LOCUS       sequence-392586-        6088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AAV2 Rep and AAV.cc84 Cap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Gonzalez TJ, Mitchell-Dick A, Blondel LO, Fanous MM, Hull JA, Oh DK,
            Moller-Tank S, Castellanos Rivera RM, Piedrahita JA, Asokan A
  TITLE     Structure-guided AAV capsid evolution strategies for enhanced CNS 
            gene delivery.
  JOURNAL   Nat Protoc. 2023 Sep 21. doi: 10.1038/s41596-023-00875-y.
  PUBMED    37735235
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2023 Sep 21. doi: 10.1038/s41596-023-00875-y."
COMMENT     Sequence Label: pAAV.cc84-mut
FEATURES             Location/Qualifiers
     source          1..6088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4077..4088
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     rep_origin      complement(4339..4927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4419..4438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5098..5889,5890..5958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5721..5740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5959..6063)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatatcctta aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
       61 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
      121 gaggtttgaa cgcgcagcca ccacgccggg gttttacgag attgtgatta aggtccccag
      181 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
      241 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
      301 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
      361 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
      421 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
      481 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
      541 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
      601 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
      661 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
      721 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
      781 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
      841 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
      901 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
      961 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
     1021 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
     1081 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
     1141 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
     1201 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
     1261 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
     1321 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
     1381 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
     1441 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
     1501 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
     1561 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
     1621 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
     1681 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
     1741 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
     1801 gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
     1861 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
     1921 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga
     1981 tttggatgac tgcatctttg aacaataaat gatttaaatc aggtatggct gccgatggtt
     2041 atcttccaga ttggctcgag gacaacctta gtgaaggaat tcgcgagtgg tgggctttga
     2101 aacctggagc ccctcaaccc aaggcaaatc aacaacatca agacaacgct cgaggtcttg
     2161 tgcttccggg ttacaaatac cttggacccg gcaacggact cgacaagggg gagccggtca
     2221 acgcagcaga cgcggcggcc ctcgagcacg acaaggccta cgaccagcag ctcaaggccg
     2281 gagacaaccc gtacctcaag tacaaccacg ccgacgccga gttccaggag cggctcaaag
     2341 aagatacgtc ttttgggggc aacctcgggc gagcagtctt ccaggccaaa aagaggcttc
     2401 ttgaacctct tggtctggtt gaggaagcgg ctaagacggc tcctggaaag aagaggcctg
     2461 tagagcagtc tcctcaggaa ccggactcct ccgcgggtat tggcaaatcg ggtgcacagc
     2521 ccgctaaaaa gagactcaat ttcggtcaga ctggcgacac agagtcagtc ccagaccctc
     2581 aaccaatcgg agaacctccc gcagccccct caggtgtggg atctcttaca atggcttcag
     2641 gtggtggcgc accagtggca gacaataacg aaggtgccga tggagtgggt agttcctcgg
     2701 gaaattggca ttgcgattcc caatggctgg gggacagagt catcaccacc agcacccgaa
     2761 cctgggccct gcccacctac aacaatcacc tctacaagca aatctccaac agcacatctg
     2821 gaggatcttc aaatgacaac gcctacttcg gctacagcac cccctggggg tattttgact
     2881 tcaacagatt ccactgccac ttctcaccac gtgactggca gcgactcatc aacaacaact
     2941 ggggattccg gcctaagcga ctcaacttca agctcttcaa cattcaggtc aaagaggtta
     3001 cggacaacaa tggagtcaag accatcgcca ataaccttac cagcacggtc caggtcttca
     3061 cggactcaga ctatcagctc ccgtacgtgc tcgggtcggc tcacgagggc tgcctcccgc
     3121 cgttcccagc ggacgttttc atgattcctc agtacgggta tctgacgctt aatgatggaa
     3181 gccaggccgt gggtcgttcg tccttttact gcctggaata tttcccgtcg caaatgctaa
     3241 gaacgggtaa caacttccag ttcagctacg agtttgagaa cgtacctttc catagcagct
     3301 acgctcacag ccaaagcctg gaccgactaa tgaatccact catcgaccaa tacttgtact
     3361 atctctcaaa gactattaac ggttctggac agaatcaaca aacgctaaaa ttcagtgtgg
     3421 ccggacccag caacatggct gtccagggaa gaaactacat acctggaccc agctaccgac
     3481 aacaacgtgt ctcaaccact gtgactcaaa acaacaacag cgaatttgct tggcctggag
     3541 cttcttcttg ggctctcaat ggacgtaata gcttgatgaa tcctggacct gctatggcca
     3601 gccacaaaga aggagaggac cgtttctttc ctttgtctgg atctttaatt tttggcaaac
     3661 aaggaactgg aagagacaac gtggatgcgg acaaagtcat gataaccaac gaagaagaaa
     3721 ttaaaactac taacccggta gcaacggagt cctatggaca agtggccaca aaccaccaga
     3781 acgcggtggg ggccctcagc accggctggg ttcaaaacca aggaatactt ccgggtatgg
     3841 tttggcagga cagagatgtg tacctgcaag gacccatttg ggccaaaatt cctcacacgg
     3901 acggcaactt tcacccttct ccgctgatgg gagggtttgg aatgaagcac ccgcctcctc
     3961 agatcctcat caaaaacaca cctgtacctg cggatcctcc aacggccttc aacaaggaca
     4021 agctgaactc tttcatcacc cagtattcta ctggccaagt cagcgtggag atcgagtggg
     4081 agctgcagaa ggaaaacagc aagcgctgga acccggagat ccagtacact tccaactatt
     4141 acaagtctaa taatgttgaa tttgctgtta atactgaagg tgtatatagt gaaccccgcc
     4201 ccattggcac cagatacctg actcgtaatc tgtaattgct tgttaatcaa taaaccgttt
     4261 aattcgtttc agttgaactt tggtctagag atatcagcaa aaggccagga accgtaaaaa
     4321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4741 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5161 tgcctggctc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6061 gcgcacattt ccccgaaaag tgccacct
//
