LOCUS       sequence-392748-        8986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2499
FEATURES             Location/Qualifiers
     source          1..8986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1924..2512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1977..1997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2395..2406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2584..2817
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2895..3016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2932..2951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2986..3005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3056..3191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3211..3230)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3212..3230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3240..3257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3240..3256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3249..3271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3398..3853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3485..3504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3695..3716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3879..3983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3984..4052,4053..4844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4202..4221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5015..5603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5504..5523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5757..5774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5891..5912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5927..5932,5933..5950,5951..5957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5965..5981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5970..5992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5989..6005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5989..6005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6024..6044
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6026..6044
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6072..6298
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6299..6479
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6526..6651
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7144..7377
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7562..7606
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7837..8047
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7859..8055
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7906..8041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8087..8689
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8087..8106)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8583..8603
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8732..8849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 caataacttc tgccgggctc tggtggaccg gaggcctctg gggcccccca gctgcatgca
     1261 gctgggcgtc atgccccctc cccggcaggc gcccctgccc cccgccgagc ccctgggcaa
     1321 cgtgcccttc ctgctgtacc cgggcccagc agagccgccc tactacgacg cctatgcggg
     1381 ggtgttcccc tacgtgccct tccccggcgc cttcggggtc tacgaatacc ccttcgagcc
     1441 agccttcatc cagaagcgca acgagcgcga gaggcagcga gtcaagtgcg tcaacgaggg
     1501 ctacgctcgc ctccgcggcc acctccccgg cgccctggct gagaagcgac tcagcaaggt
     1561 ggagacgctg cgcgccgcca tccgctacat aaagtacctg caagagctgc tgagctcggc
     1621 ccccgacggc tcgacgcccc ccgcttcccg cggcctcccg ggcaccgggc cctgccccgc
     1681 gccgcccgcc accccccgtc ccgaccgccc tggcgacggc gaggcccggg cgccctcctc
     1741 cctggtgccg gagtcatccg agtcctcctg cttctccccg tcgcctttct tggagtcgga
     1801 ggaatcctgg cattagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     1861 ttatatatct tgtggaaagg acgacattag atgttgactg atccgcgcac gcgttaagtc
     1921 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     1981 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2041 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2101 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2161 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2221 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2281 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2341 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     2401 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     2461 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     2521 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2581 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     2641 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2701 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2761 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     2821 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     2881 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     2941 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3001 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3061 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3121 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3181 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3361 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     3421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3841 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5641 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5701 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5761 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5821 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5881 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5941 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6001 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6061 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     6121 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     6181 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     6241 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     6301 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6361 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6421 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6481 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     6541 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     6601 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     6661 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     6721 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     6781 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     6841 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     6901 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     6961 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     7021 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7081 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     7141 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     7201 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     7261 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7321 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     7381 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     7441 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     7501 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     7561 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     7621 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     7681 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     7741 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     7801 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7861 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7921 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7981 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8041 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     8101 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     8161 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     8221 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8281 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8341 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     8401 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     8461 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     8521 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     8581 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     8641 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
     8701 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     8761 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     8821 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     8881 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
     8941 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
