LOCUS       sequence-392541-        9407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 9407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: 1K245-->R GLUT1-GFP
FEATURES             Location/Qualifiers
     source          1..9407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3742..3766)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3777..3779,3780..3782,3783..4496)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3810..3838)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4433..4453
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    join(4534..5108,5109..5111,5112..5120)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4701..4718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4928..4947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5140..5739
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5140..5159)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5636..5656
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6113..6538
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6641..6663)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6799..6819)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6804..7133
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6984..7119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7155..7174
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7255..7272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7426..8014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7506..7525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8185..8976,8977..9045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8808..8827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9046..9150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9218..9236
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9336..9407,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca tggagcccag cagcaagaag ctgacgggtc
     2281 gcctcatgct ggccgtggga ggagcagtgc ttggctccct gcagtttggc tacaacactg
     2341 gagtcatcaa tgccccccag aaggtgatcg aggagttcta caaccagaca tgggtccacc
     2401 gctatgggga gagcatcctg cccaccacgc tcaccacgct ctggtccctc tcagtggcca
     2461 tcttttctgt tgggggcatg attggctcct tctctgtggg ccttttcgtt aaccgctttg
     2521 gccggcggaa ttcaatgctg atgatgaacc tgctggcctt cgtgtccgcc gtgctcatgg
     2581 gcttctcgaa actgggcaag tcctttgaga tgctgatcct gggccgcttc atcatcggtg
     2641 tgtactgcgg cctgaccaca ggcttcgtgc ccatgtatgt gggtgaagtg tcacccacag
     2701 cccttcgtgg ggccctgggc accctgcacc agctgggcat cgtcgtcggc atcctcatcg
     2761 cccaggtgtt cggcctggac tccatcatgg gcaacaagga cctgtggccc ctgctgctga
     2821 gcatcatctt catcccggcc ctgctgcagt gcatcgtgct gcccttctgc cccgagagtc
     2881 cccgcttcct gctcatcaac cgcaacgagg agaaccgggc caagagtgtg ctaaagaagc
     2941 tgcgcgggac agctgacgtg acccatgacc tgcaggagat gcgggaagag agtcggcaga
     3001 tgatgcggga gaagaaggtc accatcctgg agctgttccg ctcccccgcc taccgccagc
     3061 ccatcctcat cgctgtggtg ctgcagctgt cccagcagct gtctggcatc aacgctgtct
     3121 tctattactc cacgagcatc ttcgagaagg cgggggtgca gcagcctgtg tatgccacca
     3181 ttggctccgg tatcgtcaac acggccttca ctgtcgtgtc gctgtttgtg gtggagcgag
     3241 caggccggcg gaccctgcac ctcataggcc tcgctggcat ggcgggttgt gccatactca
     3301 tgaccatcgc gctagcactg ctggagcagc taccctggat gtcctatctg agcatcgtgg
     3361 ccatctttgg ctttgtggcc ttctttgaag tgggtcctgg ccccatccca tggttcatcg
     3421 tggctgaact cttcagccag ggtccacgtc cagctgccat tgccgttgca ggcttctcca
     3481 actggacctc aaatttcatt gtgggcatgt gcttccagta tgtggagcaa ctgtgtggtc
     3541 cctacgtctt catcatcttc actgtgctcc tggttctgtt cttcatcttc acctacttca
     3601 aagttcctga gactaaaggc cggaccttcg atgagatcgc ttccggcttc cggcaggggg
     3661 gagccagcca aagtgacaag acacccgagg agctgttcca tcccctgggg gctgattccc
     3721 aagtgaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggttcga tctagaatgg
     3781 ctagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     3841 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa
     3901 agcttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     3961 tcactacttt ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc
     4021 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
     4081 aagatgacgg gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
     4141 atcgtatcga gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac
     4201 tcgagtacaa ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
     4261 tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc
     4321 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     4381 tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     4441 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaataattaa
     4501 ttaacggatc cggaattccc cccccccccc ccccccctct ccctcccccc cccctaacgt
     4561 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4621 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4681 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4741 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4801 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4861 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4921 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4981 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5041 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     5101 atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg
     5161 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     5221 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     5281 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5341 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5401 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5461 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5521 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5581 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5641 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5701 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     5761 cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc
     5821 cgccgacccc gcacccgccc ccgaggccca ccgactctag tcgagggctg cagcgctgca
     5881 gaggccgagt gcagaactgc tccaaaggga cctcaaggct ttccgaggga cactaggctg
     5941 actccatcga gccagtgtag agataagctt atcgattagt ccaatttgtt aaagacagga
     6001 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     6061 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6121 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     6181 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
     6241 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     6301 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     6361 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     6421 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     6481 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     6541 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     6601 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     6661 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6721 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     6781 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6841 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6901 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6961 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7021 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7081 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     7141 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7861 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     8521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     8821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9241 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat
     9301 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
     9361 atagtaatca attacggggt cattagttca tagcccatat atggagt
//
