LOCUS       sequence-392747-        8824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0926
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1762..2350
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1815..1835)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2233..2244)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2422..2655
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2733..2854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2770..2789)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2824..2843
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2894..3029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3049..3068)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3050..3068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3078..3095)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3078..3094)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3087..3109)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3236..3691
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3323..3342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3533..3554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3717..3821
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3822..3890,3891..4682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4040..4059)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4853..5441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5342..5361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5595..5612
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5729..5750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5765..5770,5771..5788,5789..5795)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5803..5819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5808..5830
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5827..5843
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5827..5843
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5862..5882
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5864..5882
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5910..6136
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6137..6317
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6364..6489
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6982..7215
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7400..7444
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7675..7885
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7697..7893
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7744..7879
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7925..8527
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(7925..7944)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8421..8441
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8570..8687
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 gtcctgcaag cccagcggcc cgccggcggg agcgcgcgtt gcacccccgt gcgcgggcgg
     1261 caccgagtgc gcgggcacgt gcgccggggc cgggcggctg gagagcgcgg cgcgcaggcg
     1321 cctggcggcc aacgcgcgcg agcgccgccg catgcagggg ctcaacactg ccttcgaccg
     1381 cttacgcagg gtggttcccc agtggggcca ggataaaaag ctgtccaagt acgagaccct
     1441 gcagatggcc ctgagctaca tcatggctct gacccggatc ctggccgagg ccgagcgatt
     1501 cggctcggag cgggactggg tgggtctcca ctgtgagcac ttcggccgcg accactacct
     1561 cccgttcccg ggcgcgaagc tgccgggcga gagcgagctg tacagccaga gactcttcgg
     1621 cttccagccc gagcccttcc agatggccac ctagtaatga actagtccgt aacttgaaag
     1681 tatttcgatt tcttggcttt atatatcttg tggaaaggac gactatgtgt cgttgatttc
     1741 aacattacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     1801 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1861 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     1921 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     1981 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2041 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2101 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2161 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2221 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2281 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2341 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     2401 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2461 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2521 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2581 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2641 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     2701 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     2761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2821 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     2881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3001 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3061 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3601 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3661 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     3721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3781 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3841 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3901 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3961 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4021 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4081 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4141 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4201 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4261 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4321 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4381 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4441 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4501 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4561 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4621 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4681 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4741 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4801 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4861 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4921 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4981 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5041 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5101 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5161 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5221 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5281 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5341 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5401 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5461 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5521 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5581 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5641 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5701 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5761 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5821 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     5881 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     5941 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6001 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6061 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6121 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6181 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6241 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6301 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6361 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     6421 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     6481 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     6541 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     6601 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     6661 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     6721 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     6781 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     6841 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6901 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6961 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7021 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7081 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7141 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7201 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7261 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7321 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7381 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7441 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7501 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7561 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     7621 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7681 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7741 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7801 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7861 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     7921 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     7981 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8041 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8101 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     8161 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8221 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8281 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8341 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     8401 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     8461 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     8521 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     8581 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8641 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     8701 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     8761 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     8821 aatt
//
