LOCUS       sequence-392631-       10176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  WWP1 in pQCXIN vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Qualls-Histed SJ, Nielsen CP, MacGurn JA
  TITLE     Lysosomal trafficking of the glucose transporter GLUT1 requires 
            sequential regulation by TXNIP and ubiquitin.
  JOURNAL   iScience. 2023 Feb 6;26(3):106150. doi: 10.1016/j.isci.2023.106150. 
            eCollection 2023 Mar 17.
  PUBMED    36890792
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-02-6"; volume: "26(3):106150. doi"; pages: " 
            10.1016/j.isci.2023.106150. eCollection 2023 Mar 17"
COMMENT     Sequence Label: WT WWP1
FEATURES             Location/Qualifiers
     source          1..10176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(5084..5658,5659..5661,5662..5670)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5251..5268)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5478..5497
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5671..6465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5725..5744)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6335..6354
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6882..7307
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7410..7432)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7568..7588)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7573..7902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7753..7888
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7924..7943
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8024..8041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8195..8783)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8275..8294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8954..9745,9746..9814))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9577..9596
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9815..9919)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9987..10005
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10105..10176,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tccggaattc catggccact gcttcaccaa ggtctgatac tagtaataac cacagtggaa
     2341 ggttgcagtt acaggtaact gtttctagtg ccaaacttaa aagaaaaaag aactggttcg
     2401 gaacagcaat atatacagaa gtagttgtag atggagaaat tacgaaaaca gcaaaatcca
     2461 gtagttcttc taatccaaaa tgggatgaac agctaactgt aaatgttacg ccacagacta
     2521 cattggaatt tcaagtttgg agccatcgca ctttaaaagc agatgcttta ttaggaaaag
     2581 caacgataga tttgaaacaa gctctgttga tacacaatag aaaattggaa agagtgaaag
     2641 aacaattaaa actttccttg gaaaacaaga atggcatagc acaaactggt gaattgacag
     2701 ttgtgcttga tggattggtg attgagcaag aaaatataac aaactgcagc tcatctccaa
     2761 ccatagaaat acaggaaaat ggtgatgcct tacatgaaaa tggagagcct tcagcaagga
     2821 caactgccag gttggctgtt gaaggcacga atggaataga taatcatgta cctacaagca
     2881 ctctagtcca aaactcatgc tgctcgtatg tagttaatgg agacaacaca ccttcatctc
     2941 cgtctcaggt tgctgccaga cccaaaaata caccagctcc aaaaccactc gcatctgagc
     3001 ctgccgatga cactgttaat ggagaatcat cctcatttgc accaactgat aatgcgtctg
     3061 tcacgggtac tccagtagtg tctgaagaaa atgccttgtc tccaaattgc actagtacta
     3121 ctgttgaaga tcctccagtt caagaaatac tgacttcctc agaaaacaat gaatgtattc
     3181 cttctaccag tgcagaattg gaatctgaag ctagaagtat attagagcct gacacctcta
     3241 attctagaag tagttctgct tttgaagcag ccaaatcaag acagccagat gggtgtatgg
     3301 atcctgtacg gcagcagtct gggaatgcca acacagaaac cttgccatca gggtgggaac
     3361 aaagaaaaga tcctcatggt agaacctatt atgtggatca taatactcga actaccacat
     3421 gggagagacc acaaccttta cctccaggtt gggaaagaag agttgatgat cgtagaagag
     3481 tttattatgt ggatcataac accagaacaa caacgtggca gcggcctacc atggaatctg
     3541 tccgaaattt tgaacagtgg caatctcagc ggaaccaatt gcagggagct atgcaacagt
     3601 ttaaccaacg atacctctat tcggcttcaa tgttagctgc agaaaatgac ccttatggac
     3661 ctttgccacc aggctgggaa aaaagagtgg attcaacaga cagggtttac tttgtgaatc
     3721 ataacacaaa aacaacccag tgggaagatc caagaactca aggcttacag aatgaagaac
     3781 ccctgccaga aggctgggaa attagatata ctcgtgaagg tgtaaggtac tttgttgatc
     3841 ataacacaag aacaacaaca ttcaaagatc ctcgcaatgg gaagtcatct gtaactaaag
     3901 gtggtccaca aattgcttat gaacgcggct ttaggtggaa gcttgctcac ttccgttatt
     3961 tgtgccagtc taatgcacta cctagtcatg taaagatcaa tgtgtcccgg cagacattgt
     4021 ttgaagattc cttccaacag attatggcat taaaacccta tgacttgagg aggcgcttat
     4081 atgtaatatt tagaggagaa gaaggacttg attatggtgg cctagcgaga gaatggtttt
     4141 tcttgctttc acatgaagtt ttgaacccaa tgtattgctt atttgagtat gcgggcaaga
     4201 acaactattg tctgcagata aatccagcat caaccattaa tccagaccat ctttcatact
     4261 tctgtttcat tggtcgtttt attgccatgg cactatttca tggaaagttt atcgatactg
     4321 gtttctcttt accattctac aagcgtatgt taagtaaaaa acttactatt aaggatttgg
     4381 aatctattga tactgaattt tataactccc ttatctggat aagagataac aacattgaag
     4441 aatgtggctt agaaatgtac ttttctgttg acatggagat tttgggaaaa gttacttcac
     4501 atgacctgaa gttgggaggt tccaatattc tggtgactga ggagaacaaa gatgaatata
     4561 ttggtttaat gacagaatgg cgtttttctc gaggagtaca agaacagacc aaagctttcc
     4621 ttgatggttt taatgaagtt gttcctcttc agtggctaca gtacttcgat gaaaaagaat
     4681 tagaggttat gttgtgtggc atgcaggagg ttgacttggc agattggcag agaaatactg
     4741 tttatcgaca ttatacaaga aacagcaagc aaatcatttg gttttggcag tttgtgaaag
     4801 agacagacaa tgaagtaaga atgcgactat tgcagttcgt cactggaacc tgccgtttac
     4861 ctctaggagg atttgctgag ctcatgggaa gtaatgggcc tcaaaagttt tgcattgaaa
     4921 aagttggcaa agacacttgg ttaccaagaa gccatacatg ttttaatcgc ttggatctac
     4981 caccatataa gagttatgaa caactaaagg aaaaacttct ttttgcaata gaagagacag
     5041 agggatttgg acaagaatga gaattccgcc cccccccccc ccccccctct ccctcccccc
     5101 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5161 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5221 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5281 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5341 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5401 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5461 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5521 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5581 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5641 gaaaaacacg atgataagct tgccacaacc atggctgaac aagatggatt gcacgcaggt
     5701 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5761 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5821 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5881 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5941 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6001 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6061 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6121 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6181 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6241 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6301 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6361 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6421 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg atcggctaga
     6481 gtcgacccgg acgggacgct cctgcgcctg atacagaacg aattgcttgc aggcatctca
     6541 tgagtgtgtc ttcccgtttt ccgcctgagg tcactgcgtg gatggagcgc tggcgcctgc
     6601 tgcgcgacgg cgagctgctc accacccact cgagggctgc agcgctgcag aggccgagtg
     6661 cagaactgct ccaaagggac ctcaaggctt tccgagggac actaggctga ctccatcgag
     6721 ccagtgtaga gataagctta tcgattagtc caatttgtta aagacaggat atcagtggtc
     6781 caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta cgagccatag
     6841 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     6901 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     6961 agaatagaga agttcagatc aaggtcagga acagatggaa cagggtcgac cctagagaac
     7021 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     7081 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     7141 agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc
     7201 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
     7261 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
     7321 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggctgc
     7381 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     7441 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     7501 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtagatc
     7561 cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     7621 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     7681 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     7741 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7801 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7861 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac tggcttaact
     7921 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7981 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     8041 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8161 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8221 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8281 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8341 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8401 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8461 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8521 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8581 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8641 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8701 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8761 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8821 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8881 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8941 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9001 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9061 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9121 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9181 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9241 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     9301 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9361 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9421 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9481 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9541 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9601 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9661 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9721 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9781 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9841 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9901 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9961 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
    10021 aattagcttg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
    10081 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
    10141 ttacggggtc attagttcat agcccatata tggagt
//
