LOCUS       sequence-392617-        8727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human CD283 protein expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  TITLE     TREAT-AD Plasmids 2023
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST26-hTLR3-Nluc
FEATURES             Location/Qualifiers
     source          1..8727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    677..701
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3417..3441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3452..3478
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3509..4024
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4064..4083)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4108..4156
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4358..4786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4445..4464)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4655..4676
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4795..4815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4800..5129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4980..5115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5042..5061
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5196..5990
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5250..5269)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5860..5879
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6166..6287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6203..6222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6257..6276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6336..6352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6336..6352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6349..6371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6360..6376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6384..6390,6391..6408,6409..6414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6429..6450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6567..6584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6738..7326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6818..6837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7497..8288,8289..8357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8120..8139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8358..8462)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8537..8556)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gctagttaag ctagcaacaa gtttgtacaa aaaagttggc accatgagac agactttgcc
      721 ttgtatctac ttttgggggg gccttttgcc ctttgggatg ctgtgtgcat cctccaccac
      781 caagtgcact gttagccatg aagttgctga ctgcagccac ctgaagttga ctcaggtacc
      841 cgatgatcta cccacaaaca taacagtgtt gaaccttacc cataatcaac tcagaagatt
      901 accagccgcc aacttcacaa ggtatagcca gctaactagc ttggatgtag gatttaacac
      961 catctcaaaa ctggagccag aattgtgcca gaaacttccc atgttaaaag ttttgaacct
     1021 ccagcacaat gagctatctc aactttctga taaaaccttt gccttctgca cgaatttgac
     1081 tgaactccat ctcatgtcca actcaatcca gaaaattaaa aataatccct ttgtcaagca
     1141 gaagaattta atcacattag atctgtctca taatggcttg tcatctacaa aattaggaac
     1201 tcaggttcag ctggaaaatc tccaagagct tctattatca aacaataaaa ttcaagcgct
     1261 aaaaagtgaa gaactggata tctttgccaa ttcatcttta aaaaaattag agttgtcatc
     1321 gaatcaaatt aaagagtttt ctccagggtg ttttcacgca attggaagat tatttggcct
     1381 ctttctgaac aatgtccagc tgggtcccag ccttacagag aagctatgtt tggaattagc
     1441 aaacacaagc attcggaatc tgtctctgag taacagccag ctgtccacca ccagcaatac
     1501 aactttcttg ggactaaagt ggacaaatct cactatgctc gatctttcct acaacaactt
     1561 aaatgtggtt ggtaacgatt cctttgcttg gcttccacaa ctagaatatt tcttcctaga
     1621 gtataataat atacagcatt tgttttctca ctctttgcac gggcttttca atgtgaggta
     1681 cctgaatttg aaacggtctt ttactaaaca aagtatttcc cttgcctcac tccccaagat
     1741 tgatgatttt tcttttcagt ggctaaaatg tttggagcac cttaacatgg aagataatga
     1801 tattccaggc ataaaaagca atatgttcac aggattgata aacctgaaat acttaagtct
     1861 atccaactcc tttacaagtt tgcgaacttt gacaaatgaa acatttgtat cacttgctca
     1921 ttctccctta cacatactca acctaaccaa gaataaaatc tcaaaaatag agagtgatgc
     1981 tttctcttgg ttgggccacc tagaagtact tgacctgggc cttaatgaaa ttgggcaaga
     2041 actcacaggc caggaatgga gaggtctaga aaatattttc gaaatctatc tttcctacaa
     2101 caagtacctg cagctgacta ggaactcctt tgccttggtc ccaagccttc aacgactgat
     2161 gctccgaagg gtggccctta aaaatgtgga tagctctcct tcaccattcc agcctcttcg
     2221 taacttgacc attctggatc taagcaacaa caacatagcc aacataaatg atgacatgtt
     2281 ggagggtctt gagaaactag aaattctcga tttgcagcat aacaacttag cacggctctg
     2341 gaaacacgca aaccctggtg gtcccattta tttcctaaag ggtctgtctc acctccacat
     2401 ccttaacttg gagtccaacg gctttgacga gatcccagtt gaggtcttca aggatttatt
     2461 tgaactaaag atcatcgatt taggattgaa taatttaaac acacttccag catctgtctt
     2521 taataatcag gtgtctctaa agtcattgaa ccttcagaag aatctcataa catccgttga
     2581 gaagaaggtt ttcgggccag ctttcaggaa cctgactgag ttagatatgc gctttaatcc
     2641 ctttgattgc acgtgtgaaa gtattgcctg gtttgttaat tggattaacg agacccatac
     2701 caacatccct gagctgtcaa gccactacct ttgcaacact ccacctcact atcatgggtt
     2761 cccagtgaga ctttttgata catcatcttg caaagacagt gccccctttg aactcttttt
     2821 catgatcaat accagtatcc tgttgatttt tatctttatt gtacttctca tccactttga
     2881 gggctggagg atatcttttt attggaatgt ttcagtacat cgagttcttg gtttcaaaga
     2941 aatagacaga cagacagaac agtttgaata tgcagcatat ataattcatg cctataaaga
     3001 taaggattgg gtctgggaac atttctcttc aatggaaaag gaagaccaat ctctcaaatt
     3061 ttgtctggaa gaaagggact ttgaggcggg tgtttttgaa ctagaagcaa ttgttaacag
     3121 catcaaaaga agcagaaaaa ttatttttgt tataacacac catctattaa aagacccatt
     3181 atgcaaaaga ttcaaggtac atcatgcagt tcaacaagct attgaacaaa atctggattc
     3241 cattatattg gttttccttg aggagattcc agattataaa ctgaaccatg cactctgttt
     3301 gcgaagagga atgtttaaat ctcactgcat cttgaactgg ccagttcaga aagaacggat
     3361 aggtgccttt cgtcataaat tgcaagtagc acttggatcc aaaaactctg tacatttgcc
     3421 aactttcttg tacaaagtgg ttgatctcga gtacccatac gatgttccag attacgctgg
     3481 agggggtgga tctggagggg gtggatctat ggtcttcaca ctcgaagatt tcgttgggga
     3541 ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag
     3601 tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga
     3661 aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca
     3721 aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa
     3781 ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta
     3841 tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg
     3901 gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct
     3961 gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc
     4021 gtgaggtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga
     4081 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     4141 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4201 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4261 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4321 gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4801 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4861 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4921 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5041 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5101 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5161 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5221 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5281 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5341 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5401 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5461 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5521 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5581 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5641 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5701 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5761 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5821 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5881 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5941 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6001 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6061 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6121 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8461 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     8521 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8581 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8641 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8701 gcgatgtacg ggccagatat acgcgtt
//
