LOCUS       sequence-392479-       15733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of rodent iPS cells from somatic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15733)
  AUTHORS   Yamaguchi T, Hamanaka S, Nakauchi H
  TITLE     The generation and maintenance of rat induced pluripotent stem 
            cells.
  JOURNAL   Methods Mol Biol. 2014;1210:143-50. doi: 
            10.1007/978-1-4939-1435-7_11.
  PUBMED    25173166
REFERENCE   2  (bases 1 to 15733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2014"; volume: "1210:143-50. doi"; pages: " 
            10.1007/978-1-4939-1435-7_11"
COMMENT     Sequence Label: Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)
FEATURES             Location/Qualifiers
     source          1..15733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(10..12,13..15,16..729)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10..12,13..15,16..726)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(316..335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    753..1341
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(1224..1235)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1860..2040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2066..2083)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2072..2296
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2342..2770
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2429..2448)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2639..2660
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2779..2799)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2784..3113
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2964..3099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3026..3045
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3161..3208
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3227..3601
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3731..3852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3768..3787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3822..3841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3901..3917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3901..3917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3914..3936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3925..3941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3949..3955,3956..3973,3974..3979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3994..4015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4132..4149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4303..4891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4383..4402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5062..5853,5854..5922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5685..5704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5923..6027)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6102..6121)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6293..6672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6674..6872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6890..7070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7117..7242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7735..7968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8153..8197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8471..8588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8630..8944
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8638..8656
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8674..8692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8709..8727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8745..8763
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8781..8799
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8816..8834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8852..8870
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8917..8941
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10038..10103
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             11538..11591
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    12575..13163
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(13046..13057)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        13173..14384
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     14221..14240
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(14397..15014,15015..15020,15021..15056,15057..15059,
                     15060..15095,15096..15098,15099..15134,15135..15143)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(15155..15730,15731..15733,1..9)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(15323..15340)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     15550..15569
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      181 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      481 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      541 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      601 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      721 tacaagtaac tcgagggatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
      781 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
      841 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
      901 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
      961 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     1021 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     1081 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     1141 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     1201 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     1261 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     1321 ccctttgggc cgcctccccg catcgatacc gtcgagacct agaaaaacat ggagcaatca
     1381 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     1441 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     1501 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     1561 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     1621 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     1681 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     1741 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     1801 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     1861 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1921 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1981 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2041 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2101 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2161 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2221 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2281 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2341 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2401 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2461 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2521 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2581 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2641 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2701 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2761 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     2881 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2941 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3001 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     3061 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     3121 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     3181 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     3241 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     3301 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     3361 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     3421 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     3481 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     3541 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     3601 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     3661 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     3721 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3841 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     3901 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3961 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4141 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4201 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4261 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4321 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4381 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4441 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4501 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4561 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4621 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4681 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4741 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4801 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4861 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4921 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4981 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5041 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5101 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5161 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5221 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5281 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5341 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5401 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5461 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5521 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5581 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5641 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5701 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5761 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5821 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5881 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5941 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6001 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     6061 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     6121 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     6181 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     6241 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     6301 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6361 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6421 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6481 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6541 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6601 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6661 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     6721 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6781 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     6841 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
     6901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7081 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7141 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7201 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7261 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7321 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7381 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7441 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7501 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7561 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7621 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7681 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7741 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7801 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7861 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     7921 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7981 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8041 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8101 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8161 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8221 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8281 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8341 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8401 tggagagaga gacagagaca gatccattcg attagtgaac ggatctacaa atggcagtat
     8461 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     8521 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     8581 aaaattttcg ggtttattac agggacagca gaaattcact ttgaattgcg agtttactcc
     8641 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc
     8701 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     8761 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct
     8821 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     8881 aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat
     8941 cgcctggaga attgcgtacg gccagcatgg ctggacacct ggcttcagac ttcgccttct
     9001 cacccccacc aggtgggggt gatgggtcag cagggctgga gccgggctgg gtggattctc
     9061 gaacctggct aagcttccaa gggcctccag gtgggcctgg aatcggacca ggctcagagg
     9121 tattggggat ctccccatgt ccgcccgcat acgagttctg cggagggatg gcatactgtg
     9181 gacctcaggt tggactgggc ctagtccccc aagttggcgt ggagactttg cagcctgagg
     9241 gccaggcagg agcacgagtg gaaagcaact cagagggaac ctcctctgag ccctgtgccg
     9301 accgccccaa tgccgtgaag ttggagaagg tggaaccaac tcccgaggag tcccaggaca
     9361 tgaaagccct gcagaaggag ctagaacagt ttgccaagct gctgaagcag aagaggatca
     9421 ccttggggta cacccaggcc gacgtggggc tcaccctggg cgttctcttt ggaaaggtgt
     9481 tcagccagac caccatctgt cgcttcgagg ccttgcagct cagccttaag aacatgtgta
     9541 agctgcggcc cctgctggag aagtgggtgg aggaagccga caacaatgag aaccttcagg
     9601 agatatgcaa atcggagacc ctggtgcagg cccggaagag aaagcgaact agcattgaga
     9661 accgtgtgag gtggagtctg gagaccatgt ttctgaagtg cccgaagccc tccctacagc
     9721 agatcactca catcgccaat cagcttgggc tagagaagga tgtggttcga gtatggttct
     9781 gtaaccggcg ccagaagggc aaaagatcaa gtattgagta ttcccaacga gaagagtatg
     9841 aggctacagg gacacctttc ccaggggggg ctgtatcctt tcctctgccc ccaggtcccc
     9901 actttggcac cccaggctat ggaagccccc acttcaccac actctactca gtcccttttc
     9961 ctgagggcga ggcctttccc tctgttcccg tcactgctct gggctctccc atgcattcaa
    10021 acatcgatgg ttctggagtg aaacagactt tgaattttga ccttctcaag ttggcgggag
    10081 acgtggagtc caacccaggg ccctctagaa tggctgtcag cgacgctctg ctcccgtcct
    10141 tctccacgtt cgcgtccggc ccggcgggaa gggagaagac actgcgtcca gcaggtgccc
    10201 cgactaaccg ttggcgtgag gaactctctc acatgaagcg acttccccca cttcccggcc
    10261 gcccctacga cctggcggcg acggtggcca cagacctgga gagtggcgga gctggtgcag
    10321 cttgcagcag taacaacccg gccctcctag cccggaggga gaccgaggag ttcaacgacc
    10381 tcctggacct agactttatc ctttccaact cgctaaccca ccaggaatcg gtggccgcca
    10441 ccgtgaccac ctcggcgtca gcttcatcct cgtcttcccc agcgagcagc ggccctgcca
    10501 gcgcgccctc cacctgcagc ttcagctatc cgatccgggc cgggggtgac ccgggcgtgg
    10561 ctgccagcaa cacaggtgga gggctcctct acagccgaga atctgcgcca cctcccacgg
    10621 cccccttcaa cctggcggac atcaatgacg tgagcccctc gggcggcttc gtggctgagc
    10681 tcctgcggcc ggagttggac ccagtataca ttccgccaca gcagcctcag ccgccaggtg
    10741 gcgggctgat gggcaagttt gtgctgaagg cgtctctgac cacccctggc agcgagtaca
    10801 gcagcccttc ggtcatcagt gttagcaaag gaagcccaga cggcagccac cccgtggtag
    10861 tggcgcccta cagcggtggc ccgccgcgca tgtgccccaa gattaagcaa gaggcggtcc
    10921 cgtcctgcac ggtcagccgg tccctagagg cccatttgag cgctggaccc cagctcagca
    10981 acggccaccg gcccaacaca cacgacttcc ccctggggcg gcagctcccc accaggacta
    11041 cccctacact gagtcccgag gaactgctga acagcaggga ctgtcaccct ggcctgcctc
    11101 ttcccccagg attccatccc catccggggc ccaactaccc tcctttcctg ccagaccaga
    11161 tgcagtcaca agtcccctct ctccattatc aagagctcat gccaccgggt tcctgcctgc
    11221 cagaggagcc caagccaaag aggggaagaa ggtcgtggcc ccggaaaaga acagccaccc
    11281 acacttgtga ctatgcaggc tgtggcaaaa cctataccaa gagttctcat ctcaaggcac
    11341 acctgcgaac tcacacaggc gagaaacctt accactgtga ctgggacggc tgtgggtgga
    11401 aattcgcccg ctccgatgaa ctgaccaggc actaccgcaa acacacaggg caccggccct
    11461 ttcagtgcca gaagtgcgac agggcctttt ccaggtcgga ccaccttgcc ttacacatga
    11521 agaggcactt ttccggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga
    11581 atcctggccc agttaacatg tataacatga tggagacgga gctgaagccg ccgggcccgc
    11641 agcaagcttc ggggggcggc ggcggaggag gcaacgccac ggcggcggcg accggcggca
    11701 accagaagaa cagcccggac cgcgtcaaga ggcccatgaa cgccttcatg gtatggtccc
    11761 gggggcagcg gcgtaagatg gcccaggaga accccaagat gcacaactcg gagatcagca
    11821 agcgcctggg cgcggagtgg aaacttttgt ccgagaccga gaagcggccg ttcatcgacg
    11881 aggccaagcg gctgcgcgct ctgcacatga aggagcaccc ggattataaa taccggccgc
    11941 ggcggaaaac caagacgctc atgaagaagg ataagtacac gcttcccgga ggcttgctgg
    12001 cccccggcgg gaacagcatg gcgagcgggg ttggggtggg cgccggcctg ggtgcgggcg
    12061 tgaaccagcg catggacagc tacgcgcaca tgaacggctg gagcaacggc agctacagca
    12121 tgatgcagga gcagctgggc tacccgcagc acccgggcct caacgctcac ggcgcggcac
    12181 agatgcaacc gatgcaccgc tacgacgtca gcgccctgca gtacaactcc atgaccagct
    12241 cgcagaccta catgaacggc tcgcccacct acagcatgtc ctactcgcag cagggcaccc
    12301 ccggtatggc gctgggctcc atgggctctg tggtcaagtc cgaggccagc tccagccccc
    12361 ccgtggttac ctcttcctcc cactccaggg cgccctgcca ggccggggac ctccgggaca
    12421 tgatcagcat gtacctcccc ggcgccgagg tgccggagcc cgctgcgccc agtagactgc
    12481 acatggccca gcactaccag agcggcccgg tgcccggcac ggccattaac ggcacactgc
    12541 ccctgtcgca catgtgaacc ggtctcgaga attcaatcaa cctctggatt acaaaatttg
    12601 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    12661 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    12721 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    12781 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    12841 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    12901 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    12961 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    13021 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    13081 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    13141 ctccctttgg gccgcctccc cgctagcgat ctggcctccg cgccgggttt tggcgcctcc
    13201 cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg
    13261 tcctgatcct tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt
    13321 agaaccccag tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact
    13381 ggttttcttt ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc
    13441 ggagggatct ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc
    13501 acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga
    13561 tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg
    13621 ctcggtggga cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca
    13681 aggttgccct gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct
    13741 tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg
    13801 tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg cgggaaagct cttattcggg
    13861 tgagatgggc tggggcacca tctggggacc ctgacgtgaa gtttgtcact gactggagaa
    13921 ctcggtttgt cgtctgttgc gggggcggca gttatggcgg tgccgttggg cagtgcaccc
    13981 gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata
    14041 atgcagggtg gggccacctg ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc
    14101 agggttcggg cctagggtag gctctcctga atcgacaggc gccggacctc tggtgagggg
    14161 agggataagt gaggcgtcag tttctttggt cggttttatg tacctatctt cttaagtagc
    14221 tgaagctccg gttttgaact atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt
    14281 aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa ttttcagtgt tagactagta
    14341 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacaagctt gtcaccatgt
    14401 ctagactgga caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta
    14461 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
    14521 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
    14581 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
    14641 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
    14701 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
    14761 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
    14821 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
    14881 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
    14941 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
    15001 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
    15061 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
    15121 accttgacat gctccccggg taactcgaga tccgcccctc tccctccccc cccccntaac
    15181 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
    15241 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
    15301 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
    15361 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
    15421 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
    15481 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
    15541 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
    15601 ccccattgta tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg
    15661 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
    15721 cgatgataat atg
//
