LOCUS       sequence-392714-        9472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0292
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2410..2998
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2463..2483)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2881..2892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3070..3303
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3381..3502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3418..3437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3472..3491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3542..3677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3697..3716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3698..3716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3726..3743)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3726..3742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3735..3757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3884..4339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3971..3990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4181..4202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4365..4469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4470..4538,4539..5330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4688..4707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5501..6089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5990..6009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6243..6260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6377..6398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6413..6418,6419..6436,6437..6443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6451..6467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6456..6478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6475..6491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6475..6491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6510..6530
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6512..6530
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6558..6784
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6785..6965
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7012..7137
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7630..7863
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8048..8092
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8323..8533
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8345..8541
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8392..8527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8573..9175
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8573..8592)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9069..9089
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9218..9335
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 gaggaaaaaa atccagatct cccgcatcct ggaccaaagg aatcggcagg tgacgttcac
     1261 caagcggaag ttcgggctga tgaagaaggc ctatgagctg agcgtgctct gtgactgtga
     1321 gatagccctc atcatcttca acagcgccaa ccgcctcttc cagtatgcca gcacggacat
     1381 ggaccgtgtg ctgctgaagt acacagagta cagcgagccc cacgagagcc gcaccaacac
     1441 tgacatcctc gagacgctga agcggagggg cattggcctc gatgggccag agctggagcc
     1501 ggatgaaggg cctgaggagc caggagagaa gtttcggagg ctggcaggcg aagggggtga
     1561 tccggccttg ccccgacccc ggctgtatcc tgcagctcct gctatgccca gcccagatgt
     1621 ggtatacggg gccttaccgc caccaggctg tgaccccagt gggcttgggg aagcactgcc
     1681 cgcccagagc cgcccatctc ccttccgacc agcagccccc aaagccgggc ccccaggcct
     1741 ggtgcaccct ctcttctcac caagccacct caccagcaag acaccacccc cactgtacct
     1801 gccgacggaa gggcggaggt cagacctgcc tggtggcctg gctgggcccc gagggggact
     1861 aaacacctcc agaagcctct acagtggcct gcagaacccc tgctccactg caactcccgg
     1921 acccccactg gggagcttcc ccttcctccc cggaggcccc ccagaatatg gcctgggaga
     1981 ccctccaccg ccccctggct tgttgcagcc ccccaccctg gccccctggc agccctcgag
     2041 gggtgatggg ccccccgccg tgtcctccca gcccagtggg ggccgaagcc tgggcgagga
     2101 gggtccccca acccgcggcg cctccccgcc gaccccccca gtcagcatca agtctgagcg
     2161 cctctctccg gcccccgggg gccccggcga ctttcctaag accttcccct atcccttgct
     2221 cctcgcccgg tccctggcag agcctctgcg gcctgggccc gccctgcgcc ggctgccctt
     2281 ggccgacggc tggccccggt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     2341 ttggctttat atatcttgtg gaaaggacga gcgtctacaa atttagcttt agtcacgcgt
     2401 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2461 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2521 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2581 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2641 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2701 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2761 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2821 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2881 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     2941 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3001 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3061 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3121 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3181 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3241 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3301 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3361 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3421 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3481 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3541 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3601 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3661 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     3721 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3901 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3961 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4021 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4081 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4141 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4201 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4261 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4321 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4381 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4441 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4501 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4561 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4621 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4681 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4741 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4801 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4861 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4921 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4981 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5041 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5101 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5161 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5221 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5281 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5341 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5401 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6181 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6241 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6301 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6361 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6421 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6481 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6541 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6601 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6661 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6721 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     6781 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6841 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6901 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6961 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7021 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7081 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7141 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7201 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7261 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7321 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7381 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7441 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7501 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7561 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7621 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7681 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7741 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7801 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7861 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7921 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     7981 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8041 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8101 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8161 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8221 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     8281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8341 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8461 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8521 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     8581 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     8641 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8701 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8761 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     8821 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8881 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8941 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     9001 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     9061 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     9121 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     9181 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     9241 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9301 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9361 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
     9421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
