LOCUS       sequence-392749-        9673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2688
FEATURES             Location/Qualifiers
     source          1..9673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2611..3199
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2664..2684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3082..3093)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3271..3504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3582..3703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3619..3638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3673..3692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3743..3878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3898..3917)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3899..3917)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3927..3944)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3927..3943)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3936..3958)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4085..4540
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4172..4191)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4382..4403
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4566..4670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4671..4739,4740..5531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4889..4908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5702..6290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6191..6210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6444..6461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6578..6599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6614..6619,6620..6637,6638..6644)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6657..6679
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6676..6692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6676..6692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6711..6731
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6713..6731
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6759..6985
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6986..7166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7213..7338
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7831..8064
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8249..8293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8524..8734
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8546..8742
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8593..8728
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8774..9376
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8774..8793)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9270..9290
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9419..9536
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatagc
     1201 gtccaacagc tggaacgcca gcagcagccc cggggaggcc cgggaggatg ggcccgaggg
     1261 cctggacaag gggctggaca acgatgcgga gggcgtgtgg agcccggaca tcgagcagag
     1321 cttccaggag gccctggcca tctacccgcc ctgcggccgg cggaagatca tcctgtcaga
     1381 cgagggcaag atgtacggcc gaaatgagtt gattgcacgc tatattaaac tgaggacggg
     1441 gaagactcgg acgagaaaac aggtgtccag ccacatacag gttctagctc ggaagaaggt
     1501 gcgggagtac caggttggca tcaaggccat gaacctggac caggtctcca aggacaaagc
     1561 ccttcagagc atggcgtcca tgtcctctgc ccagatcgtc tctgccagtg tcctgcagaa
     1621 caagttcagc ccaccttccc ctctgcccca ggccgtcttc tccacttcct cgcggttctg
     1681 gagcagcccc cctctcctgg gacagcagcc tggaccctct caggacatca agccctttgc
     1741 acagccagcc taccccatcc agccgcccct gccgccgacg ctcagcagtt atgagcccct
     1801 ggccccgctc ccctcagctg ctgcctctgt gcctgtgtgg caggaccgta ccattgcctc
     1861 ctcccggctg cggctcctgg agtattcagc cttcatggag gtgcagcgag accctgacac
     1921 gtacagcaaa cacctgtttg tgcacatcgg ccagacgaac cccgccttct cagacccacc
     1981 cctggaggca gtagatgtgc gccagatcta tgacaaattc cccgagaaaa agggaggatt
     2041 gaaggagctc tatgagaagg ggccccctaa tgccttcttc cttgtcaagt tctgggccga
     2101 cctcaacagc accatccagg agggcccggg agccttctat ggggtcagct ctcagtacag
     2161 ctctgctgat agcatgacca tcagcgtctc caccaaggtg tgctcctttg gcaaacaggt
     2221 ggtagagaag gtggagactg agtatgccag gctggagaac gggcgctttg tgtaccgtat
     2281 ccaccgctcg cccatgtgcg agtacatgat caacttcatc cacaagctga agcacctgcc
     2341 cgagaagtac atgatgaaca gcgtgctgga gaacttcacc atcctgcagg tggtcacgag
     2401 ccgggactcc caggagaccc tgcttgtcat tgcttttgtc ttcgaagtct ccaccagtga
     2461 gcacggggcc cagcaccatg tctacaagct cgtcaaagac tagtaatgaa ctagtccgta
     2521 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg accctaagtc
     2581 cgggtcagat gtttgacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2641 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2701 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2761 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2821 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2881 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2941 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3001 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3061 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3121 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3181 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3241 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3301 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3361 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3421 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3481 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3541 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3721 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3841 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3901 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3961 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4021 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4081 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4141 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4201 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4261 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4321 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4381 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4441 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4501 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6481 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6541 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6601 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6661 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6721 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6781 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     6841 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     6901 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     6961 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7021 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7081 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7141 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7201 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7261 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7321 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7381 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7441 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7501 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7561 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7621 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7681 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7741 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7801 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     7861 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     7921 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7981 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8041 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8101 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8161 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8221 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8281 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8341 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8401 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8461 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8521 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8641 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8701 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     8761 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     8821 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     8881 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     8941 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9001 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9061 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9121 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9181 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9241 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9301 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9361 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9421 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9481 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9541 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9601 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9661 aggagtggga att
//
