LOCUS       sequence-392675-        4971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged human ciBAR1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Li FQ, Chen X, Fisher C, Siller SS, Zelikman K, Kuriyama R, Takemaru
            KI
  TITLE     BAR Domain-Containing FAM92 Proteins Interact with Chibby1 To 
            Facilitate Ciliogenesis.
  JOURNAL   Mol Cell Biol. 2016 Oct 13;36(21):2668-2680. doi: 
            10.1128/MCB.00160-16. Print 2016 Nov 1.
  PUBMED    27528616
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00160-16"; journalName: "Mol Cell Biol"; date: 
            "2016-10-13- 13"; volume: "36"; issue: "21"; pages: "2668-2680"
COMMENT     Sequence Label: Flag-ciBAR1
FEATURES             Location/Qualifiers
     source          1..4971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(559..1350,1351..1419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1182..1201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1420..1524)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1550..2005)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1687..1708)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1899..1918
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2076..3060
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3095..3113
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3095..3112
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             3151..3174
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    4003..4014
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4083..4217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4107..4126)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4161..4180
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4330..4350)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4330..4348)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4369..4385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4369..4385)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4382..4404)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4393..4409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4417..4423,4424..4441,4442..4447))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4462..4483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4600..4617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(4771..4971,1..388))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4851..4870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
       61 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
      601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
      661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
      721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
      781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
      841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
      901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
      961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     1441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     1501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     1561 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1621 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1681 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1741 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1801 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1861 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1921 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1981 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     2041 gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccgccaattc aatatggcgt
     2101 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
     2161 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
     2221 acttggcacc atgccaattc aatatggcgg acctggcact gtgccaactg gggaggggtc
     2281 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
     2341 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
     2401 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
     2461 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
     2521 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
     2581 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
     2641 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
     2701 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
     2761 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
     2821 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg
     2881 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
     2941 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
     3001 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
     3061 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
     3121 ctacttgttc tttttgcagg atccaccatg gattacaagg atgacgacga taagatctcg
     3181 aattcaatga ggcgcaccct ggaaaaccgg aacgctcaaa cgaaacaact gcaaacagct
     3241 gtctcaaatg tggagaagca ttttggagaa ctgtgccaaa tcttcgctgc ctatgtgcgg
     3301 aaaactgcca ggctgagaga caaagcagac ctcctggtga atgaaattaa cgcgtatgct
     3361 gctacagaga ccccgcattt aaagctgggc ctgatgaact ttgcagatga gtttgccaaa
     3421 cttcaggatt atcgacaagc agaggttgaa agacttgaag ccaaagtagt tgaacccttg
     3481 aaaacttatg ggaccattgt gaaaatgaaa cgggatgacc tcaaagcaac actcacagca
     3541 aggaatcgag aagctaagca attaactcag ttagaaagaa cacgtcagcg aaacccatct
     3601 gatcgacatg ttatttcaca ggcagaaacg gaattacaga gagctgcaat ggatgctagc
     3661 cgaacaagtc gtcatctgga ggaaactatt aacaactttg aaaggcagaa aatgaaggat
     3721 ataaagacta tattttctga atttatcaca atcgaaatgt tatttcacgg caaagcttta
     3781 gaggtctaca ctgctgccta ccagaatata caaaacattg atgaagatga agatttagag
     3841 gttttccgaa attctctgta tgcaccagat tattcatctc gtttagatat tgtaagagca
     3901 aattcaaagt cacctcttca gagatcactg tcagctaagt gtgtatctgg aacaggacag
     3961 gtatccactt gtcgactaag aaaggatcaa caagcagaag atgatgagga tgacgagtta
     4021 gatgttacag aagaagaaaa ttttcttaag taatctagaa ctatagtgag tcgtattacg
     4081 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4141 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4201 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4261 aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag
     4321 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     4381 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     4441 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4501 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4561 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4621 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct c
//
