LOCUS       sequence-392613-       10344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter 
            version).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Ishiguro S, Ishida K, Sakata RC, Ichiraku M, Takimoto R, Yogo R, 
            Kijima Y, Mori H, Tanaka M, King S, Tarumoto S, Tsujimura T, Bashth 
            O, Masuyama N, Adel A, Toyoshima H, Seki M, Oh JH, Archambault AS, 
            Nishida K, Kondo A, Kuhara S, Aburatani H, Klein Geltink RI, 
            Yamamoto T, Shakiba N, Takashima Y, Yachie N
  TITLE     A multi-kingdom genetic barcoding system for precise clone 
            isolation.
  JOURNAL   Nat Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1.
  PUBMED    40399693
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 May 21. doi: 10.1038/s41587-025-02649-1."
COMMENT     Sequence Label: pRS191
FEATURES             Location/Qualifiers
     source          1..10344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..33)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1168..1667
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1203..1226)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1600..1619
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1687..2286
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1687..1706)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2183..2203
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2300..2888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2353..2373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2771..2782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3095..3328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3457..3473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3457..3473)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3470..3492)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3481..3497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3505..3511,3512..3529,3530..3535))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3550..3571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3688..3705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3859..4447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3939..3958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5956..6747,6748..6816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6579..6598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6817..6921)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6969..7103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7006..7025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7060..7079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7271..7904
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7951..8076
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8573..8806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8991..9035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9298..9413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(join(9553..10266,10267..10269))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(9556..10266,10267..10269))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9598..9619)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9947..9966
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10206..10227
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(join(10325..10344,1..1158))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(10334..10344,1..928)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
ORIGIN
        1 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
       61 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
      121 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
      181 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
      241 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
      301 gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc
      361 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
      421 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
      481 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
      541 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg
      601 catttacaag actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa
      661 tggctagaga cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc
      721 ggcggcccca gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag
      781 cgcaaggcct cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt
      841 actgcagcca ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag
      901 gcccgcggga accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa
      961 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     1021 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     1081 atcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     1141 actgacgggc acggatccaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     1201 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     1261 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     1321 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     1381 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     1441 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     1501 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     1561 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     1621 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagttaac
     1681 gccgccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg
     1741 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac
     1801 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     1861 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     1921 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     1981 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2041 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2101 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2161 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2221 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2281 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2341 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2401 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2461 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2521 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2581 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2641 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     2701 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2761 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2821 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2881 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt
     2941 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     3001 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     3061 gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga
     3121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3301 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     3361 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgtttt
     3421 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3481 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3541 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3601 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3661 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3721 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3781 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3841 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3901 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3961 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4021 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4081 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4141 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4201 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4261 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4321 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4381 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4441 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4501 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4561 ttaaaaatga agttttaaat caatctaaag tactgagaga tcccctcata atttccccaa
     4621 agcgtaacca tgtgtgaata aattttgagc tagtagggtt gcagccacga gtaagtcttc
     4681 ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa ctgttgagag
     4741 tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt gtttctgagc
     4801 atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca gcatgatatc
     4861 aaaacgctct gagctgctcg ttcggctatg gcgtaggcct agtccgtagg caggactttt
     4921 caagtctcgg aaggtttctt caatctgcat tcgcttcgaa tagatattaa caagttgttt
     4981 gggtgttcga atttcaacag gtaagttagt tgctagaacc catggctcct ttgccgacgc
     5041 tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct gattttttcg
     5101 gcctttagag cgagatttat acaatagaat ttggcatgag attggattgc ttttagtcag
     5161 cctcttatag cctaaagtct ttgagtgact agatgacata tcatgtaagt tgctgatagg
     5221 tttccagttt tccgctccta ggtctgcata ttgtactttt cctcttactc gacttaacca
     5281 gtaccaaccc agcttctcaa cggatttata ccatggcact ttaaagccag catcactgac
     5341 aatgagcggt gtggtgttac tcggtagaat gctcgcaagg tcggctagaa attggtcatg
     5401 agctttcttt gaacattgct ctgaaagcgg gaacgctttc tcataaagag taacagaacg
     5461 accgtgtagt gcgactgaag ctcgcaatac cataagtcgt ttttgctcac gaatatcaga
     5521 ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag ataaagctag catgccaacg
     5581 gtatacagcg agtcgctctt tgtggaggtg acgattacct aacaatcggt cgattcgttt
     5641 gatgttatgt tttgttctcg ctttggttgg caggttacgg ccaagttcgg taagagtgag
     5701 agttttacag tcaagtaatg cgtggcaagc caacgttaag ctgttgagtc gttttaagtg
     5761 taattcgggg cagaattggt aaagagagtc gtgtaaaata tcgagttcgc acatcttgtt
     5821 gtctgattat tgatttttcg cgaaaccatt tgatcatatg acaagatgtg tatccacctt
     5881 aacttaatga tttttaccaa aatcattagg ggattcatca gtctaaagta tatatgagta
     5941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     6961 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7081 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     7141 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     7201 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     7261 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     7321 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     7381 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     7441 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     7501 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     7561 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     7621 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     7681 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     7741 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7801 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7861 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7921 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7981 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8041 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8101 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8161 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8221 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8281 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8341 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8401 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     8461 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8521 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8581 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8641 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8701 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8761 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8821 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8881 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     8941 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9001 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9061 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     9121 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     9181 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     9241 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt
     9301 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     9361 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     9421 tattacaggg acagcagaga tccagtttat cgatcccggt agaattggat cctttttttt
     9481 tttttttttt tttttttttt ttgaccatgg taatactcta gactccgtgg tgggttttgt
     9541 cctttacgct ctttacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
     9601 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
     9661 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
     9721 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
     9781 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
     9841 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
     9901 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
     9961 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
    10021 gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag
    10081 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
    10141 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
    10201 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
    10261 cttgctcacc acggtcaccc tgacacgctc gggaattcac cggaaataga tcctctagta
    10321 gagttcacga cacctgaaat ggaa
//
