LOCUS       sequence-392726-       10309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1778
FEATURES             Location/Qualifiers
     source          1..10309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3247..3835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3300..3320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3718..3729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3907..4140
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4218..4339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4255..4274)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4309..4328
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4379..4514
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4534..4553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4535..4553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4563..4580)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4563..4579)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4572..4594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4721..5176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4808..4827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5018..5039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5202..5306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5307..5375,5376..6167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5525..5544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6338..6926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6827..6846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7080..7097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7214..7235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7250..7255,7256..7273,7274..7280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7288..7304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7293..7315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7312..7328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7312..7328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7347..7367
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7349..7367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7395..7621
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7622..7802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7849..7974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8467..8700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8885..8929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9160..9370
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9182..9378
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9229..9364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9410..10012
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9410..9429)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9906..9926
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10055..10172
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 atttagcatc aagcagagtc ccctttctgt tcagagtgtt gtaaagtgca taaagatgaa
     1261 gcaggcacca gaaatcctcg gcagtgccaa cgggaagact ccgagctgcg aggtgaaccg
     1321 cgagtgttct gtgttcctca gcaaagccca gctctccagt agcctgcagg agggggtcat
     1381 gcagaagttt aacggccacg acgccctgcc ctttattcca gccgacaagc tgaaagatct
     1441 tacttcccgg gtgtttaatg gagaacccgg cgcacacgat gccaaactgc gttttgagtc
     1501 ccaggaaatg aaagggattg ggacaccccc taacactacc cctatcaaaa atggctctcc
     1561 agaaattaag ctgaaaatca ccaaaacata catgaatggg aagcctctct ttgaatcttc
     1621 catttgtggt gacagtgctg ctgatgtgtc tcagtcagaa gaaaatggac aaaaaccaga
     1681 aaacaaggcg agaaggaaca ggaagaggag cataaaatat gactccttgc tggagcaggg
     1741 ccttgtcgaa gcagctcttg tgtctaagat ctcaagtcct tcagataaaa agattccagc
     1801 taagaaagag tcttgtccaa acactggaag agacaaagac cacctgttga aatacaacgt
     1861 tggtgatttg gtgtggtcca aagtgtcggg ttacccttgg tggccttgca tggtttctgc
     1921 agatccactc cttcacagct ataccaaact taaaggtcag aaaaagagtg cacgccagta
     1981 tcacgtacag ttctttggtg acgccccaga aagagcttgg atatttgaga agagcctcgt
     2041 agcttttgaa ggagaaggac agtttgaaaa attatgccag gaaagtgcca agcaggcacc
     2101 cacgaaagct gagaaaatta agctattgaa accaatttca gggaaattga gggcccagtg
     2161 ggaaatgggc attgttcaag cagaagaagc tgcaagcatg tcagtggagg agcggaaagc
     2221 caagttcacc tttctctatg tgggggacca gcttcatctc aaccctcaag tagccaagga
     2281 ggctggcatt gctgcagagt ctttgggaga aatggcagaa tcctcaggag tcagtgaaga
     2341 agctgctgaa aaccccaagt ctgtgagaga agagtgcatt cccatgaaga gaaggcggag
     2401 ggccaaactg tgtagctctg cagagaccct ggagagtcac cccgacatag ggaagagtac
     2461 tcctcaaaag acggcagagg ctgaccccag aagaggagta gggtctcctc ctgggaggaa
     2521 gaagaccaca gtctccatgc cacgaagcag gaagggagat gcagcatccc agtttttggt
     2581 cttctgtcaa aaacacaggg atgaggtggt agctgagcac ccagatgctt caggtgagga
     2641 gattgaagag ctgctcaggt cacagtggag tctgctgagt gagaagcaga gagcacgcta
     2701 caacaccaag tttgccctgg tggcccctgt ccaggctgaa gaagactctg gtaatgtaaa
     2761 tgggaaaaaa agaaaccaca caaagaggat acaggaccct acagaagatg ctgaagctga
     2821 ggacacaccc aggaaaagac tcaggacgga caagcacagt cttcggaaga gagacacaat
     2881 cactgacaaa acggccagaa caagctctta caaggccatg gaggcagcct cctcgctcaa
     2941 gagccaggca gcaacgaaaa atctgtctga tgcatgtaaa ccactgaaga agcgaaatcg
     3001 ggcttccacg gcagcatctt cagctcttgg gtttagcaaa agttcatctc cttctgcatc
     3061 cttaactgag aatgagcttt tgtgggagcc cacaccagtc aagttggatt tgaacccagc
     3121 tgctctgtac tgcacttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3181 gctttatata tcttgtggaa aggacgactg caaggttcag ggcggatccc tacgcgttaa
     3241 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3841 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3901 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3961 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4021 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4081 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4141 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4201 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4321 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4381 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4441 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4501 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4561 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4621 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4681 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4861 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4921 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5101 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5161 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7141 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7201 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7261 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7321 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7381 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7441 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7501 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7561 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7621 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7681 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7741 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7801 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7861 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7921 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7981 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8041 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8101 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8161 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8221 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8281 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8341 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8401 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8461 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8521 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8581 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8641 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8701 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8761 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8821 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8881 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8941 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9001 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9061 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9181 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9361 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9601 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     9661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10021 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10081 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10141 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10201 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10261 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
