LOCUS       sequence-39238-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pleiades (Ple) MiniPromoter expression pattern described in 
            Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et
            al., MTM, 2014 (PMID: 24761428)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1602
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..131
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     regulatory      142..151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          201..333
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
       61 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      121 gttgtaaaac gacgggatcc gccatggtgg cggaagttcc tatacctttt gaagaatagg
      181 aacttcggaa taggaacttc ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      241 aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt tctattggtc
      301 tccttaaacc tgtcttgtaa ccttgatact tacggcgcgc cacgcgtggc cggcctttct
      361 aaacagtaag ttgagtataa tccgtagggg aaggccaggt ataatccgta ggggaaggcc
      421 agaagcaaat ttacaactct tccactgcaa aagaggtgcg agttactctg atagcaaagg
      481 agctaagaag ggccccgtgc ccaattcacc tatccccggc cacagaagcg cacaccgcag
      541 ctagctagcg gggaccccag gacgatccga ccgcagcccc cggcgcccca aagtccggca
      601 gctggggtgt tgtcgccagc gcctgcgcct cctgggagct tccaccgaga cgccctcgcc
      661 agagcccagg aggagtcggg cccagg
//
