LOCUS       sequence-392692-        9961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2495
FEATURES             Location/Qualifiers
     source          1..9961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2899..3487
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2952..2972)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3370..3381)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3559..3792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3870..3991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3907..3926)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3961..3980
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4031..4166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4186..4205)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4187..4205)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4215..4232)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4215..4231)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4224..4246)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4373..4828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4460..4479)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4670..4691
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4854..4958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4959..5027,5028..5819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5177..5196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6479..6498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6732..6749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6866..6887
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6902..6907,6908..6925,6926..6932)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6940..6956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6945..6967
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6964..6980
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6964..6980
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6999..7019
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7001..7019
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7047..7273
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7274..7454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7501..7626
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8119..8352
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8537..8581
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8812..9022
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8834..9030
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8881..9016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9062..9664
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9062..9081)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9558..9578
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9707..9824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtg
     1201 tcctaaaggt tatgaagaca gtatggagtt tccagaccat agtagacatt tgctacagtg
     1261 tctgagcgag cagagacacc agggttttct ttgtgactgc actgttctgg tgggagatgc
     1321 ccagttccga gcgcaccgag ctgtactggc ttcatgcagc atgtatttcc acctctttta
     1381 caaggaccag ctggacaaaa gagacattgt tcatctgaac agcgacattg ttacagcccc
     1441 cgctttcgct ctcctgcttg aattcatgta tgaagggaaa ctccagttca aagacttgcc
     1501 cattgaagac gtgctagcag ctgccagtta tctccacatg tatgacattg tcaaagtctg
     1561 caaaaagaag ctgaaagaga aagccaccac ggaggcagac agcaccaaaa aggaagaaga
     1621 tgcttcaagt tgttcggaca aagtcgagag tctctccgat ggcagcagcc acatagcagg
     1681 cgatttgccc agtgatgaag atgaaggaga agatgaaaaa ttgaacatcc tgcccagcaa
     1741 aagggacttg gcggccgagc ctgggaacat gtggatgcga ttgccctcag actcagcagg
     1801 catcccccag gctggcggag aggcagagcc acacgccaca gcagctggaa aaacagtagc
     1861 cagcccctgc agctcaacag agtctttgtc ccagaggtct gtcacctccg tgagggattc
     1921 ggcagatgtt gactgtgtgc tggacctgtc tgtcaagtcc agcctttcag gagttgaaaa
     1981 tctgaacagc tcttatttct cttcacagga cgtgctgaga agcaacctgg tgcaggtgaa
     2041 ggtggagaaa gaggcttcct gtgatgagag tgatgttggc actaatgact atgacatgga
     2101 acatagcact gtgaaagaaa gtgtgagcac taataacagg gtacagtatg agccggccca
     2161 tctggctccc ctgagggagg actcggtctt gagggagctg gaccgggagg acaaagccag
     2221 tgatgatgag atgatgaccc cagagagcga gcgtgtccag gtggagggag gcatggagag
     2281 cagtctgctc ccctacgtct ccaacatcct gagccccgcg ggccagatct tcatgtgccc
     2341 cctgtgcaac aaggtcttcc ccagccccca catcctgcag atccacctga gcacgcactt
     2401 ccgcgagcag gacggcatcc gcagcaagcc cgccgccgat gtcaacgtgc ccacgtgctc
     2461 gctgtgtggg aagactttct cttgcatgta caccctcaag cgccacgaga ggactcactc
     2521 gggggagaag ccctacacat gcacccagtg cggcaagagc ttccagtact cgcacaacct
     2581 gagccgccat gccgtggtgc acacccgcga gaagccgcac gcctgcaagt ggtgcgagcg
     2641 caggttcacg cagtccgggg acctgtacag acacattcgc aagttccact gtgagttggt
     2701 gaactccttg tcggtcaaaa gcgaagcact gagcttgcct actgtcagag actggacctt
     2761 agaagatagc tctcaagaac tttggaaata gtaatgaact agtccgtaac ttgaaagtat
     2821 ttcgatttct tggctttata tatcttgtgg aaaggacgaa ataaacatgt ttaactcttc
     2881 ggcacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3301 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3481 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3541 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3601 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3661 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3721 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3781 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3841 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3901 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3961 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4081 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4141 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4201 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4261 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4321 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4441 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4501 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4561 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4681 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4741 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4801 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4861 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4921 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6781 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6841 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6901 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6961 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7021 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7081 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7141 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7201 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7261 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7321 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7381 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7441 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7501 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7561 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7621 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7681 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7741 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7801 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7861 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7921 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7981 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8041 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8101 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8161 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8221 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8281 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8341 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8401 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8461 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8521 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8581 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8641 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8701 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     8761 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8821 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     8881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8941 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9001 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9061 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9241 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9661 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9721 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9781 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9841 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
     9901 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     9961 t
//
