LOCUS       sequence-392603-        8401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CloneSelect Indel reporter encoding BC-del1 barcode.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Ishiguro S, Ishida K, Sakata RC, Ichiraku M, Takimoto R, Yogo R, 
            Kijima Y, Mori H, Tanaka M, King S, Tarumoto S, Tsujimura T, Bashth 
            O, Masuyama N, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, 
            Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, 
            Yamamoto T, Shakiba N, Takashima Y, Yachie N
  TITLE     A multi-kingdom genetic barcoding system for precise clone 
            isolation.
  JOURNAL   Nat Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1.
  PUBMED    40399693
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1."
COMMENT     Sequence Label: pLV-CS-146
FEATURES             Location/Qualifiers
     source          1..8401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..39)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(279..298)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     626..647
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        708..1207
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(743..766)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1140..1159
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1227..1826
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1227..1246)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1723..1743
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1840..2428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1893..1913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2311..2322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2635..2868
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2997..3013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2997..3013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3010..3032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3021..3037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3045..3051,3052..3069,3070..3075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3090..3111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3228..3245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3399..3987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3479..3498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4158..4949,4950..5018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4781..4800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5019..5123)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5171..5305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5208..5227)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5262..5281
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5473..6106
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6153..6278
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6775..7008
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7193..7237
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7500..7615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        7673..7976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7977..8180
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8131..8151
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8177..8201
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(8377..8379,8380..8401,1..692)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8377..8379,8380..8401,1..689)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
ORIGIN
        1 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
       61 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      121 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      181 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      241 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      301 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      361 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      421 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      481 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      541 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      601 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
      661 tcactctcgg catggacgag ctgtacaagt aaggatccaa ttctaccggg taggggaggc
      721 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      781 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      841 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      901 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      961 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     1021 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     1081 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     1141 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     1201 cctttcgacc tgcagttaac gccgccatga ccgagtacaa gcccacggtg cgcctcgcca
     1261 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     1321 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     1381 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     1441 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     1501 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     1561 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     1621 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     1681 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     1741 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     1801 gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa
     1861 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     1921 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     1981 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2041 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2101 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2161 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2221 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     2281 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     2341 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     2401 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     2461 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     2521 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     2581 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     2641 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     2701 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2761 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2821 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     2881 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     2941 cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     3001 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3061 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3121 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     5161 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5221 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5281 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     5341 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     5401 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     5461 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     5521 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     5581 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     5641 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     5701 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     5761 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     5821 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     5881 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     5941 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6001 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6061 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6121 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6181 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6241 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     6301 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     6361 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     6421 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     6481 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     6541 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6601 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     6661 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     6721 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     6781 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     6841 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     6901 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     6961 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     7021 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     7081 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     7141 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     7201 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     7261 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     7321 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     7381 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     7441 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     7501 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     7561 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     7621 tttattacag ggacagcaga gatccagttt atcgataagc ttgggagttc cgcgttacat
     7681 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7741 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7801 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7861 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7921 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     7981 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8041 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8101 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8161 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     8221 gctgttttga cctccataga agacaccgat gaatgcatgt catgtaatgc tatgagagga
     8281 tctatgattt ccggtgaatt catgccgtaa gtgtcagggt catatggaag cggcagcgag
     8341 actcccggga cctcagagtc cgccacaccc gaaagtgtga gcaagggcga ggagctgttc
     8401 a
//
