LOCUS       sequence-392525-       10927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of Expandable megakaryocyte cell lines from human iPS 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita K, 
            Koike T, Harimoto K, Dohda T, Watanabe A, Okita K, Takahashi N, 
            Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K
  TITLE     Expandable megakaryocyte cell lines enable clinically applicable 
            generation of platelets from human induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2014 Apr 3;14(4):535-48. doi: 
            10.1016/j.stem.2014.01.011. Epub 2014 Feb 13.
  PUBMED    24529595
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2014.01.011"; journalName: "Cell Stem Cell"; date: 
            "2014-04-3- 3"; volume: "14"; issue: "4"; pages: "535-48"
COMMENT     Sequence Label: CS-TRE-BclXL-Ubc-rtTA
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..94)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        266..645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        647..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             863..1043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1090..1215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1708..1941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2126..2170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2603..2917
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2611..2629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2647..2665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2682..2700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2718..2736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2754..2772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2789..2807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2825..2843
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2890..2914
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3659..4870
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4707..4726
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(4883..5500,5501..5506,5507..5542,5543..5545,5546..5581,
                     5582..5584,5585..5620,5621..5629)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5653..6241
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5706..5726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6124..6135)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6760..6940
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6966..6983)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6972..7196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7242..7670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7329..7348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7539..7560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7679..7699)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7684..8013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7864..7999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7926..7945
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8061..8108
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8127..8501
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8631..8752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8668..8687)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8722..8741
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8801..8817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8801..8817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8814..8836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8825..8841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8849..8855,8856..8873,8874..8879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8894..8915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9032..9049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9203..9791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9283..9302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9962..10753,10754..10822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10585..10604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10823..10927)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      841 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1021 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1081 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1141 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1201 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1261 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1321 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1381 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1441 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1501 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1561 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1621 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1681 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1741 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1801 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1861 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1921 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1981 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2041 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2101 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2161 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2221 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2281 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2341 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2401 tcgattagtg aacggatcta caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagaaattc actttgaatt gcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2641 gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca gtgatagaga
     2701 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2761 cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg tatgtcgagt
     2821 ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggaggccta
     2881 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agaattgcgt acggccagca
     2941 tgtctcagag caaccgggag ctggtggttg actttctctc ctacaagctt tcccagaaag
     3001 gatacagctg gagtcagttt agtgatgtgg aagagaacag gactgaggcc ccagaaggga
     3061 ctgaatcgga gatggagacc cccagtgcca tcaatggcaa cccatcctgg cacctggcag
     3121 acagccccgc ggtgaatgga gccactggcc acagcagcag tttggatgcc cgggaggtga
     3181 tccccatggc agcagtaaag caagcgctga gggaggcagg cgacgagttt gaactgcggt
     3241 accggcgggc attcagtgac ctgacatccc agctccacat caccccaggg acagcatatc
     3301 agagctttga acaggtagtg aatgaactct tccgggatgg ggtaaactgg ggtcgcattg
     3361 tggccttttt ctccttcggc ggggcactgt gcgtggaaag cgtagacaag gagatgcagg
     3421 tattggtgag tcggatcgca gcttggatgg ccacttacct gaatgaccac ctagagcctt
     3481 ggatccagga gaacggcggc tgggatactt ttgtggaact ctatgggaac aatgcagcag
     3541 ccgagagccg aaagggccag gaacgcttca accgctggtt cctgacgggc atgactgtgg
     3601 ccggcgtggt tctgctgggc tcactcttca gtcggaaatg agaattcgct agcgatctgg
     3661 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     3721 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     3781 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     3841 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     3901 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     3961 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     4021 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     4081 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     4141 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     4201 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     4261 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     4321 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     4381 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     4441 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     4501 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     4561 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     4621 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     4681 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     4741 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     4801 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     4861 tttgttagac aagcttgtca ccatgtctag actggacaag agcaaagtca taaacggcgc
     4921 tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     4981 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5041 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     5101 gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc tcctctcaca
     5161 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5221 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5281 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5341 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     5401 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5461 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccggccg acgcccttga
     5521 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     5581 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac tcgagggatc
     5641 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5701 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5761 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5821 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5881 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5941 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6001 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     6061 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6121 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6181 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6241 catcgatacc gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     6301 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6361 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6421 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     6481 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     6541 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     6601 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     6661 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     6721 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     6781 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6841 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6901 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     6961 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7021 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7081 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7141 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7201 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     7261 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7321 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7381 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7441 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7501 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7561 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7621 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     7681 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7741 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7801 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7861 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7921 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     7981 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8041 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     8101 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     8161 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     8221 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     8281 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     8341 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     8401 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     8461 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     8521 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     8581 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     8641 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8701 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8761 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8821 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8881 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8941 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9001 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9061 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9121 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9181 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9241 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9301 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9361 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9421 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9481 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9541 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9601 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9661 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9721 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9781 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9841 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9901 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9961 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10021 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10081 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10141 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10201 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10261 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10321 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10381 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10441 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10501 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10561 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10621 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10681 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10741 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10801 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10861 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10921 ttccgcg
//
