LOCUS       sequence-392674-        5027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged human ciBAR2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Li FQ, Chen X, Fisher C, Siller SS, Zelikman K, Kuriyama R, Takemaru
            KI
  TITLE     BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To 
            Facilitate Ciliogenesis.
  JOURNAL   Mol Cell Biol. 2016 Oct 13;36(21):2668-2680. doi: 
            10.1128/MCB.00160-16. Print 2016 Nov 1.
  PUBMED    27528616
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00160-16"; journalName: "Mol Cell Biol"; date: 
            "2016-10-13- 13"; volume: "36"; issue: "21"; pages: "2668-2680"
COMMENT     Sequence Label: Flag-ciBAR2
FEATURES             Location/Qualifiers
     source          1..5027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(555..1346,1347..1415))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1178..1197
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1416..1520)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1546..2001)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1683..1704)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1895..1914
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2072..3056
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3091..3109
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3091..3108
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             3147..3170
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4135..4269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4159..4178)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4213..4232
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4382..4402)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4382..4400)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4421..4437)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4421..4437)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4434..4456)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4445..4461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4469..4475,4476..4493,4494..4499))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4514..4535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4652..4669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4823..5027,1..384))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4903..4922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
       61 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      121 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      181 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      241 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      301 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      361 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      421 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      481 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      541 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      601 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      661 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
      721 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      781 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      841 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      901 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      961 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1021 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1081 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1141 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1201 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1261 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1321 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1381 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     1561 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     1621 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     1681 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     1741 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     1801 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     1861 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     1921 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     1981 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     2041 gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc caattcaata tggcgtatat
     2101 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
     2161 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
     2221 ggcaccatgc caattcaata tggcggacct ggcactgtgc caactgggga ggggtctact
     2281 tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat
     2341 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
     2401 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
     2461 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
     2521 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
     2581 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg gtcctattga
     2641 cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct ggctcaatgc
     2701 ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca ttgcccattc
     2761 atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac ttggcagtac
     2821 atcaatatct attaatagta acttggcaag tacattacta ttggaagtac gccagggtac
     2881 attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc tgccaagtac
     2941 atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca ttgacgtaaa
     3001 tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta gggaaccgcc
     3061 attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa tacaagctac
     3121 ttgttctttt tgcaggatcc accatggatt acaaggatga cgacgataag atctcgaatt
     3181 caatgaacat cgtcttctcc agggacagcc aggtgagggt gatggagaat accgtggcca
     3241 acaccgagaa gtactttggg cagttctgct cgctgctggc cgcctacacg cgcaagacgg
     3301 cccggctgcg ggacaaggcg gaccagctgg tcaagcagct catcgacttt gccaactccg
     3361 agaaccccga gctgcgggcc accatgaggg gcttcgctga ggacctggcc aaagtgcagg
     3421 attaccggca ggcccaggtc gagaggctgg agaccaaggt ggtcaacccc ctgaagctct
     3481 acggggcaca gatcaagcag acacgggctg agatcaagaa attcaaacat gtccaaaatc
     3541 atgagatcaa acaactggaa aaactggaga aactgaggca gaagtcaccc tcggatcagc
     3601 aaatgatctc ccaggcagag accagagtgc agagggccgc tgtggactcc agccgcacca
     3661 ccctccagct ggaggagact gtggatggct tccagaggca gaagctcaag gacctgcaga
     3721 aatttttttg tgactttgta actattgaga tggttttcca tgccaaagcg gtggaggtgt
     3781 attctagcgc cttccagacc ctggagaagt atgacctgga gagggatcta ctggatttta
     3841 gagccaagat gcaaggagtt tatgggcatt atgacactcg gctgcttgcc aacaccagcc
     3901 cccctccatc tgttcttcag tctctcgcca gccagggaac tctgcaggtc cagctgagta
     3961 gggcaaatga agaccctgaa catcctcatg ccaatcatgg caggtttagt ctctgtgagt
     4021 gggtggttaa ggggcagcca gcccactgtg tgtgtgggca gggtgggcat ctcatgcttc
     4081 caggacattc tctctaactc gagcctctag aactatagtg agtcgtatta cgtagatcca
     4141 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4201 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4261 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4321 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc agcttttgtt
     4381 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4441 gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata aagtgtaaag
     4501 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4561 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4621 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4681 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4741 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4801 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4861 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4921 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4981 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgc
//
