LOCUS       sequence-392709-       11332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express APOBEC1 cytosine deaminase fused with YTH m6A binding domain
            from UbiC promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11332)
  TITLE     Hart Lab Plasmids
REFERENCE   2  (bases 1 to 11332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-APOBEC1-YTH-mCherry
FEATURES             Location/Qualifiers
     source          1..11332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      183..318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     245..264
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        380..427
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             446..820
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    950..1071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(987..1006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1041..1060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1120..1136)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1120..1136)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1133..1155)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1144..1160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1168..1174,1175..1192,1193..1198))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1213..1234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1351..1368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1522..2110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1602..1621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2281..3072,3073..3141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2904..2923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3142..3246)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3321..3340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3512..3891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3893..4091
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4046..4066
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4109..4289
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4336..4461
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4954..5187
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5372..5416
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5714..5831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5896..7107
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     6944..6963
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7145..7831
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             8462..8515
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8528..8548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8549..9259
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8694..8712)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8962..8981
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9156..9179
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9298..9886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9351..9371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9769..9780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9889..9905)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9890..9906)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10411..10591
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10617..10634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10623..10847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10893..11321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10980..10999)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11190..11211
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(11330..11332,1..18))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
       61 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      121 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      181 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      241 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      301 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      361 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      421 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      481 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      541 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      601 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      661 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      721 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      781 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      841 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      901 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
      961 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1021 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1081 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1141 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1201 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1261 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1321 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1381 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1441 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1921 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2581 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2881 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3241 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3301 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3361 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3421 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3481 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3541 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3901 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3961 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4021 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4081 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4261 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4321 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4381 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4441 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4501 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4561 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4621 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4681 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4741 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4801 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4861 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4921 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     4981 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5041 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5101 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5161 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5221 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5281 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5341 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5401 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5461 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5521 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5581 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5641 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5701 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5761 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5821 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaacccgt
     5881 gtcggctcca gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     5941 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     6001 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     6061 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     6121 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     6181 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     6241 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     6301 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     6361 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     6421 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     6481 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     6541 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     6601 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     6661 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     6721 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     6781 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     6841 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     6901 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     6961 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     7021 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     7081 tggccgtttt tggctttttt gttagacgaa gcttgggctg caggtcgact ggccacccgc
     7141 caccatgagc tcagagactg gcccagtggc tgtggacccc acattgagac ggcggatcga
     7201 gccccatgag tttgaggtat tcttcgatcc gagagagctc cgcaaggaga cctgcctgct
     7261 ttacgaaatt aattgggggg gccggcactc catttggcga catacatcac agaacactaa
     7321 caagcacgtc gaagtcaact tcatcgagaa gttcacgaca gaaagatatt tctgtccgaa
     7381 cacaaggtgc agcattacct ggtttctcag ctggagccca tgcggcgaat gtagtagggc
     7441 catcactgaa ttcctgtcaa ggtatcccca cgtcactctg tttatttaca tcgcaaggct
     7501 gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg gatttgatct cttcaggtgt
     7561 gactatccaa attatgactg agcaggagtc aggatactgc tggagaaact ttgtgaatta
     7621 tagcccgagt aatgaagccc actggcctag gtatccccat ctgtgggtac gactgtacgt
     7681 tcttgaactg tactgcatca tactgggcct gcctccttgt ctcaacattc tgagaaggaa
     7741 gcagccacag ctgacattct ttaccatcgc tcttcagtct tgtcattacc agcgactgcc
     7801 cccacacatt ctctgggcca ccgggttgaa aagcggcagc gagactcccg ggacctcaga
     7861 gtccgccaca ccagaacccc acccagtgtt ggagaagctt cggtccatta ataactataa
     7921 ccccaaagat tttgactgga atctgaaaca tggccgggtt ttcatcatta agagctactc
     7981 tgaggacgat attcaccgtt ccattaagta taatatttgg tgcagcacag agcatggtaa
     8041 caagagactg gatgctgctt atcgttccat gaacgggaaa ggccccgttt acttactttt
     8101 cagtgtcaac ggcagtggac acttctgtgg cgtggcagaa atgaaatctg ctgtggacta
     8161 caacacatgt gcaggtgtgt ggtcccagga caaatggaag ggtcgttttg atgtcaggtg
     8221 gatttttgtg aaggacgttc ccaatagcca actgcgacac attcgcctag agaacaacga
     8281 gaataaacca gtgaccaact ctagggacac tcaggaagtg cctctggaaa aggctaagca
     8341 ggtgttgaaa attatagcca gctacaagca caccacttcc atttttgatg acttctcaca
     8401 ctatgagaaa cgccaagagg aagaagaaag tgttaaaaag gaacgtcaag gtcgtgggaa
     8461 agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gccctctaga
     8521 gatggctcca aagaagaagc gtaaggtaat ggtgagcaag ggcgaggagg acaacatggc
     8581 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccgcga
     8641 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     8701 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     8761 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     8821 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     8881 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     8941 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     9001 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     9061 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacaagg ccaagaagcc
     9121 cgtgcagctg cccggcgcct acaacgtcaa catcaagctg gacatcacct cccacaacga
     9181 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcggcat
     9241 ggacgagctg tacaagtaaa ttaagctagt ctagaattcg atatcaagct tatcgataat
     9301 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     9361 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     9421 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     9481 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     9541 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     9601 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     9661 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     9721 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9781 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9841 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     9901 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     9961 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
    10021 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    10081 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
    10141 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
    10201 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
    10261 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
    10321 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
    10381 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
    10441 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10501 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10561 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
    10621 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    10681 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    10741 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    10801 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    10861 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
    10921 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    10981 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    11041 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    11101 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    11161 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    11221 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    11281 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg ga
//
