LOCUS       sequence-392658-        4267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4267)
  TITLE     pCMV-STAT6-IRES-Neo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p2xSTAT6-Luc2P
FEATURES             Location/Qualifiers
     source          1..4267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..147
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      174..183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             180..1829
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1833..1952
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1986..2034
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(2190..2207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2361..2949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2441..2460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3149..3940,3941..4009))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4114..4162
                     /note="synthetic polyadenylation signal"
     misc_feature    4176..4267
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4216..4235
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccc gctgttgctc aatcgacttc ccaagaacag cgctgttgct
       61 caatcgactt cccaagaaca gcagggatct ggcctcggcg gccaagctta gacactagag
      121 ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca
      181 tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga
      241 ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg
      301 cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg
      361 ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt
      421 gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg
      481 ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca
      541 gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa
      601 agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct
      661 tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact
      721 tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca
      781 gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc
      841 atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg
      901 tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc
      961 gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata
     1021 agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca
     1081 tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca
     1141 aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg
     1201 gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg
     1261 cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga
     1321 cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct
     1381 acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg
     1441 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     1501 tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac
     1561 accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc
     1621 ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact
     1681 atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg
     1741 aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta
     1801 aggccaagaa gggcggcaag atcgccgtga attctcacgg cttccctccc gaggtggagg
     1861 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc
     1921 ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg cgactctaga accggtcatg
     1981 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgttcgaa
     2041 ctagatgctg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     2101 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     2161 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     3121 ggccgcaaat gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct
     3181 cagcgatctg cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta
     3241 cgattcgtga gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt
     3301 caccggcccc cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg
     3361 gtcctgctac tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa
     3421 gaagttcgcc agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat
     3481 cacgctcgtc gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca
     3541 catgatcacc catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca
     3601 gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta
     3661 ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt
     3721 gtgagtagtg tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg
     3781 cgccacatag cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag
     3841 actcaaggat cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt
     3901 gatcttcagc atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa
     3961 atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt
     4021 ttcaatatta ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta
     4081 atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat
     4141 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     4201 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     4261 tttctct
//
