LOCUS       sequence-392660-        5341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGH8 - pRAB-3::mCherry::unc-54utr
FEATURES             Location/Qualifiers
     source          1..5341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1211..1235
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2103..2127)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2897..2917)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2925..2942)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2925..2941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2934..2956)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3269..3291
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3329..3347)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3415..3519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3520..3588,3589..4380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3738..3757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4551..5139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5040..5059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5266..5288
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5285..5301
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5285..5301
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5321..5341
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atcttcagat gggagcagtg gactgtctat ttgatattct taaaatgtac tctataaaag
       61 atgaaactgt aatgaccaat tccctacata aatattttta actaattggt cggatatttg
      121 gggatcagag aagtacttgt ttttgcgata ttaataatga tatatttttc tagtaaaaaa
      181 atgttaagcg atttgtgaat aattaagttg gcttgaaaaa gattttttga tagctatgta
      241 ttttgttaat ttaaatcaga ttttaaaact ttattattga ctatttatat atattatata
      301 ttatatattt tcatggttta atcaaaatct attaaattat ttctaaaatc tctaaatatc
      361 gaattctcta aaaagcttca tcaacaaaag catttcaatt atctaacaca atgagaataa
      421 ttcaagaact gaaatagaaa gaaatctaga aaaaaaagag agtgaggtgc cctattaaat
      481 cggtgctctc ctctctaaca gaagcgccta cacgcaccca agagagctct ttttctttct
      541 tttttcggag cacacataca cgcatacact cctcagaagt gtatagagaa aatgggagaa
      601 gaagcgaaag aagacgaaga agcggttgtg atgtatgcat cgggcgtttc tatctctctc
      661 cgtgagcaac gagctagtca acccatcgat ccatttttgt gagcacacac agagagagac
      721 tcaaacacac tcacagcttc acactgagct ccaagagctc tgtagagaga agccagagta
      781 tgctttcctc gattttccta acgtcttctt cttctgcttt tttctttttc ccaacgagtt
      841 gtagtccact aacttcaatt tttcctccag agaaagagtt ttagagagac agctgataaa
      901 tacactagag aaataaaaag aatgataaaa atttgctatt ttaatcagtt ccctctcgtt
      961 tctctaagtt ttgttgttgg gttgggaaat taaaattgga aaatggaaat aacgtttttt
     1021 tctgtctaat cttgaattct ccagacattt cttatttcct tttttgttgc aagtttttct
     1081 tccttctcta atttttaatc aaagatatct cgctatgaac tttgaatttt tgatattttg
     1141 attctataaa tacataaagt aatctaaatg ctcttttaaa ataaatctac agtagcccta
     1201 ttttcagatg acaagtttgt acaaaaaagc aggcttaatg gtctcaaagg gtgaagaaga
     1261 taacatggca attattaaag agtttatgcg tttcaaggtg catatggagg gatctgtcaa
     1321 tgggcatgag tttgaaattg aaggtgaagg agaaggccga ccatatgagg gaacacaaac
     1381 cgcaaaacta aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     1441 tcaggtaact aaaggcggac cattaccatt cgcctgggac atcctctctc cacagttcat
     1501 gtatggaagt aaagcttatg ttaaacatcc ggcagatata ccagattatt tgaaactttc
     1561 attcccggag ggttttaagt gggaacgcgt aatgaatttt gaagacggag gagttgttac
     1621 agtgacgcaa gactcaaggt aagtttaaac agttcggtac taactaacca tacatattta
     1681 aattttcagc ctccaagatg gagaatttat ttataaagtc aaacttcgag gaacgaattt
     1741 cccctcggat ggacctgtta tgcagaagaa gactatggga tgggaagctt caagtgaaag
     1801 aatgtaccct gaagacggtg ctcttaaggg agagattaaa caacgtctta aattgaaaga
     1861 tggaggacat tacgatgctg aggtaagttt aaacatgatt ttactaacta actaatctga
     1921 tttaaatttt caggtgaaga caacttacaa agccaaaaaa ccagttcagc tgccaggagc
     1981 gtacaatgtt aatattaaac tggatatcac ctcccacaac gaggattaca ctatcgttga
     2041 gcaatatgaa agagctgaag ggcggcactc gacaggtggc atggatgaat tgtataagta
     2101 gtacccagct ttcttgtaca aagtgggtga tatctgagct ccgcatcggc cgctgtcatc
     2161 agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct
     2221 acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaacttctct
     2281 taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt gtgtagattc
     2341 aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa
     2401 taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg ttttttactt
     2461 ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     2521 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     2581 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     2641 agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt gtttgtcaag
     2701 atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa
     2761 agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt tgataatttg
     2821 aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt cccaatatac
     2881 caaacataac tgtttccaac tttattatac atagttgata attcactggc cgtcgtttta
     2941 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3001 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3061 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3121 atttcacacc gcatatggtg cactctcagt gcaatctgct ctgatgccgc atagttaagc
     3181 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3241 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3301 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3361 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3481 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3541 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3661 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3721 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3841 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3901 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4081 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4141 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4201 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4261 gggtcagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4381 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4441 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4501 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4561 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4621 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4681 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4741 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4801 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4861 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4921 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4981 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5041 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5101 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5161 ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5221 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg ataacaattt
     5281 cacacaggaa acagctatga ccatgattac gccaagctat caactttgta tagaaaagtt
     5341 g
//
