LOCUS       sequence-392713-        9571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0233
FEATURES             Location/Qualifiers
     source          1..9571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2509..3097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2562..2582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2980..2991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3169..3402
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3480..3601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3517..3536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3571..3590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3641..3776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3796..3815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3797..3815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3825..3842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3825..3841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3834..3856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3983..4438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4070..4089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4280..4301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4464..4568
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4569..4637,4638..5429)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4787..4806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5600..6188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6089..6108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6342..6359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6476..6497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6512..6517,6518..6535,6536..6542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6550..6566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6555..6577
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6574..6590
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6574..6590
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6609..6629
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6611..6629
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6657..6883
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6884..7064
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7111..7236
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7729..7962
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8147..8191
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8422..8632
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8444..8640
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8491..8626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8672..9274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8672..8691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9168..9188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9317..9434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 aaaatccaaa aatttccgca tcgacgccct gctggcggtg gaccccccac gagccgcctc
     1261 tgcgcagagc gcgccgctgg ccttggtcac gtcgctcgcc gccgccgcat ctggcaccgg
     1321 aggtggcggc ggcggcggcg gggcgagcgg cgggactagc ggcagctgca gccccgcgtc
     1381 ctcggagccg ccggctgcgc ccgccgaccg cctgcgcgcc gagagcccgt cgccgccgcg
     1441 cctgctggcc gcgcactgcg cgctgctgcc caagccgggc ttcctgggcg cgggcggcgg
     1501 cggcggcggc acgggcggcg ggcacggggg gccccaccac cacgcgcatc cgggcgcagc
     1561 ggccgctgcc gccgccgccg ccgccgccgc cgccgctggg ggcctggcgc tggggctgca
     1621 ccctgggggc gcgcagggcg gcgcgggcct cccggcgcag gcggcgctct acggccaccc
     1681 ggtctacggc tactccgcgg cggcggcggc ggctgcgctg gcgggccagc acccggcgct
     1741 ctcctactcg tacccgcagg tgcaaggcgc gcaccccgcg caccccgccg accccatcaa
     1801 gctgggcgcc ggcaccttcc agctggacca gtggctgcgc gcgtccaccg cgggcatgat
     1861 cctgcctaag atgcccgact tcaactccca ggcgcagtcg aacctcctgg ggaagtgccg
     1921 ccggccgcgc accgccttca ccagccagca gctgctggag ctggagcacc agttcaagct
     1981 caacaagtac ctgtcgcggc ccaagcgctt cgaggtggcc acctcgctca tgctcaccga
     2041 gacccaggtg aagatttggt tccagaaccg gcggatgaaa tggaaacgca gcaaaaaggc
     2101 caaagagcag gcggcgcagg aagcggagaa acagaagggc ggcggcgggg gcgcggggaa
     2161 gggcggcgcg gaggagccgg gagccgagga gctgctgggg ccgccagcgc ccggagacaa
     2221 gggcagcgga cgccgcctgc gggacttgag ggacagtgac cccgaggagg acgaggacga
     2281 ggacgacgag gaccatttcc cctacagcaa cggcgccagc gtccacgccg cctcctccga
     2341 ctgctcctcg gaggacgact cgccgccccc gcggcccagc caccagcccg cgccccagta
     2401 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2461 aaaggacgat gcgttcgcta atccgatagc gccacgcgtt aagtcgacaa tcaacctctg
     2521 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2581 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2641 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2701 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2761 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2821 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     2881 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     2941 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3001 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3061 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     3121 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3181 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3241 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3301 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3361 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     3421 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     3481 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3541 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3601 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     3661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3721 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3781 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     3841 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     3901 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     3961 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4021 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4081 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4141 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4201 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4261 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4321 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4381 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4441 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4501 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4561 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4621 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4681 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4741 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4801 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4861 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4921 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4981 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5041 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5101 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5161 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5221 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5281 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5341 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5401 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5461 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5521 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6421 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6481 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6541 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6601 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     6661 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     6721 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     6781 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     6841 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     6901 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6961 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7021 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7081 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7141 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7201 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7261 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7321 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7381 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7441 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7501 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7561 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     7621 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     7681 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     7741 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     7801 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     7861 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     7921 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     7981 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8041 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8101 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8161 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8221 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8281 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8341 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     8401 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     8461 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8641 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
     8701 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     8761 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     8821 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     8881 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     8941 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     9001 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     9061 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     9121 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     9181 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     9241 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
     9301 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     9361 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9421 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
     9481 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     9541 gaccttctag gtcttgaaag gagtgggaat t
//
