LOCUS       sequence-392599-        9399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CloneSelect C-to-T reporter encoding three target barcodes (3-input 
            OR gate circuit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9399)
  AUTHORS   Ishiguro S, Ishida K, Sakata RC, Ichiraku M, Takimoto R, Yogo R, 
            Kijima Y, Mori H, Tanaka M, King S, Tarumoto S, Tsujimura T, Bashth 
            O, Masuyama N, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, 
            Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, 
            Yamamoto T, Shakiba N, Takashima Y, Yachie N
  TITLE     A multi-kingdom genetic barcoding system for precise clone 
            isolation.
  JOURNAL   Nat Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1.
  PUBMED    40399693
REFERENCE   2  (bases 1 to 9399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1."
COMMENT     Sequence Label: pLV-CS-117
FEATURES             Location/Qualifiers
     source          1..9399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             75..255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    302..427
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    920..1153
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1338..1382
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1862..3040
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2093..3031
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2988..3008
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3053..3652
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3053..3072)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3549..3569
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3668..4256
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3721..3741)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4139..4150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        4369..4747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4748..4951
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(5071..5073,5074..5787)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5071..5073,5074..5784)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5113..5134)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5374..5393)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5721..5742
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5812..5992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6070..6191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6107..6126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6161..6180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6231..6366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6386..6405)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6387..6405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6415..6432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6415..6431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6424..6446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6573..7028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6660..6679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6870..6891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7054..7158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7159..7227,7228..8019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7377..7396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8190..8778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8679..8698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8932..8949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9066..9087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9102..9107,9108..9125,9126..9132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9140..9156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9145..9167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9164..9180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9164..9180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9199..9219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9201..9219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9247..9399,1..74)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
       61 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      241 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      301 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      361 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      421 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      481 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      541 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
      601 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
      661 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
      721 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
      781 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
      841 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
      901 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
      961 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1021 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1081 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1141 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1201 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1261 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1321 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1381 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1441 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1501 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccaagc
     1561 ttggcgtaac tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccactttggc gccggctcga gggggcccgg gtgcaaagat ggataaagtt
     1801 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     1861 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     1921 gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     1981 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2041 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     2101 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     2161 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     2221 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     2281 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     2341 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     2401 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     2461 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     2521 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     2581 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     2641 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     2701 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     2761 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     2821 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     2881 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     2941 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     3001 agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacggcca ccatgaccga
     3061 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     3121 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3181 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3241 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     3301 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     3361 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     3421 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     3481 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     3541 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     3601 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
     3661 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3721 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3781 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3841 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3901 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3961 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     4021 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     4081 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4141 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4201 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     4261 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4321 gggggactgg aagggctaat tcactcccaa cgaagacaag atggtaccac attgattatt
     4381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     4501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     4561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     4621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     4681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     4741 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     4801 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4861 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     4921 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     4981 ctggcaccgg tagccgtgag agcgtgtcag tgtgtggtgt ccgagcgtgt cagggtgacc
     5041 gtggtgtccg agtctgtctc tcacagcgtg gtgagcaagg gcgaggagct gttcaccggg
     5101 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5161 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5221 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5281 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5341 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5401 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5461 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5521 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5581 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5641 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5701 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5761 ctcggcatgg acgagctgta caagtaagaa ttcctgcttt ttgcttgtac tgggtctctc
     5821 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     5881 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     5941 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     6001 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6061 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6181 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6241 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6301 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6361 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6541 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6781 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6841 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6901 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6961 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7021 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9001 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9061 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9181 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     9241 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     9301 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     9361 acgatcgtgc cttattagga aggcaacaga cgggtctga
//
