LOCUS       sequence-392697-        8908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0152
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1846..2434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1899..1919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2317..2328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2506..2739
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2817..2938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2854..2873)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2908..2927
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2978..3113
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3133..3152)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3134..3152)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3162..3179)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3162..3178)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3171..3193)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3320..3775
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3407..3426)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3617..3638
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3801..3905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3906..3974,3975..4766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4124..4143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4937..5525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5426..5445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5679..5696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5813..5834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5849..5854,5855..5872,5873..5879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5887..5903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5892..5914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5911..5927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5911..5927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5946..5966
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5948..5966
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5994..6220
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6221..6401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6448..6573
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7066..7299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7484..7528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7759..7969
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7781..7977
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7828..7963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8009..8611
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8009..8028)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8505..8525
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8654..8771
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 gaaatgctgc tccgccattg gatgtgcttc tcgctgcttg ccaaattcga agttaaaagg
     1261 actgacattt cacgtattcc ccacagatga aaacatcaaa aggaaatggg tattagcaat
     1321 gaaaagactt gatgtgaatg cagccggcat ttgggagcct aaaaaaggag atgtgttgtg
     1381 ttcgaggcac tttaagaaga cagattttga cagaagtgct ccaaatatta aactgaaacc
     1441 tggagtcata ccttctatct ttgattctcc atatcaccta caggaacata gctacagtgt
     1501 aatggacagt ccaaagaaac ttaagcataa attagatcat gtgatcggcg agctagagga
     1561 tacaaaggaa agtctacgga atgttttaga ccgagaaaaa cgttttcaga aatcattgag
     1621 gaagacaatc agggaattaa aggatgaatg tctgatcagc caagaaacag caaatagact
     1681 ggacactttc tgttgggact gttgtcagga gagcatagaa caggactata tttcatagta
     1741 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     1801 ggacgatagc aagcgaaccg tagaataatt acgcgttaag tcgacaatca acctctggat
     1861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     1921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     1981 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2041 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2101 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2161 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2221 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     2281 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     2341 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     2401 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     2461 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     2521 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     2581 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2641 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2701 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     2761 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     2821 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2881 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     2941 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     3001 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3061 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     3121 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     3181 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     3241 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     3301 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3361 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3421 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3481 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3541 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3601 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3661 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3721 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     3781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     4141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     4201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     5101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5761 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5821 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5881 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     5941 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     6001 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     6061 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     6121 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     6181 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     6241 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6301 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6361 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     6421 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     6481 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     6541 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     6601 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     6661 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     6721 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     6781 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     6841 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     6901 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     6961 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     7021 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     7081 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     7141 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     7201 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     7261 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     7321 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     7381 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     7441 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     7501 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     7561 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     7621 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     7681 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     7741 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     7801 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7861 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7921 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7981 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     8041 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     8101 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     8161 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     8221 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     8281 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     8341 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     8401 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     8461 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     8521 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     8581 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
     8641 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     8701 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     8761 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     8821 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     8881 cttctaggtc ttgaaaggag tgggaatt
//
