LOCUS       sequence-392710-        9704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GCaMP8f from hSyn1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  TITLE     Hart Lab Plasmids
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FSW_jGCaMP8f
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             465..482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     764..785
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(891..912)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1152..1171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1750..2338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1803..1823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2221..2232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2341..2357)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2342..2358)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2863..3043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3069..3086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3075..3299
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3345..3773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3432..3451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3642..3663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3782..3802)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3787..4116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3967..4102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4029..4048
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4164..4211
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4230..4604
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4734..4855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4771..4790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4825..4844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4904..4920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4904..4920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4917..4939)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4928..4944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4952..4958,4959..4976,4977..4982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4997..5018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5135..5152)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5306..5894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5386..5405)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6065..6856,6857..6925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6688..6707
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6926..7030)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7105..7124)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7296..7675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7677..7875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7830..7850
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7893..8073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8120..8245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8738..8971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9156..9200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9498..9615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9681..9704,1..424)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc
       61 aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg
      121 gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg
      181 cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc
      241 gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc
      301 gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg
      361 cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc
      421 gcagtcgaat tcaagctgct agcaaggatc cacccgccac catgcatcat caccatcatc
      481 acacgcgtcg caagaagacc ttcaaggagg tggccaccgc cgtgaagatc atcgcccgcc
      541 tgatgggcct gaagatcaac gtctatatca aggccgacaa gcagaagaac ggcatcaagg
      601 cgaacttcca catccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc
      661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg
      721 tggagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      781 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag
      841 ggagcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      901 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca
      961 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1021 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1081 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca
     1141 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1201 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1261 ggcacaagct ggagtacaac ctgccggacc aactgactga agagcagatc gcagaattta
     1321 aagaggcttt ctccctattt gacaaggacg gggatgggac aataacaacc aaggagctgg
     1381 ggacggtgat gcggtctctc ggactgaacc ccacagaagc agagctgcag gacatgatca
     1441 atgaagtaga tgccgacggt gacggcacaa tcgacttccc tgagttcctg acaatgatgg
     1501 caagaaaaat gaaatacagg gacacggaag aagaaattag agaagcgttc ggtgtgtttg
     1561 ataaggatgg caatggctac atcagtgcag cagagcttcg ccacgtgatg acaaaccttg
     1621 gagagaagtt aacagatgaa gaggttgatg aaatgatcag ggaagcagac atcgatgggg
     1681 atggtcaggt aaactacgaa gagtttgtac aaatgatgac agcgaagtga cgatatcaag
     1741 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     1801 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     1861 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     1921 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     1981 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2041 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2101 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2161 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2221 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2281 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     2341 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     2401 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     2461 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2521 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     2581 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     2641 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     2701 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     2761 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     2821 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     2881 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2941 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3001 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     3061 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     3121 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     3181 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     3241 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     3301 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     3361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     3781 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3841 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3901 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4021 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     4081 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     4141 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     4201 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     4261 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     4321 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     4381 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     4441 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     4501 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     4561 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     4621 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4681 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     4741 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4801 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4861 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     4921 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4981 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5041 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6361 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6661 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7021 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7081 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7141 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     7201 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     7261 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     7321 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     7381 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     7441 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     7501 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     7561 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     7621 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     7681 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     7741 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     7801 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     7861 ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag
     7921 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7981 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctta
     8041 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8101 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8161 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8221 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8281 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8341 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8401 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8461 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8521 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8581 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8641 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8701 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     8761 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     8821 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     8881 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     8941 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9001 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9061 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9121 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9181 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9241 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9301 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9361 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     9421 acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg
     9481 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     9541 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     9601 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gttaattaac
     9661 tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccag
//
