttcat ttctcaaata ggaatactac
      841 ctttgaattc aataaaattc actgcaggat agaccagtta catgctgttt gttccatatg
      901 ctttgtgtgt tgctttcgta gagctgctta acctgcatga cagagttatt atacatacat
      961 gaatttcaat tggcctgtgg gaggtccccg accagaaggg
//
