LOCUS       sequence-392732-       10906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_2 [pBBR1 ORI]
FEATURES             Location/Qualifiers
     source          1..10906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      172..943
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             944..1606
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     complement(1924..1940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             2100..2194
                     /estimated_length=95
     primer_bind     2234..2250
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2418..2812
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3154..3241
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4480..5451
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             5962..6801
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             6788..7639
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     complement(7964..7980)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             8045..8536
                     /estimated_length=492
     primer_bind     complement(8950..8966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(9138..9726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9218..9237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9859..9953)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9974..10633)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10548..10567
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10634..10736)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     10838..10854
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagcgccct acgggcttgc tctccgggct tcgccctgcg
      121 cggtcgctgc gctcccttgc cagcccgtgg atatgtggac gatggccgcg agcggccacc
      181 ggctggctcg cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg
      241 cgcctgccca cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat
      301 acccaccggc tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc
      361 tggggaaaag cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg
      421 aaggcgggtc aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac
      481 gacccaagcc tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga
      541 gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa
      601 ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg
      661 ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat
      721 agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct
      781 cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg
      841 ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca
      901 tggtgccgca accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag
      961 agaaatcggc attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc
     1021 gcatgaggcg tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca
     1081 cctcgtggcg cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa
     1141 gctcatcgga cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg
     1201 ctggatctcc gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga
     1261 ccgcgtggcg tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt
     1321 ggtggttgat cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat
     1381 cccgaccctg tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag
     1441 ccagcccggc attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg
     1501 ggaacggcgc gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg
     1561 cgagccgttg gagccgccga cacgggtcac gctgccgcgc cggtagcact tgggttgcgc
     1621 agcaacccgt aagtgcgctg ttccagacta tcggctgtag cccgcttgag accaccccag
     1681 gatacataga ttaccacaac tccgagccct tccacctagc gtgctaacca cgcacgctat
     1741 tgttgtatcc catataatgc aatcgtagtc tacctgactg tacttagaaa tgtggcttcg
     1801 cctttgccca cgcacctgat cgctcctcgt ttgcttttaa ggaccggacg aaccacagag
     1861 cattagaagg ctaggtggag gctcagtgat gataagtctg cgatggtgga tgcatgtgtc
     1921 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc agagggcaca atcctattcc
     1981 gcgctatccg acaatctcca agacattagg tggagttcag ttcggcgtat ggcatatgtc
     2041 gctggaaaga acatgtgagc aaaaggccag caaarnggcc tttttacggt tcctggcctn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaatag gattgtgccc tctgagcgga
     2221 taacaatttc acacaggaaa cagctatgac catgacacat gcatccacca tcgcagactt
     2281 atcatcactg agcctccacc tagcctatac aacaatagcg tgcgtggtta gcacgctagg
     2341 tggaagggct cggagttgtg gtaatctatg tatcctgggg tggtctcgag cgcccgtcac
     2401 tcccgtacta actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc
     2461 acgaacccct gcaataactg tcacgccccc aaacctgcaa acccagcagg ggcgggggct
     2521 ggcggggtgt tggaaaaatc catccatgat tatctaagaa taatccacta ggcgcggtta
     2581 tcagcgccct tgtggggcgc tgctgccctt gcccaatatg cccggccaga ggccggatag
     2641 ctggtctatt cgctgcgcta ggctacacac cgccccaccg ctgcgcggca gggggaaagg
     2701 cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga gcgcttttag
     2761 ccgctttagc ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg
     2821 cctgtctcgg tcgatcattc agcccggctc atccttctgg cgtggcggca gaccgaacaa
     2881 ggcgcggtcg tggtcgcgtt caaggtacgc atccattgcc gccatgagcc gatcctccgg
     2941 ccactcgctg ctgttcacct tggccaaaat catggccccc accagcacct tgcgccttgt
     3001 ttcgttcttg cgctcttgct gctgttccct tgcccgcacc cgctgaattt cggcattgat
     3061 tcgcgctcgt tgttcttcga gcttggccag ccgatccgcc gccttgttgc tccccttaac
     3121 catcttgaca ccccattgtt aatgtgctgt ctcgtaggct atcatggagg cacagcggcg
     3181 gcaatcccga ccctactttg taggggaggg cgcacttacc ggtttctctt cgagaaactg
     3241 gcctaacggc cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag
     3301 caaaagcaac agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg
     3361 tcgggcggcc aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc
     3421 cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg
     3481 cccgccgact actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag
     3541 gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc
     3601 gagttcgccc agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc
     3661 ggtggcggcg agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc
     3721 gagcggcccg ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg
     3781 gcacagaaga ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg
     3841 gccgaccatg ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga
     3901 acacttgagg cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg
     3961 gagatggaag gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc
     4021 gccaacgccc agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat
     4081 cgacagagtg aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc
     4141 ccagagcatg gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc
     4201 cagcgaggcg caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt
     4261 ggagctggcc aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc
     4321 ccggagcgcg tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat
     4381 gatgcctacg gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac
     4441 aaccgaggac ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg
     4501 caggccatag gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg
     4561 gacgccacca ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac
     4621 acgccatggc tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag
     4681 caggagcggc atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg
     4741 aaagccgagg gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca
     4801 tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg
     4861 gccagcgagt acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg
     4921 ggcttcacca accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg
     4981 ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc
     5041 cagcagatcg agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa
     5101 ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag
     5161 atggccgggc tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg
     5221 cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc
     5281 agcccagcgc tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc
     5341 agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg
     5401 gcaccccgcc agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgcttgcgt
     5461 tggtactcac gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac
     5521 gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc
     5581 ggtggctttg gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg
     5641 gccagcctta accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc
     5701 cccggcaagt ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct
     5761 gctggccagc cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg
     5821 gatgcgtcag cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct
     5881 ggaagaactc agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac
     5941 tgcctgcaaa ggaggcaatc aatggctacc cataagccta tcaatattct ggaggcgttc
     6001 gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca tggtggccgg tacggtcggg
     6061 gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg ccctgcaact ggccgcacag
     6121 attgcaggcg ggccggatct gctggaggtg ggcgaactgc ccaccggccc ggtgatctac
     6181 ctgcccgccg aagacccgcc caccgccatt catcaccgcc tgcacgccct tggggcgcac
     6241 ctcagcgccg aggaacggca agccgtggct gacggcctgc tgatccagcc gctgatcggc
     6301 agcctgccca acatcatggc cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc
     6361 cgccgcctga tggtgctgga cacgctgcgc cggttccaca tcgaggaaga aaacgccagc
     6421 ggccccatgg cccaggtcat cggtcgcatg gaggccatcg ccgccgatac cgggtgctct
     6481 atcgtgttcc tgcaccatgc cagcaagggc gcggccatga tgggcgcagg cgaccagcag
     6541 caggccagcc ggggcagctc ggtactggtc gataacatcc gctggcagtc ctacctgtcg
     6601 agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc
     6661 gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg
     6721 cggcatgacg gcggggtgct caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg
     6781 gtgccccgtg gtgaagccta agaacaagca cagcctcagc cacgtccggc acgacccggc
     6841 gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa
     6901 gctggacgtg acgtatgact acggcgacgg caagcggatc gagttcagcg gcccggagcc
     6961 gctgggcgct gatgatctgc gcatcctgca agggctggtg gccatggctg ggcctaatgg
     7021 cctagtgctt ggcccggaac ccaagaccga aggcggacgg cagctccggc tgttcctgga
     7081 acccaagtgg gaggccgtca ccgctgatgc catggtggtc aaaggtagct atcgggcgct
     7141 ggcaaaggaa atcggggcag aggtcgatag tggtggggcg ctcaagcaca tacaggactg
     7201 catcgagcgc ctttggaagg tatccatcat cgcccagaat ggccgcaagc ggcaggggtt
     7261 tcggctgctg tcggagtacg ccagcgacga ggcggacggg cgcctgtacg tggccctgaa
     7321 ccccttgatc gcgcaggccg tcatgggtgg cggccagcat gtgcgcatca gcatggacga
     7381 ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac cagcggctgt gtggctggat
     7441 cgaccccggc aaaaccggca aggcttccat agataccttg tgcggctatg tctggccgtc
     7501 agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg gtgcgcgagg cgttgccgga
     7561 gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg ggcaagtacg acatcacccg
     7621 gcccaaggcg gcaggctgac cccccccact ctattgtaaa caagacattt ttatctttta
     7681 tattcaatgg cttattttcc tgctaattgg taataccatg aaaaatacca tgctcagaaa
     7741 aggcttaaca atattttgaa aaattgccta ctgagcgctg ccgcacagct ccataggccg
     7801 ctttcctggc tttgcttcca gatgtatctc cggagacctc ggagacgaga cgagacagcc
     7861 tgagaatgga tgcgagtaat gatacaacaa tagcgtgctg ttcgcacgct aaggtcaggc
     7921 ggaatggcac ttccttattc cagatgcgtg cggattatgc atgactggcc gtcgttttac
     7981 aacgtcgtga ctgggaaaac cctggcgctt gtggtggatc acagttattg gagacttcct
     8041 tggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnctat tgttgtatca ttactcgcat ccattctcag gctgtctcgt
     8581 ctcgtctccg aggtctcagc ttgttaaggg atgcataaac tgcatccctt aacttgtttt
     8641 tcgtgtgcct attttttgtg aatcgmttat rkcytttssg cwgtmgsccg cttgagacca
     8701 ccccaggata catagattac cacaactccg agcccttcca cctagcgtgc taaccacgca
     8761 cgctattgtt gtatcccata taatgcaatc gtagtctacc tgactgtact tagaaatgtg
     8821 gcttcgcctt tgcccacgca cctgatcgct cctcgtttgc ttttaaggac cggacgaacc
     8881 acagagcatt agaaggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca
     8941 tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc
     9001 tattccgcgc tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca
     9061 tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9541 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctctattcaa caaagccgcc
     9781 gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat cgtcccccca tacgatataa
     9841 gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     9901 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     9961 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
    10021 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
    10081 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
    10141 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
    10201 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
    10261 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
    10321 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
    10381 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
    10441 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
    10501 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
    10561 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
    10621 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
    10681 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
    10741 ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
    10801 ccaccacaag cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca
    10861 taatccgcac gcatctggaa taaggaagtg ccattccgcc tgacct
//
